Article: Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China

File Download Links for fulltext
(May Require Subscription)
Supplementary
  • Basic View
  • Metadata View
  • XML View
TitleUse of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China
AuthorsXu, LH2
Lam, PKS3
Chen, JP2
Xu, JM2
Wong, BSF3
Zhang, YY2
Wu, RSS3
Harada, KI1
KeywordsSpecies Index: Algae
Anabaena
Cyanobacteria
Oscillatoria
Issue Date2000
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
CitationChemosphere, 2000, v. 41 n. 1-2, p. 53-58 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0045-6535(99)00389-6
AbstractSeasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.
ISSN0045-6535
2011 Impact Factor: 3.206
2011 SCImago Journal Rankings: 0.155
DOIhttp://dx.doi.org/10.1016/S0045-6535(99)00389-6
ISI Accession Number IDWOS:000086786500008
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorXu, LH
dc.contributor.authorLam, PKS
dc.contributor.authorChen, JP
dc.contributor.authorXu, JM
dc.contributor.authorWong, BSF
dc.contributor.authorZhang, YY
dc.contributor.authorWu, RSS
dc.contributor.authorHarada, KI
dc.date.accessioned2010-09-17T10:57:33Z
dc.date.available2010-09-17T10:57:33Z
dc.date.issued2000
dc.description.abstractSeasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationChemosphere, 2000, v. 41 n. 1-2, p. 53-58 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0045-6535(99)00389-6
dc.identifier.doihttp://dx.doi.org/10.1016/S0045-6535(99)00389-6
dc.identifier.epage58
dc.identifier.isiWOS:000086786500008
dc.identifier.issn0045-6535
2011 Impact Factor: 3.206
2011 SCImago Journal Rankings: 0.155
dc.identifier.issue1-2
dc.identifier.pmid10819179
dc.identifier.scopuseid_2-s2.0-0342859372
dc.identifier.spage53
dc.identifier.urihttp://hdl.handle.net/10722/92801
dc.identifier.volume41
dc.languageeng
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
dc.publisher.placeUnited Kingdom
dc.relation.ispartofChemosphere
dc.relation.referencesReferences in Scopus
dc.subjectSpecies Index: Algae
dc.subjectAnabaena
dc.subjectCyanobacteria
dc.subjectOscillatoria
dc.titleUse of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China
dc.typeArticle
Author Affiliations
  1. Meijo University
  2. Institute of Hydrobiology, Chinese Academy of Sciences
  3. Ctr. Environ. Sci. Technol.