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Article: Effects of Shugansanjie Tang on matrix metalloproteinases 1, 3 and 9 and telomerase reverse transcriptase expression in human breast cells in vitro

TitleEffects of Shugansanjie Tang on matrix metalloproteinases 1, 3 and 9 and telomerase reverse transcriptase expression in human breast cells in vitro
Authors
KeywordsCell proliferation
Immunostimulatory toxicity
Shugansajei Tang
Telomerase reverse transcriptase
Issue Date2007
PublisherElsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/biopha
Citation
Biomedicine And Pharmacotherapy, 2007, v. 61 n. 9 SPEC. ISS., p. 601-605 How to Cite?
AbstractBackground: The traditional Chinese medication Shugansanjie Tang (SGT), whose active component is Akebia Trifoliate Koidz, possesses potential anti-tumor and immunostimulatory effects especially for breast cancer. The invasive processes of such cancers have been attributed to matrix metalloproteinases (MMPs) which may be the key factor mediating this process. Telomerase reverse transcriptase (TERT) catalyze the lengthening of telomeres, which prolongs cell life and interrupts natural cell death. The aim of this study is to determine the effects of Shugansanjie Tang on MMP levels and TERT activity using breast cancer cell lines. Materials and methods: We used the breast cancer cell lines MDA-MB-231 and BT-483. Cell inhibition rate was measured by WST-1 reagent, cell apoptosis by Apoptotic DNA Ladder Kit, mRNA expression of MMP-1, MMP-3 and MMP-9 by PCR and TERT by immunohistochemistry stain. Results: Comparing to the control group, the test group showed lower cell growth rate, decreased mRNA expression of MMP-1, MMP-3 and MMP-9 production and less intense staining of MMPs with diaminobenzidine tetrahydrochloride. Conclusion: This study demonstrated that Shugansanjie Tang inhibits the growth of breast cancer cells by apoptosis and lowers the level of certain matrix metalloproteinases and activity of telomerase reverse transcriptase in breast cancer in vitro. © 2007 Elsevier Masson SAS. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92811
ISSN
2015 Impact Factor: 2.326
2015 SCImago Journal Rankings: 0.787
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLoo, WTYen_HK
dc.contributor.authorChen, JPen_HK
dc.contributor.authorChow, LWCen_HK
dc.contributor.authorChou, JWKen_HK
dc.date.accessioned2010-09-17T10:57:55Z-
dc.date.available2010-09-17T10:57:55Z-
dc.date.issued2007en_HK
dc.identifier.citationBiomedicine And Pharmacotherapy, 2007, v. 61 n. 9 SPEC. ISS., p. 601-605en_HK
dc.identifier.issn0753-3322en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92811-
dc.description.abstractBackground: The traditional Chinese medication Shugansanjie Tang (SGT), whose active component is Akebia Trifoliate Koidz, possesses potential anti-tumor and immunostimulatory effects especially for breast cancer. The invasive processes of such cancers have been attributed to matrix metalloproteinases (MMPs) which may be the key factor mediating this process. Telomerase reverse transcriptase (TERT) catalyze the lengthening of telomeres, which prolongs cell life and interrupts natural cell death. The aim of this study is to determine the effects of Shugansanjie Tang on MMP levels and TERT activity using breast cancer cell lines. Materials and methods: We used the breast cancer cell lines MDA-MB-231 and BT-483. Cell inhibition rate was measured by WST-1 reagent, cell apoptosis by Apoptotic DNA Ladder Kit, mRNA expression of MMP-1, MMP-3 and MMP-9 by PCR and TERT by immunohistochemistry stain. Results: Comparing to the control group, the test group showed lower cell growth rate, decreased mRNA expression of MMP-1, MMP-3 and MMP-9 production and less intense staining of MMPs with diaminobenzidine tetrahydrochloride. Conclusion: This study demonstrated that Shugansanjie Tang inhibits the growth of breast cancer cells by apoptosis and lowers the level of certain matrix metalloproteinases and activity of telomerase reverse transcriptase in breast cancer in vitro. © 2007 Elsevier Masson SAS. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/biophaen_HK
dc.relation.ispartofBiomedicine and Pharmacotherapyen_HK
dc.subjectCell proliferationen_HK
dc.subjectImmunostimulatory toxicityen_HK
dc.subjectShugansajei Tangen_HK
dc.subjectTelomerase reverse transcriptaseen_HK
dc.titleEffects of Shugansanjie Tang on matrix metalloproteinases 1, 3 and 9 and telomerase reverse transcriptase expression in human breast cells in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, JP: abchen@hku.hken_HK
dc.identifier.authorityChen, JP=rp01316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biopha.2007.08.024en_HK
dc.identifier.pmid17913446-
dc.identifier.scopuseid_2-s2.0-35248866354en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35248866354&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume61en_HK
dc.identifier.issue9 SPEC. ISS.en_HK
dc.identifier.spage601en_HK
dc.identifier.epage605en_HK
dc.identifier.isiWOS:000251067600018-
dc.publisher.placeFranceen_HK
dc.identifier.scopusauthoridLoo, WTY=7003567474en_HK
dc.identifier.scopusauthoridChen, JP=22733695400en_HK
dc.identifier.scopusauthoridChow, LWC=34975216600en_HK
dc.identifier.scopusauthoridChou, JWK=35081716000en_HK

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