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Article: Production of reactive oxygen species and 8-hydroxy-2′deoxyguanosine in KB cells co-exposed to benzo[a]pyrene and UV-A radiation

TitleProduction of reactive oxygen species and 8-hydroxy-2′deoxyguanosine in KB cells co-exposed to benzo[a]pyrene and UV-A radiation
Authors
Keywords8-Hydroxy-2 ′deoxyguanosine
Benzo[a]pyrene
DNA integrity
Oxidative stress
Reactive oxygen species
Ultraviolet A
Issue Date2004
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
Citation
Chemosphere, 2004, v. 55 n. 10, p. 1303-1308 How to Cite?
AbstractPrevious studies have shown that ultraviolet (UV) A light and the polycyclic aromatic hydrocarbon benzo[a]pyrene (BaP) can synergistically enhance the formation of 8-hydroxy-2′deoxyguanosine (8-OHdG) in living cells. It has been postulated that the underlying mechanism is production of reactive oxygen species (ROS) via photosensitization, but direct evidence supporting this hypothesis has been lacking. This study examined intracellular ROS production in living cells co-exposed to UV-A and BaP as well as the relationship between intracellular production of ROS and formation of 8-OHdG. KB cells were exposed to BaP for 24 h, followed by exposure to UV-A (365 nm) or UV-B (312 nm). The levels of intracellular ROS were directly measured by use of the fluorescent probe dihydrorhodamine 123 (DHR-123) in flow cytometry. Levels of 8-OHdG were measured by high performance liquid chromatography coupled with electrochemical detection (HPLC-ECD). The results demonstrated that UV-B itself induced a much greater level of intracellular ROS than did UV-A alone under the same dose of energy (0.10 mW/cm2, 20 min). The presence of BaP (13.3 μM) substantially increased ROS production in UV-A-treated cells (2.9-fold), but only slightly enhanced ROS production in UV-B-treated cells (1.3-fold). These results show that BaP acts mainly as a photosensitizer of UV-A, but not UV-B. Furthermore, greater intracellular ROS production was proportional to both BaP concentration and UV-A dosage. There was a linear relationship between ROS production and 8-OHdG formation in cells co-exposed to BaP and UV-A. Results of this study suggest that UV-A and BaP act synergistically to enhance ROS production and formation of 8-OHdG, resulting in increased DNA damage. © 2004 Published by Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/92791
ISSN
2015 Impact Factor: 3.698
2015 SCImago Journal Rankings: 1.536
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorFu, Wen_HK
dc.contributor.authorXu, Len_HK
dc.contributor.authorLam, PKSen_HK
dc.date.accessioned2010-09-17T10:57:16Z-
dc.date.available2010-09-17T10:57:16Z-
dc.date.issued2004en_HK
dc.identifier.citationChemosphere, 2004, v. 55 n. 10, p. 1303-1308en_HK
dc.identifier.issn0045-6535en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92791-
dc.description.abstractPrevious studies have shown that ultraviolet (UV) A light and the polycyclic aromatic hydrocarbon benzo[a]pyrene (BaP) can synergistically enhance the formation of 8-hydroxy-2′deoxyguanosine (8-OHdG) in living cells. It has been postulated that the underlying mechanism is production of reactive oxygen species (ROS) via photosensitization, but direct evidence supporting this hypothesis has been lacking. This study examined intracellular ROS production in living cells co-exposed to UV-A and BaP as well as the relationship between intracellular production of ROS and formation of 8-OHdG. KB cells were exposed to BaP for 24 h, followed by exposure to UV-A (365 nm) or UV-B (312 nm). The levels of intracellular ROS were directly measured by use of the fluorescent probe dihydrorhodamine 123 (DHR-123) in flow cytometry. Levels of 8-OHdG were measured by high performance liquid chromatography coupled with electrochemical detection (HPLC-ECD). The results demonstrated that UV-B itself induced a much greater level of intracellular ROS than did UV-A alone under the same dose of energy (0.10 mW/cm2, 20 min). The presence of BaP (13.3 μM) substantially increased ROS production in UV-A-treated cells (2.9-fold), but only slightly enhanced ROS production in UV-B-treated cells (1.3-fold). These results show that BaP acts mainly as a photosensitizer of UV-A, but not UV-B. Furthermore, greater intracellular ROS production was proportional to both BaP concentration and UV-A dosage. There was a linear relationship between ROS production and 8-OHdG formation in cells co-exposed to BaP and UV-A. Results of this study suggest that UV-A and BaP act synergistically to enhance ROS production and formation of 8-OHdG, resulting in increased DNA damage. © 2004 Published by Elsevier Ltd.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphereen_HK
dc.relation.ispartofChemosphereen_HK
dc.subject8-Hydroxy-2 ′deoxyguanosineen_HK
dc.subjectBenzo[a]pyreneen_HK
dc.subjectDNA integrityen_HK
dc.subjectOxidative stressen_HK
dc.subjectReactive oxygen speciesen_HK
dc.subjectUltraviolet Aen_HK
dc.titleProduction of reactive oxygen species and 8-hydroxy-2′deoxyguanosine in KB cells co-exposed to benzo[a]pyrene and UV-A radiationen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chemosphere.2003.12.004en_HK
dc.identifier.pmid15081772-
dc.identifier.scopuseid_2-s2.0-1842687869en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1842687869&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume55en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1303en_HK
dc.identifier.epage1308en_HK
dc.identifier.isiWOS:000221501700002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhang, X=8606600100en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridFu, W=7202947073en_HK
dc.identifier.scopusauthoridXu, L=7404745586en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK

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