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Article: Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China
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TitleUse of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China
 
AuthorsXu, LH2
Lam, PKS3
Chen, JP2
Xu, JM2
Wong, BSF3
Zhang, YY2
Wu, RSS3
Harada, KI1
 
KeywordsSpecies Index: Algae
Anabaena
Cyanobacteria
Oscillatoria
 
Issue Date2000
 
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
 
CitationChemosphere, 2000, v. 41 n. 1-2, p. 53-58 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0045-6535(99)00389-6
 
AbstractSeasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.
 
ISSN0045-6535
2013 Impact Factor: 3.499
2013 SCImago Journal Rankings: 1.746
 
DOIhttp://dx.doi.org/10.1016/S0045-6535(99)00389-6
 
ISI Accession Number IDWOS:000086786500008
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorXu, LH
 
dc.contributor.authorLam, PKS
 
dc.contributor.authorChen, JP
 
dc.contributor.authorXu, JM
 
dc.contributor.authorWong, BSF
 
dc.contributor.authorZhang, YY
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorHarada, KI
 
dc.date.accessioned2010-09-17T10:57:33Z
 
dc.date.available2010-09-17T10:57:33Z
 
dc.date.issued2000
 
dc.description.abstractSeasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationChemosphere, 2000, v. 41 n. 1-2, p. 53-58 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0045-6535(99)00389-6
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0045-6535(99)00389-6
 
dc.identifier.epage58
 
dc.identifier.isiWOS:000086786500008
 
dc.identifier.issn0045-6535
2013 Impact Factor: 3.499
2013 SCImago Journal Rankings: 1.746
 
dc.identifier.issue1-2
 
dc.identifier.pmid10819179
 
dc.identifier.scopuseid_2-s2.0-0342859372
 
dc.identifier.spage53
 
dc.identifier.urihttp://hdl.handle.net/10722/92801
 
dc.identifier.volume41
 
dc.languageeng
 
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofChemosphere
 
dc.relation.referencesReferences in Scopus
 
dc.subjectSpecies Index: Algae
 
dc.subjectAnabaena
 
dc.subjectCyanobacteria
 
dc.subjectOscillatoria
 
dc.titleUse of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China
 
dc.typeArticle
 
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<contributor.author>Wong, BSF</contributor.author>
<contributor.author>Zhang, YY</contributor.author>
<contributor.author>Wu, RSS</contributor.author>
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Author Affiliations
  1. Meijo University
  2. Institute of Hydrobiology, Chinese Academy of Sciences
  3. Ctr. Environ. Sci. Technol.