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Article: Cellular and molecular basis of cadmium-induced deformities in zebrafish embryos

TitleCellular and molecular basis of cadmium-induced deformities in zebrafish embryos
Authors
KeywordsCadmium
Developmental defects
Gene expression
Myosin
Sonic hedgehog
Issue Date2000
PublisherSociety of Environmental Toxicology and Chemistry. The Journal's web site is located at http://etc.allenpress.com/
Citation
Environmental Toxicology And Chemistry, 2000, v. 19 n. 12, p. 3024-3031 How to Cite?
AbstractCadmium is known to cause developmental defects in a variety of vertebrate species, but relatively little is known about the underlying molecular mechanisms. In this study, we used zebrafish (Danio rerio) embryos as a model system to investigate cadmium-induced toxicities. Fertilized embryos collected at 5-h after fertilization were incubated for 18 h in culture media containing 1 to 1,000 μM CdCl 2. The median embryolethal concentration (LC50) was 168 μM, whereas the median effect concentration (EC50) for total adverse effect (mortality and developmental defects) was 138 μM. Six major types of deformities were observed: head and eye hypoplasia, hypopigmentation, cardiac edema, yolk sac abnormalities, altered axial curvature, and tail malformations. The frequency of malformations increased with cadmium concentration. Somites of embryos with altered axial curvature were investigated using the antimyosin antibody MF-20. This study demonstrated, to our knowledge for the first time, reduced myotome formation in cadmium-induced spinal deformity. Embryos with head and eye hypoplasia were studied using the anti-neural tissue antibody zns-2, and a poorly developed central nervous system was revealed. Head and eye hypoplasia were associated with lack of expression of the sonic hedgehog gene, which controls the patterning of the neural tube and somites. Genes involved in tail formations, such as evenskipped 1 and no tail, were ectopically expressed in embryos with tail malformations. Our data support the hypothesis that fish embryonic malformations induced by cadmium might be mediated through ectopic expression of developmental regulatory genes.
Persistent Identifierhttp://hdl.handle.net/10722/92806
ISSN
2015 Impact Factor: 2.763
2015 SCImago Journal Rankings: 1.523
References

 

DC FieldValueLanguage
dc.contributor.authorShuk Han Chengen_HK
dc.contributor.authorWai, AWKen_HK
dc.contributor.authorChun Hung Soen_HK
dc.contributor.authorWu, RSSen_HK
dc.date.accessioned2010-09-17T10:57:42Z-
dc.date.available2010-09-17T10:57:42Z-
dc.date.issued2000en_HK
dc.identifier.citationEnvironmental Toxicology And Chemistry, 2000, v. 19 n. 12, p. 3024-3031en_HK
dc.identifier.issn0730-7268en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92806-
dc.description.abstractCadmium is known to cause developmental defects in a variety of vertebrate species, but relatively little is known about the underlying molecular mechanisms. In this study, we used zebrafish (Danio rerio) embryos as a model system to investigate cadmium-induced toxicities. Fertilized embryos collected at 5-h after fertilization were incubated for 18 h in culture media containing 1 to 1,000 μM CdCl 2. The median embryolethal concentration (LC50) was 168 μM, whereas the median effect concentration (EC50) for total adverse effect (mortality and developmental defects) was 138 μM. Six major types of deformities were observed: head and eye hypoplasia, hypopigmentation, cardiac edema, yolk sac abnormalities, altered axial curvature, and tail malformations. The frequency of malformations increased with cadmium concentration. Somites of embryos with altered axial curvature were investigated using the antimyosin antibody MF-20. This study demonstrated, to our knowledge for the first time, reduced myotome formation in cadmium-induced spinal deformity. Embryos with head and eye hypoplasia were studied using the anti-neural tissue antibody zns-2, and a poorly developed central nervous system was revealed. Head and eye hypoplasia were associated with lack of expression of the sonic hedgehog gene, which controls the patterning of the neural tube and somites. Genes involved in tail formations, such as evenskipped 1 and no tail, were ectopically expressed in embryos with tail malformations. Our data support the hypothesis that fish embryonic malformations induced by cadmium might be mediated through ectopic expression of developmental regulatory genes.en_HK
dc.languageengen_HK
dc.publisherSociety of Environmental Toxicology and Chemistry. The Journal's web site is located at http://etc.allenpress.com/en_HK
dc.relation.ispartofEnvironmental Toxicology and Chemistryen_HK
dc.subjectCadmiumen_HK
dc.subjectDevelopmental defectsen_HK
dc.subjectGene expressionen_HK
dc.subjectMyosinen_HK
dc.subjectSonic hedgehogen_HK
dc.titleCellular and molecular basis of cadmium-induced deformities in zebrafish embryosen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0034515285en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034515285&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue12en_HK
dc.identifier.spage3024en_HK
dc.identifier.epage3031en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShuk Han Cheng=23052581400en_HK
dc.identifier.scopusauthoridWai, AWK=36799005500en_HK
dc.identifier.scopusauthoridChun Hung So=7409951316en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK

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