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Conference Paper: Diversity of oral treponemes in patients with chronic periodontitis

TitleDiversity of oral treponemes in patients with chronic periodontitis
Authors
Issue Date2009
PublisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/
Citation
The 2nd Meeting of IADR Pan Asian Pacific Federation (PAPF) and the 1st Meeting of IADR Asia/Pacific Region (APR), Wuhan, China, 22-24 September 2009. In Journal of Dental Research, 2009, v. 88 n. Spec Iss B, p. 377 (PAPF/APR) How to Cite?
AbstractNumerous studies have demonstrated a strong association between periodontal disease and the presence of the oral spirochete Treponema denticola. Molecular analyses based on the sequence of 16S ribosomal RNA (rRNA) genes have revealed that over 60 different species or species-level phylotypes belonging to the genus Treponema are present within the oral cavity. However, the full extent of the diversity of oral Treponemes, as well as their possible pathogenic roles, remains to be established. Objectives: To gauge the diversity of Treponeme species within infected periodontal pockets of patients with chronic periodontitis. Methods: Subgingival plaque samples were collected from four separate diseased sites from three patients with chronic periodontitis. Total DNA was purified from the pooled samples from each patient, and near-full length regions of 16S rRNA genes were amplified by polymerase chain reaction (PCR) using previously-designed ‘treponeme specific' primers. PCR products were cloned into plasmids to form a library of treponeme-enriched 16S rRNA genes for each patient. 117 plasmids were sequenced in one or both directions to unambiguously identify the major species and phylotypes present in the three patients. Results: Fewer than 50% of plasmids sequenced contained Treponemal 16S rRNA genes. T. denticola. T. vincentii and T. socranskii were the dominant species detected, with several subspecies and novel phylotypes identified. T. medium, T. amylovorum, T. maltophilum and 3 novel species-level phylotypes of Treponema were also identified. Other species enriched using these ‘Treponeme-specific' primers belonged to the Olsenella, Atopobium, Cryptobacterium Flexistipes and Deferribacteraceae genera/families. Conclusions: Preliminary sequencing results from our Treponeme-enriched library of 16S rRNA genes from periodontitis patients have revealed significant diversity. We compare and contrast our results with those of related studies, and discuss the relative advantages and drawbacks associated with our ‘intensive and expensive' approach to characterizing oral treponeme populations.
Persistent Identifierhttp://hdl.handle.net/10722/94712
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorYou, M-
dc.contributor.authorMo, S-
dc.contributor.authorLeung, WK-
dc.contributor.authorWatt, RM-
dc.date.accessioned2010-09-25T15:39:38Z-
dc.date.available2010-09-25T15:39:38Z-
dc.date.issued2009-
dc.identifier.citationThe 2nd Meeting of IADR Pan Asian Pacific Federation (PAPF) and the 1st Meeting of IADR Asia/Pacific Region (APR), Wuhan, China, 22-24 September 2009. In Journal of Dental Research, 2009, v. 88 n. Spec Iss B, p. 377 (PAPF/APR)-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/94712-
dc.description.abstractNumerous studies have demonstrated a strong association between periodontal disease and the presence of the oral spirochete Treponema denticola. Molecular analyses based on the sequence of 16S ribosomal RNA (rRNA) genes have revealed that over 60 different species or species-level phylotypes belonging to the genus Treponema are present within the oral cavity. However, the full extent of the diversity of oral Treponemes, as well as their possible pathogenic roles, remains to be established. Objectives: To gauge the diversity of Treponeme species within infected periodontal pockets of patients with chronic periodontitis. Methods: Subgingival plaque samples were collected from four separate diseased sites from three patients with chronic periodontitis. Total DNA was purified from the pooled samples from each patient, and near-full length regions of 16S rRNA genes were amplified by polymerase chain reaction (PCR) using previously-designed ‘treponeme specific' primers. PCR products were cloned into plasmids to form a library of treponeme-enriched 16S rRNA genes for each patient. 117 plasmids were sequenced in one or both directions to unambiguously identify the major species and phylotypes present in the three patients. Results: Fewer than 50% of plasmids sequenced contained Treponemal 16S rRNA genes. T. denticola. T. vincentii and T. socranskii were the dominant species detected, with several subspecies and novel phylotypes identified. T. medium, T. amylovorum, T. maltophilum and 3 novel species-level phylotypes of Treponema were also identified. Other species enriched using these ‘Treponeme-specific' primers belonged to the Olsenella, Atopobium, Cryptobacterium Flexistipes and Deferribacteraceae genera/families. Conclusions: Preliminary sequencing results from our Treponeme-enriched library of 16S rRNA genes from periodontitis patients have revealed significant diversity. We compare and contrast our results with those of related studies, and discuss the relative advantages and drawbacks associated with our ‘intensive and expensive' approach to characterizing oral treponeme populations.-
dc.languageeng-
dc.publisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/-
dc.relation.ispartofJournal of Dental Research-
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.titleDiversity of oral treponemes in patients with chronic periodontitis-
dc.typeConference_Paper-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-0345&volume=88 &issue=Spec Iss B&spage=377 (PAPF/APR)&epage=&date=2009&atitle=Diversity+of+oral+treponemes+in+patients+with+chronic+periodontitisen_HK
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hk-
dc.identifier.emailWatt, RM: rmwatt@hkucc.hku.hk-
dc.identifier.authorityLeung, WK=rp00019-
dc.identifier.authorityWatt, RM=rp00043-
dc.identifier.hkuros169124-
dc.identifier.volume88-
dc.identifier.issueSpec Iss B-
dc.identifier.spage377 (PAPF/APR)-
dc.identifier.epage377 (PAPF/APR)-
dc.publisher.placeUnited States-

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