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Conference Paper: Gene therapy to enhance condylar growth

TitleGene therapy to enhance condylar growth
Authors
Issue Date2007
PublisherOxford University Press
Citation
The 83rd Congress of the European Orthodontic Society, Berlin, Germany, 20-24 July 2007. In The European Journal of Orthodontics, 2007, v. 29 n. 5, e31-e32 Abstract no.70 How to Cite?
AbstractAIM: Craniofacial anomalies resulting from impaired growth of the mandibular condyles require multidisciplinary intervention, which imposes a substantial burden on patients and their families. Correcting such deformities with alternative strategy-gene therapy is still uncharted territory. The aim of this research was to enhance mandibular condylar growth using gene therapy specifi cally to establish an effective in vivo gene delivery system with recombinant adeno-associated virus (rAAV)-mediated vascular endothelial growth factor (VEGF). MATERIALS AND METHOD: In situ hybridization, RT-PCR, immunostaining and western blot, transgene expression were used to detect the presence of VEGF in the mandibular condyles of 90 Sprague Dawely rats throughout the experimental period. RESULTS: At defi ned time points specifi c osteogenetic markers (ALP and osteocalcin) and chongenetic markers (collagen type II and collagen type X) were assessed by means of biochemical techniques and their expression signifi cantly changed from day 30. Proliferation index by PCNA staining also showed a signifi cant increase in cell proliferation. Morphological measurement identifi ed that the size of mandibular condyle signifi cantly increased from day 30. CONCLUSION: rAAV-VEGF is an effi cient delivery system to induce mandibular condylar growth. This work provides the basis for future gene therapy to treat patients with craniofacial deformities.
Persistent Identifierhttp://hdl.handle.net/10722/94258
ISSN
2015 Impact Factor: 1.44
2015 SCImago Journal Rankings: 1.090

 

DC FieldValueLanguage
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2010-09-25T15:26:04Z-
dc.date.available2010-09-25T15:26:04Z-
dc.date.issued2007en_HK
dc.identifier.citationThe 83rd Congress of the European Orthodontic Society, Berlin, Germany, 20-24 July 2007. In The European Journal of Orthodontics, 2007, v. 29 n. 5, e31-e32 Abstract no.70-
dc.identifier.issn0141-5387-
dc.identifier.urihttp://hdl.handle.net/10722/94258-
dc.description.abstractAIM: Craniofacial anomalies resulting from impaired growth of the mandibular condyles require multidisciplinary intervention, which imposes a substantial burden on patients and their families. Correcting such deformities with alternative strategy-gene therapy is still uncharted territory. The aim of this research was to enhance mandibular condylar growth using gene therapy specifi cally to establish an effective in vivo gene delivery system with recombinant adeno-associated virus (rAAV)-mediated vascular endothelial growth factor (VEGF). MATERIALS AND METHOD: In situ hybridization, RT-PCR, immunostaining and western blot, transgene expression were used to detect the presence of VEGF in the mandibular condyles of 90 Sprague Dawely rats throughout the experimental period. RESULTS: At defi ned time points specifi c osteogenetic markers (ALP and osteocalcin) and chongenetic markers (collagen type II and collagen type X) were assessed by means of biochemical techniques and their expression signifi cantly changed from day 30. Proliferation index by PCNA staining also showed a signifi cant increase in cell proliferation. Morphological measurement identifi ed that the size of mandibular condyle signifi cantly increased from day 30. CONCLUSION: rAAV-VEGF is an effi cient delivery system to induce mandibular condylar growth. This work provides the basis for future gene therapy to treat patients with craniofacial deformities.-
dc.languageengen_HK
dc.publisherOxford University Press-
dc.relation.ispartofThe European Journal of Orthodonticsen_HK
dc.titleGene therapy to enhance condylar growthen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailRabie, ABM: rabie@hkusua.hku.hken_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/ejo/cjm091-
dc.identifier.hkuros135865en_HK

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