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Conference Paper: Bacterial endotoxin LPS but not LTA can enhance osteogenic differentiation of human mesenchymal stem cells

TitleBacterial endotoxin LPS but not LTA can enhance osteogenic differentiation of human mesenchymal stem cells
Authors
Issue Date2005
PublisherAmerican Society of Hematology.
Citation
Blood, 2005, v. 106 n. 11, p. 4230 How to Cite?
AbstractBackground & Objective: Dental implant requires osseointegration for anchoring and human’s oral cavity has plenty of bacterial oral flora. Whether these bacteria have any effects on the human mesenchymal Stem Cells (MSCs) that can differentiate into osteoblasts remains unknown. We therefore investigated the effect of bacterial endotoxins commonly found in the oral cavity and gastrointestinal tract, namely lipopolysaccharides (LPS, Escherichia coli) and lipoteichoic acid (LTA, Streptococcus pyogenes), on the proliferation and osteogenic differentiation of MSCs. Methods: Human MSCs are derived from bone marrow (BM) of normal healthy donors. The culture condition, immunophenotyping determination and tests of differentiating functions of the human MSCs were similar to what we reported previously (Li J, Br J Haematol 2004). The proliferation of MSCs under either a 3-day or a prolonged 7-day endotoxins challenge was evaluated by XTT assay. The extent of osteogenic differentiation was examined under microscopy and measured by the increase in alkaline phosphatase (ALP) activity at day 10 and the calcium mineralization/deposition per unit volume of protein at day 14. Results: There was no significant effect of LPS and LTA on the growth and proliferation of MSCs, even under a relatively high dose. However, continued LPS challenge on MSCs under osteogenic culture condition was shown to increase the ALP activity and calcium deposition in a dose-dependent manner (100ng/ml, 1 ug/ml, 10ug/ml). No such phenomenon can be identified when LTA challenge was used. Conclusions: LPS and LTA did not show any significant effect on the proliferation and growth of human MSCs. However, LPS enhanced the osteogenic differentiation of MSCs in a dose-dependent manner. Our finding suggests that the endotoxin from bacteria commonly found in the oral cavity and gut does not have any negative impact on MSCs induced osteogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/94343
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395

 

DC FieldValueLanguage
dc.contributor.authorChan, GCFen_HK
dc.contributor.authorMo, FYen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorLaw, HKWen_HK
dc.contributor.authorLau, YLen_HK
dc.date.accessioned2010-09-25T15:28:35Z-
dc.date.available2010-09-25T15:28:35Z-
dc.date.issued2005en_HK
dc.identifier.citationBlood, 2005, v. 106 n. 11, p. 4230en_HK
dc.identifier.issn0006-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/94343-
dc.description.abstractBackground & Objective: Dental implant requires osseointegration for anchoring and human’s oral cavity has plenty of bacterial oral flora. Whether these bacteria have any effects on the human mesenchymal Stem Cells (MSCs) that can differentiate into osteoblasts remains unknown. We therefore investigated the effect of bacterial endotoxins commonly found in the oral cavity and gastrointestinal tract, namely lipopolysaccharides (LPS, Escherichia coli) and lipoteichoic acid (LTA, Streptococcus pyogenes), on the proliferation and osteogenic differentiation of MSCs. Methods: Human MSCs are derived from bone marrow (BM) of normal healthy donors. The culture condition, immunophenotyping determination and tests of differentiating functions of the human MSCs were similar to what we reported previously (Li J, Br J Haematol 2004). The proliferation of MSCs under either a 3-day or a prolonged 7-day endotoxins challenge was evaluated by XTT assay. The extent of osteogenic differentiation was examined under microscopy and measured by the increase in alkaline phosphatase (ALP) activity at day 10 and the calcium mineralization/deposition per unit volume of protein at day 14. Results: There was no significant effect of LPS and LTA on the growth and proliferation of MSCs, even under a relatively high dose. However, continued LPS challenge on MSCs under osteogenic culture condition was shown to increase the ALP activity and calcium deposition in a dose-dependent manner (100ng/ml, 1 ug/ml, 10ug/ml). No such phenomenon can be identified when LTA challenge was used. Conclusions: LPS and LTA did not show any significant effect on the proliferation and growth of human MSCs. However, LPS enhanced the osteogenic differentiation of MSCs in a dose-dependent manner. Our finding suggests that the endotoxin from bacteria commonly found in the oral cavity and gut does not have any negative impact on MSCs induced osteogenesis.-
dc.languageengen_HK
dc.publisherAmerican Society of Hematology.en_HK
dc.relation.ispartofBlooden_HK
dc.titleBacterial endotoxin LPS but not LTA can enhance osteogenic differentiation of human mesenchymal stem cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=106&issue=11&spage=138b&epage=&date=2005&atitle=Bacterial+Endotoxin+LPS+but+Not+LTA+Can+Enhance+Osteogenic+Differentiation+of+Human+Mesenchymal+Stell+Cellsen_HK
dc.identifier.emailChan, GCF: gcfchan@hkucc.hku.hken_HK
dc.identifier.emailMo, FY: h0106002@graduate.hku.hken_HK
dc.identifier.emailYip, HK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.emailLaw, HKW: hkwlaw@hkucc.hku.hken_HK
dc.identifier.emailLau, YL: lauylung@hkucc.hku.hken_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.identifier.authorityYip, HK=rp00027en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.identifier.hkuros113021en_HK
dc.identifier.hkuros145787-
dc.identifier.volume106en_HK
dc.identifier.issue11en_HK
dc.identifier.spage138en_HK

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