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Article: The H295R system for evaluation of endocrine-disrupting effects
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TitleThe H295R system for evaluation of endocrine-disrupting effects
 
AuthorsGracia, T1
Hilscherova, K1
Jones, PD1
Newsted, JL4
Zhang, X1 5
Hecker, M1
Higley, EB1
Sanderson, JT3
Yu, RMK5
Wu, RSS5
Giesy, JP5 1 2
 
KeywordsBioassay
Endocrine disruptors
Mixtures
Screening
Steroidogenesis
 
Issue Date2006
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv
 
CitationEcotoxicology And Environmental Safety, 2006, v. 65 n. 3, p. 293-305 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ecoenv.2006.06.012
 
AbstractThe present studies were undertaken to evaluate the utility of the H295R system as an in vitro assay to assess the potential of chemicals to modulate steroidogenesis. The effects of four model chemicals on the expression of ten steroidogenic genes and on the production of three steroid hormones were examined. Exposures with individual model chemicals as well as binary mixtures were conducted. Although the responses reflect the known mode of action of the various compounds, the results show that designating a chemical as "specific inducer or inhibitor" is unwise. Not all changes in the mixture exposures could be predicted based on results from individual chemical exposures. Hormone production was not always directly related to gene expression. The H295R system integrates the effects of direct-acting hormone agonists and antagonists as well as chemicals affecting signal transduction pathways for steroid production and provides data on both gene expression and hormone secretion which makes this cell line a valuable tool to examine effects of chemicals on steroidogenesis. © 2006 Elsevier Inc. All rights reserved.
 
ISSN0147-6513
2013 Impact Factor: 2.482
 
DOIhttp://dx.doi.org/10.1016/j.ecoenv.2006.06.012
 
ISI Accession Number IDWOS:000242446500001
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorGracia, T
 
dc.contributor.authorHilscherova, K
 
dc.contributor.authorJones, PD
 
dc.contributor.authorNewsted, JL
 
dc.contributor.authorZhang, X
 
dc.contributor.authorHecker, M
 
dc.contributor.authorHigley, EB
 
dc.contributor.authorSanderson, JT
 
dc.contributor.authorYu, RMK
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorGiesy, JP
 
dc.date.accessioned2010-09-17T10:57:00Z
 
dc.date.available2010-09-17T10:57:00Z
 
dc.date.issued2006
 
dc.description.abstractThe present studies were undertaken to evaluate the utility of the H295R system as an in vitro assay to assess the potential of chemicals to modulate steroidogenesis. The effects of four model chemicals on the expression of ten steroidogenic genes and on the production of three steroid hormones were examined. Exposures with individual model chemicals as well as binary mixtures were conducted. Although the responses reflect the known mode of action of the various compounds, the results show that designating a chemical as "specific inducer or inhibitor" is unwise. Not all changes in the mixture exposures could be predicted based on results from individual chemical exposures. Hormone production was not always directly related to gene expression. The H295R system integrates the effects of direct-acting hormone agonists and antagonists as well as chemicals affecting signal transduction pathways for steroid production and provides data on both gene expression and hormone secretion which makes this cell line a valuable tool to examine effects of chemicals on steroidogenesis. © 2006 Elsevier Inc. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationEcotoxicology And Environmental Safety, 2006, v. 65 n. 3, p. 293-305 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ecoenv.2006.06.012
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ecoenv.2006.06.012
 
dc.identifier.eissn1090-2414
 
dc.identifier.epage305
 
dc.identifier.isiWOS:000242446500001
 
dc.identifier.issn0147-6513
2013 Impact Factor: 2.482
 
dc.identifier.issue3
 
dc.identifier.pmid16935330
 
dc.identifier.scopuseid_2-s2.0-33749338632
 
dc.identifier.spage293
 
dc.identifier.urihttp://hdl.handle.net/10722/92782
 
dc.identifier.volume65
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv
 
dc.publisher.placeUnited States
 
dc.relation.ispartofEcotoxicology and Environmental Safety
 
dc.relation.referencesReferences in Scopus
 
dc.subjectBioassay
 
dc.subjectEndocrine disruptors
 
dc.subjectMixtures
 
dc.subjectScreening
 
dc.subjectSteroidogenesis
 
dc.titleThe H295R system for evaluation of endocrine-disrupting effects
 
dc.typeArticle
 
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<contributor.author>Newsted, JL</contributor.author>
<contributor.author>Zhang, X</contributor.author>
<contributor.author>Hecker, M</contributor.author>
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Author Affiliations
  1. Center for Integrative Toxicology
  2. University of Saskatchewan
  3. Le réseau de l'Université du Québec
  4. ENTRIX Inc.
  5. City University of Hong Kong