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Article: A non-toxic herbal remedy which enhance lymphocyte activity and cytokine secretion: Ganoderma lucidum

TitleA non-toxic herbal remedy which enhance lymphocyte activity and cytokine secretion: Ganoderma lucidum
Authors
KeywordsCell metabolism
Cell proliferation
Cytokines
Ganoderma lucidum
Immune system
Toxicity
Issue Date2008
PublisherAcademic Journals. The Journal's web site is located at http://www.academicjournals.org/AJB/index.htm
Citation
African Journal Of Biotechnology, 2008, v. 7 n. 22, p. 4010-4014 How to Cite?
AbstractGanoderma lucidum is grown in the deep foliage of South-western China, where it is free from heavy metals and pollutions from populated cities. G. lucidum contains biologically active components, polysaccharides, which have been shown to enhance the immune system. In China, G. lucidum is commonly used to treat cancer, allergies, inflammation, hypertension and platelet-aggregation. This study investigates the proliferation, metabolism and cytokine secretion of lymphocytes in vitro upon G. lucidum stimulation. The powder of G. lucidum was provided by Mei Shan Tang Limited (Hong Kong) and tested to be free of heavy metals and microorganisms. Lymphocytes were extracted from 66 healthy human subjects and cultured with a pre-determined optimal concentration of G. lucidum (50-100 ug/mL) or a control medium for 72 h. Proliferation was measured with cell proliferation reagent, and metabolic rate was measured with ATP bioluminescence assay. Levels of IL-2, IL-4, IL-8, IL-10, GM-CSF and TNF-α were measured with ELISA. Meanwhile, toxicity was tested by feeding 70 rats with G. lucidum. No significant toxicity was detected in the rats. In human lymphocytes, G. lucidum was found to significantly increase cell proliferation and metabolism (p<0.05). T lymphocytes also demonstrated aggregation after G. lucidum treatment, which indicated T lymphocyte transformation. G. lucidum-treated cells also demonstrated a significantly increased secretion of IL-2, IL-8 and TNF-α (p<0.05). G. lucidum has the ability to enhance lymphocyte proliferation and metabolism without any significant toxicity in vitro. It also increases the secretion of cytokines, thus suggesting the potential of enhancing cellular immunity. G. lucidum is therefore potential candidate as a safe immunostimulant for patients on antin-eoplastic therapy. © 2008 Academic Journals.
Persistent Identifierhttp://hdl.handle.net/10722/92814
ISSN
2010 Impact Factor: 0.573
2015 SCImago Journal Rankings: 0.295
References

 

DC FieldValueLanguage
dc.contributor.authorLiang, Hen_HK
dc.contributor.authorLoo, WTYen_HK
dc.contributor.authorYeung, BHSen_HK
dc.contributor.authorCheung, MNBen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorChen, JPen_HK
dc.date.accessioned2010-09-17T10:58:01Z-
dc.date.available2010-09-17T10:58:01Z-
dc.date.issued2008en_HK
dc.identifier.citationAfrican Journal Of Biotechnology, 2008, v. 7 n. 22, p. 4010-4014en_HK
dc.identifier.issn1684-5315en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92814-
dc.description.abstractGanoderma lucidum is grown in the deep foliage of South-western China, where it is free from heavy metals and pollutions from populated cities. G. lucidum contains biologically active components, polysaccharides, which have been shown to enhance the immune system. In China, G. lucidum is commonly used to treat cancer, allergies, inflammation, hypertension and platelet-aggregation. This study investigates the proliferation, metabolism and cytokine secretion of lymphocytes in vitro upon G. lucidum stimulation. The powder of G. lucidum was provided by Mei Shan Tang Limited (Hong Kong) and tested to be free of heavy metals and microorganisms. Lymphocytes were extracted from 66 healthy human subjects and cultured with a pre-determined optimal concentration of G. lucidum (50-100 ug/mL) or a control medium for 72 h. Proliferation was measured with cell proliferation reagent, and metabolic rate was measured with ATP bioluminescence assay. Levels of IL-2, IL-4, IL-8, IL-10, GM-CSF and TNF-α were measured with ELISA. Meanwhile, toxicity was tested by feeding 70 rats with G. lucidum. No significant toxicity was detected in the rats. In human lymphocytes, G. lucidum was found to significantly increase cell proliferation and metabolism (p<0.05). T lymphocytes also demonstrated aggregation after G. lucidum treatment, which indicated T lymphocyte transformation. G. lucidum-treated cells also demonstrated a significantly increased secretion of IL-2, IL-8 and TNF-α (p<0.05). G. lucidum has the ability to enhance lymphocyte proliferation and metabolism without any significant toxicity in vitro. It also increases the secretion of cytokines, thus suggesting the potential of enhancing cellular immunity. G. lucidum is therefore potential candidate as a safe immunostimulant for patients on antin-eoplastic therapy. © 2008 Academic Journals.en_HK
dc.languageengen_HK
dc.publisherAcademic Journals. The Journal's web site is located at http://www.academicjournals.org/AJB/index.htmen_HK
dc.relation.ispartofAfrican Journal of Biotechnologyen_HK
dc.subjectCell metabolismen_HK
dc.subjectCell proliferationen_HK
dc.subjectCytokinesen_HK
dc.subjectGanoderma lucidumen_HK
dc.subjectImmune systemen_HK
dc.subjectToxicityen_HK
dc.titleA non-toxic herbal remedy which enhance lymphocyte activity and cytokine secretion: Ganoderma lucidumen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, JP: abchen@hku.hken_HK
dc.identifier.authorityChen, JP=rp01316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-57149090745en_HK
dc.identifier.hkuros155516-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57149090745&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue22en_HK
dc.identifier.spage4010en_HK
dc.identifier.epage4014en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiang, H=51161531700en_HK
dc.identifier.scopusauthoridLoo, WTY=7003567474en_HK
dc.identifier.scopusauthoridYeung, BHS=25958468800en_HK
dc.identifier.scopusauthoridCheung, MNB=7201897548en_HK
dc.identifier.scopusauthoridWang, M=36080215600en_HK
dc.identifier.scopusauthoridChen, JP=22733695400en_HK

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