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Article: Application of solid phase microextraction in the determination of paralytic shellfish poisoning toxins

TitleApplication of solid phase microextraction in the determination of paralytic shellfish poisoning toxins
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2005
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/analyst
Citation
Analyst, 2005, v. 130 n. 11, p. 1524-1529 How to Cite?
AbstractA SPME-HPLC-post-column fluorescent derivatization method for the direct determination of saxitoxin (STX), the most potent paralytic shellfish poisoning (PSP) toxin, in water has been developed. Commercially available SPME devices with 50 μm Carbowax templated resin (CW/TPR) coating was found to be able to pre-concentrate STX from aqueous media. A special pre-conditioning treatment of soaking the SPME coating in 0.1 M NaOH solution significantly improved the extraction efficiency. The optimal pH for the SPME process is 8.1 and the equilibration time is 40 min. The partition coefficient, K, of the distribution of STX between the SPME coating and the aqueous media was measured to be 2.99 ± 0.04 × 103. Extracted toxin on the SPME stationary phase was difficult to be desorbed by the HPLC mobile phase under dynamic desorption mode. A static ion-pairing desorption technique using a desorption solvent mixture of 20 mM sodium 1-heptanesulfonate in 30% aqueous acetonitrile acidified with 50 mM sulfuric acid was developed to overcome this problem. The method detection limit and repeatability achieved by this SPME-HPLC method were 0.11 ng ml-1 and 3.7%, respectively, with a sample volume of just 5 ml of water. This analytical method is adequate for the monitoring of the PSP toxin in fresh/drinking waters. However, serious interference was observed when this technique was applied to saline water samples. This is probably due to competition of sodium ions with the cationic STX for absorption into the SPME stationary phase. © The Royal Society of Chemistry 2005.
Persistent Identifierhttp://hdl.handle.net/10722/92807
ISSN
2015 Impact Factor: 4.033
2015 SCImago Journal Rankings: 1.300
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, IOMen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorCheung, RHYen_HK
dc.contributor.authorLam, MHWen_HK
dc.contributor.authorWu, RSSen_HK
dc.date.accessioned2010-09-17T10:57:44Z-
dc.date.available2010-09-17T10:57:44Z-
dc.date.issued2005en_HK
dc.identifier.citationAnalyst, 2005, v. 130 n. 11, p. 1524-1529en_HK
dc.identifier.issn0003-2654en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92807-
dc.description.abstractA SPME-HPLC-post-column fluorescent derivatization method for the direct determination of saxitoxin (STX), the most potent paralytic shellfish poisoning (PSP) toxin, in water has been developed. Commercially available SPME devices with 50 μm Carbowax templated resin (CW/TPR) coating was found to be able to pre-concentrate STX from aqueous media. A special pre-conditioning treatment of soaking the SPME coating in 0.1 M NaOH solution significantly improved the extraction efficiency. The optimal pH for the SPME process is 8.1 and the equilibration time is 40 min. The partition coefficient, K, of the distribution of STX between the SPME coating and the aqueous media was measured to be 2.99 ± 0.04 × 103. Extracted toxin on the SPME stationary phase was difficult to be desorbed by the HPLC mobile phase under dynamic desorption mode. A static ion-pairing desorption technique using a desorption solvent mixture of 20 mM sodium 1-heptanesulfonate in 30% aqueous acetonitrile acidified with 50 mM sulfuric acid was developed to overcome this problem. The method detection limit and repeatability achieved by this SPME-HPLC method were 0.11 ng ml-1 and 3.7%, respectively, with a sample volume of just 5 ml of water. This analytical method is adequate for the monitoring of the PSP toxin in fresh/drinking waters. However, serious interference was observed when this technique was applied to saline water samples. This is probably due to competition of sodium ions with the cationic STX for absorption into the SPME stationary phase. © The Royal Society of Chemistry 2005.en_HK
dc.languageengen_HK
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/analysten_HK
dc.relation.ispartofAnalysten_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleApplication of solid phase microextraction in the determination of paralytic shellfish poisoning toxinsen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/b506324hen_HK
dc.identifier.pmid16222375-
dc.identifier.scopuseid_2-s2.0-27744452921en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27744452921&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume130en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1524en_HK
dc.identifier.epage1529en_HK
dc.identifier.isiWOS:000232502100012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChan, IOM=9239010800en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridCheung, RHY=9239011000en_HK
dc.identifier.scopusauthoridLam, MHW=7202630175en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK

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