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Article: Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)

TitleInduction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)
Authors
KeywordsApoptosis
Caspases
Oxidative stress
Perfluorinated organic compounds
Primary cultured hepatocytes
Reactive oxygen species
Tilapia
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2007, v. 82 n. 2, p. 135-143 How to Cite?
AbstractPerfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92803
ISSN
2015 Impact Factor: 3.557
2015 SCImago Journal Rankings: 1.671
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Cen_HK
dc.contributor.authorYu, Ken_HK
dc.contributor.authorShi, Xen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorZhou, Ben_HK
dc.date.accessioned2010-09-17T10:57:37Z-
dc.date.available2010-09-17T10:57:37Z-
dc.date.issued2007en_HK
dc.identifier.citationAquatic Toxicology, 2007, v. 82 n. 2, p. 135-143en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92803-
dc.description.abstractPerfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. © 2007 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectApoptosisen_HK
dc.subjectCaspasesen_HK
dc.subjectOxidative stressen_HK
dc.subjectPerfluorinated organic compoundsen_HK
dc.subjectPrimary cultured hepatocytesen_HK
dc.subjectReactive oxygen speciesen_HK
dc.subjectTilapiaen_HK
dc.titleInduction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)en_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2007.02.006en_HK
dc.identifier.pmid17374408-
dc.identifier.scopuseid_2-s2.0-33947586193en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33947586193&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume82en_HK
dc.identifier.issue2en_HK
dc.identifier.spage135en_HK
dc.identifier.epage143en_HK
dc.identifier.isiWOS:000245962400006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLiu, C=8318316300en_HK
dc.identifier.scopusauthoridYu, K=35757266000en_HK
dc.identifier.scopusauthoridShi, X=16067334700en_HK
dc.identifier.scopusauthoridWang, J=8941425500en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridZhou, B=7401906781en_HK

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