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Conference Paper: Microarray analysis of chondrogenic genes in condylar cartilage

TitleMicroarray analysis of chondrogenic genes in condylar cartilage
Authors
Issue Date2006
PublisherOxford University Press
Citation
The 82nd Congress of the European Orthodontic Society, Vienna, Austria, 4-8 July 2006. In The European Journal of Orthodontics, 2006, v. 28 n. 6, p. e209 Abstract no.161 How to Cite?
AbstractAIM: To investigate chondrogenic gene expression changes in mandibular condylar cartilage under mechanical strain. MATERIALS AND METHOD: Two hundred and eighty, 35-day-old, female Sprague-Dawley rats randomly divided into seven experimental and seven control groups. The experimental groups were fi tted with bite-jumping appliances. Each group of rats was killed on the following experimental days: 1, 3, 7, 9, 14, 30 and 33. Immediately after sacrifi ce, the condyles were dissected and total RNA was extracted to oligonucleotide microarray gene chips containing 15,923 genes. After a series of microarray data analyses, genes were excluded if absent across all time points in both groups. All genes that were either unchanged or whose expression was changed less than ×2 in relation to the control group were excluded from further analyses. This selection resulted in a net of 1,082 genes, 666 were increased in expression, and 416 decreased in expression, at least a two-fold changed. This group of genes was further analyzed using hierarchical clustering and selforganizing maps, and resulted in the identifi cation of numerous genes not previously known to be regulated in condylar cartilage during chondrogenesis under mechanical strain. RESULTS: Sixteen genes were involved in chondrogenesis. To focus on these genes that were more specifi c to chondrogenesis, the results were validated by both RT-PCR and immunostaining. CONCLUSIONS: Using microarray technology to analyze the gene expression of mandibular condylar cartilage under mechanical strain, some chondrogenic regulating factors involved in condylar cartilage growth were identified.
Persistent Identifierhttp://hdl.handle.net/10722/94093
ISSN
2015 Impact Factor: 1.44
2015 SCImago Journal Rankings: 1.090

 

DC FieldValueLanguage
dc.contributor.authorHagg, EUOen_HK
dc.contributor.authorSong, Yen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2010-09-25T15:21:10Z-
dc.date.available2010-09-25T15:21:10Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 82nd Congress of the European Orthodontic Society, Vienna, Austria, 4-8 July 2006. In The European Journal of Orthodontics, 2006, v. 28 n. 6, p. e209 Abstract no.161-
dc.identifier.issn0141-5387-
dc.identifier.urihttp://hdl.handle.net/10722/94093-
dc.description.abstractAIM: To investigate chondrogenic gene expression changes in mandibular condylar cartilage under mechanical strain. MATERIALS AND METHOD: Two hundred and eighty, 35-day-old, female Sprague-Dawley rats randomly divided into seven experimental and seven control groups. The experimental groups were fi tted with bite-jumping appliances. Each group of rats was killed on the following experimental days: 1, 3, 7, 9, 14, 30 and 33. Immediately after sacrifi ce, the condyles were dissected and total RNA was extracted to oligonucleotide microarray gene chips containing 15,923 genes. After a series of microarray data analyses, genes were excluded if absent across all time points in both groups. All genes that were either unchanged or whose expression was changed less than ×2 in relation to the control group were excluded from further analyses. This selection resulted in a net of 1,082 genes, 666 were increased in expression, and 416 decreased in expression, at least a two-fold changed. This group of genes was further analyzed using hierarchical clustering and selforganizing maps, and resulted in the identifi cation of numerous genes not previously known to be regulated in condylar cartilage during chondrogenesis under mechanical strain. RESULTS: Sixteen genes were involved in chondrogenesis. To focus on these genes that were more specifi c to chondrogenesis, the results were validated by both RT-PCR and immunostaining. CONCLUSIONS: Using microarray technology to analyze the gene expression of mandibular condylar cartilage under mechanical strain, some chondrogenic regulating factors involved in condylar cartilage growth were identified.-
dc.languageengen_HK
dc.publisherOxford University Press-
dc.relation.ispartofThe European Journal of Orthodonticsen_HK
dc.titleMicroarray analysis of chondrogenic genes in condylar cartilageen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailHagg, EUO: euohagg@hkusua.hku.hken_HK
dc.identifier.emailRabie, ABM: rabie@hkusua.hku.hken_HK
dc.identifier.authorityHagg, EUO=rp00020en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/ejo/cjl095-
dc.identifier.hkuros125811en_HK
dc.identifier.hkuros117142-

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