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Conference Paper: Effect of distraction rates on expression of tissue inhibitors of matrix metalloproteinases in rabbit mandibular distraction osteogenesis

TitleEffect of distraction rates on expression of tissue inhibitors of matrix metalloproteinases in rabbit mandibular distraction osteogenesis
Authors
Issue Date2005
PublisherElsevier
Citation
The 17th International Conference on Oral & Maxillofacial Surgery, Vienna, Austria, 29 August - 2 September 2005. In International Journal of Oral & Maxillofacial Surgery, 2005, v. 34 n. S1 p. 81 Abstract no.O44.1 How to Cite?
AbstractBone induction and ossification in distraction osteogenesis is related to the biological environment created by the mechanical tension. When compared with distraction at a normal rate, rapid distraction has demonstrated unreliable bone healing. Tissue inhibitors of matrix metalloproteinases (TIMPs) are nature inhibitors of matrix metalloproteinases (MMPs), which are capable of degrading bone matrix during the remodeling process. We hypothesize that different distraction rates may produce different TIMPs expression patterns, which will ultimately lead to different results in bone formation and remodeling. This study aims to compare the expression of endogenous TIMP-1 and -2 during rabbit mandibular distraction osteogenesis at normal and rapid rates. 27 New Zealand white rabbits were used. 24 rabbits were divided into 2 experimental groups with 12 rabbits in each group. Mandibular distraction was activated at 0.9mm/day lasting 12 days in the normal distraction group, and at 2.7 mm/day lasting 4 days in the rapid distraction group. 3 rabbits in each experimental group were sacrificed at day 1, week 1, week 2, and week 4 of consolidation. The other 3 rabbits without performing operation were used as control. Mandibular specimens were subjected for histological and Immunohistochemical assessment. The cells expressing TIMP-1 and -2 within the distraction regenerate were counted using a computer assisted image analyzing system. Histological study identified that normal rate distraction (0.9 mm/day) led to complete bone healing, whereas unreliable bone ossification was confirmed at rapid distraction (2.7 mm/day). The expression of TIMP-2 and -2 was localized in the osteoblasts, osteocytes and bone matrices of the trabeculae. Continuous up-regulation of TIMP-1 and -2 was observed in normal rate distraction group, whereas in the rapid lengthening model, TIMP-1 and -2 expression returned to a near-normal level at 4 weeks of consolidation. The change in the mechanical environment resulting from different rates of distraction leads to different expression of the TIMP-1 and -2 during mandibular distraction osteogenesis. The biological environment created by distraction at a normal rate is superior to the rapid distraction rate.
Persistent Identifierhttp://hdl.handle.net/10722/94600
ISSN
2015 Impact Factor: 1.563
2015 SCImago Journal Rankings: 0.854

 

DC FieldValueLanguage
dc.contributor.authorZheng, Len_HK
dc.contributor.authorMa, Len_HK
dc.contributor.authorCheung, LKen_HK
dc.date.accessioned2010-09-25T15:36:14Z-
dc.date.available2010-09-25T15:36:14Z-
dc.date.issued2005en_HK
dc.identifier.citationThe 17th International Conference on Oral & Maxillofacial Surgery, Vienna, Austria, 29 August - 2 September 2005. In International Journal of Oral & Maxillofacial Surgery, 2005, v. 34 n. S1 p. 81 Abstract no.O44.1-
dc.identifier.issn0901-5027-
dc.identifier.urihttp://hdl.handle.net/10722/94600-
dc.description.abstractBone induction and ossification in distraction osteogenesis is related to the biological environment created by the mechanical tension. When compared with distraction at a normal rate, rapid distraction has demonstrated unreliable bone healing. Tissue inhibitors of matrix metalloproteinases (TIMPs) are nature inhibitors of matrix metalloproteinases (MMPs), which are capable of degrading bone matrix during the remodeling process. We hypothesize that different distraction rates may produce different TIMPs expression patterns, which will ultimately lead to different results in bone formation and remodeling. This study aims to compare the expression of endogenous TIMP-1 and -2 during rabbit mandibular distraction osteogenesis at normal and rapid rates. 27 New Zealand white rabbits were used. 24 rabbits were divided into 2 experimental groups with 12 rabbits in each group. Mandibular distraction was activated at 0.9mm/day lasting 12 days in the normal distraction group, and at 2.7 mm/day lasting 4 days in the rapid distraction group. 3 rabbits in each experimental group were sacrificed at day 1, week 1, week 2, and week 4 of consolidation. The other 3 rabbits without performing operation were used as control. Mandibular specimens were subjected for histological and Immunohistochemical assessment. The cells expressing TIMP-1 and -2 within the distraction regenerate were counted using a computer assisted image analyzing system. Histological study identified that normal rate distraction (0.9 mm/day) led to complete bone healing, whereas unreliable bone ossification was confirmed at rapid distraction (2.7 mm/day). The expression of TIMP-2 and -2 was localized in the osteoblasts, osteocytes and bone matrices of the trabeculae. Continuous up-regulation of TIMP-1 and -2 was observed in normal rate distraction group, whereas in the rapid lengthening model, TIMP-1 and -2 expression returned to a near-normal level at 4 weeks of consolidation. The change in the mechanical environment resulting from different rates of distraction leads to different expression of the TIMP-1 and -2 during mandibular distraction osteogenesis. The biological environment created by distraction at a normal rate is superior to the rapid distraction rate.-
dc.languageengen_HK
dc.publisherElsevier-
dc.relation.ispartofInternational Journal of Oral & Maxillofacial Surgeryen_HK
dc.titleEffect of distraction rates on expression of tissue inhibitors of matrix metalloproteinases in rabbit mandibular distraction osteogenesisen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailZheng, L: zhengl@graduate.hku.hken_HK
dc.identifier.emailCheung, LK: lkcheung@hkucc.hku.hken_HK
dc.identifier.authorityCheung, LK=rp00013en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/s0901-5027(05)81196-8-
dc.identifier.hkuros124075en_HK

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