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Article: Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China

TitleUse of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China
Authors
KeywordsSpecies Index: Algae
Anabaena
Cyanobacteria
Oscillatoria
Issue Date2000
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
Citation
Chemosphere, 2000, v. 41 n. 1-2, p. 53-58 How to Cite?
Abstract
Seasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/92801
ISSN
2013 Impact Factor: 3.499
2013 SCImago Journal Rankings: 1.746
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, LHen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorChen, JPen_HK
dc.contributor.authorXu, JMen_HK
dc.contributor.authorWong, BSFen_HK
dc.contributor.authorZhang, YYen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorHarada, KIen_HK
dc.date.accessioned2010-09-17T10:57:33Z-
dc.date.available2010-09-17T10:57:33Z-
dc.date.issued2000en_HK
dc.identifier.citationChemosphere, 2000, v. 41 n. 1-2, p. 53-58en_HK
dc.identifier.issn0045-6535en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92801-
dc.description.abstractSeasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphereen_HK
dc.relation.ispartofChemosphereen_HK
dc.subjectSpecies Index: Algaeen_HK
dc.subjectAnabaenaen_HK
dc.subjectCyanobacteriaen_HK
dc.subjectOscillatoriaen_HK
dc.titleUse of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in Chinaen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0045-6535(99)00389-6en_HK
dc.identifier.pmid10819179en_HK
dc.identifier.scopuseid_2-s2.0-0342859372en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0342859372&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume41en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage53en_HK
dc.identifier.epage58en_HK
dc.identifier.isiWOS:000086786500008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridXu, LH=7404745586en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridChen, JP=7501883737en_HK
dc.identifier.scopusauthoridXu, JM=7407010329en_HK
dc.identifier.scopusauthoridWong, BSF=7402021987en_HK
dc.identifier.scopusauthoridZhang, YY=7601321199en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridHarada, KI=35403962700en_HK

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