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Conference Paper: Infection efficiency of rat chondrocytes using different hybrids of recombinant adeno-associated virus vectors

TitleInfection efficiency of rat chondrocytes using different hybrids of recombinant adeno-associated virus vectors
Authors
Issue Date2006
PublisherOxford University Press
Citation
The 82nd Congress of the European Orthodontic Society, Vienna, Austria, 4 – 8 July 2006. In The European Journal of Orthodontics, 2006, v. 28 n. 6, p. e252-e253 Abstract no.277 How to Cite?
AbstractAIM: To investigate the best serotype of recombinant adeno-associated virus (rAAV) that leads to optimum chondrocyte infection, in an endeavour to create an appropriate delivery system for future gene therapy to regulate condylar growth. MATERIALS AND METHOD: rAAV2/1, rAAV2/2, rAAV2/5, rAAV2/6, and rAAV2/8 vectors expressing eGFP under the control of CAG (cytomegalovirus enhancer plus chicken β-actin) promoter were used to infect primary chondrocytes (isolated from rat mandibular condyles) at a multiplicity of infection of 5 × 104. The infection effi ciency for these cells with different hybrids was identifi ed using fl uorescence microscopy and fl ow cytometric analysis. RESULTS: All of the selected serotypes of RAAV infected rat chondrocytes and gave rise to GFP expression. The infection effi ciency was RAAV2/2 > RAAV2/5 > RAAV2/1 > RAAV2/8 = RAAV2/6. CONCLUSIONS: rAAV based vectors are capable of infecting rat chondrocytes. rAAV2/2 is the best serotype to infect chondrocytes in vitro and might thus be of interest for direct gene delivery to condylar cartilage in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/94694
ISSN
2015 Impact Factor: 1.44
2015 SCImago Journal Rankings: 1.090

 

DC FieldValueLanguage
dc.contributor.authorRabie, ABMen_HK
dc.contributor.authorDai, Jen_HK
dc.date.accessioned2010-09-25T15:39:05Z-
dc.date.available2010-09-25T15:39:05Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 82nd Congress of the European Orthodontic Society, Vienna, Austria, 4 – 8 July 2006. In The European Journal of Orthodontics, 2006, v. 28 n. 6, p. e252-e253 Abstract no.277-
dc.identifier.issn0141-5387-
dc.identifier.urihttp://hdl.handle.net/10722/94694-
dc.description.abstractAIM: To investigate the best serotype of recombinant adeno-associated virus (rAAV) that leads to optimum chondrocyte infection, in an endeavour to create an appropriate delivery system for future gene therapy to regulate condylar growth. MATERIALS AND METHOD: rAAV2/1, rAAV2/2, rAAV2/5, rAAV2/6, and rAAV2/8 vectors expressing eGFP under the control of CAG (cytomegalovirus enhancer plus chicken β-actin) promoter were used to infect primary chondrocytes (isolated from rat mandibular condyles) at a multiplicity of infection of 5 × 104. The infection effi ciency for these cells with different hybrids was identifi ed using fl uorescence microscopy and fl ow cytometric analysis. RESULTS: All of the selected serotypes of RAAV infected rat chondrocytes and gave rise to GFP expression. The infection effi ciency was RAAV2/2 > RAAV2/5 > RAAV2/1 > RAAV2/8 = RAAV2/6. CONCLUSIONS: rAAV based vectors are capable of infecting rat chondrocytes. rAAV2/2 is the best serotype to infect chondrocytes in vitro and might thus be of interest for direct gene delivery to condylar cartilage in vivo.-
dc.languageengen_HK
dc.publisherOxford University Press-
dc.relation.ispartofThe European Journal of Orthodonticsen_HK
dc.titleInfection efficiency of rat chondrocytes using different hybrids of recombinant adeno-associated virus vectorsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailRabie, ABM: rabie@hkusua.hku.hken_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/ejo/cjl095-
dc.identifier.hkuros125810en_HK

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