Article: The H295R system for evaluation of endocrine-disrupting effects

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TitleThe H295R system for evaluation of endocrine-disrupting effects
AuthorsGracia, T1
Hilscherova, K1
Jones, PD1
Newsted, JL4
Zhang, X1 5
Hecker, M1
Higley, EB1
Sanderson, JT3
Yu, RMK5
Wu, RSS5
Giesy, JP1 2 5
KeywordsBioassay
Endocrine disruptors
Mixtures
Screening
Steroidogenesis
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv
CitationEcotoxicology And Environmental Safety, 2006, v. 65 n. 3, p. 293-305 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ecoenv.2006.06.012
AbstractThe present studies were undertaken to evaluate the utility of the H295R system as an in vitro assay to assess the potential of chemicals to modulate steroidogenesis. The effects of four model chemicals on the expression of ten steroidogenic genes and on the production of three steroid hormones were examined. Exposures with individual model chemicals as well as binary mixtures were conducted. Although the responses reflect the known mode of action of the various compounds, the results show that designating a chemical as "specific inducer or inhibitor" is unwise. Not all changes in the mixture exposures could be predicted based on results from individual chemical exposures. Hormone production was not always directly related to gene expression. The H295R system integrates the effects of direct-acting hormone agonists and antagonists as well as chemicals affecting signal transduction pathways for steroid production and provides data on both gene expression and hormone secretion which makes this cell line a valuable tool to examine effects of chemicals on steroidogenesis. © 2006 Elsevier Inc. All rights reserved.
ISSN0147-6513
2011 Impact Factor: 2.294
2011 SCImago Journal Rankings: 0.096
DOIhttp://dx.doi.org/10.1016/j.ecoenv.2006.06.012
ISI Accession Number IDWOS:000242446500001
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorGracia, T
dc.contributor.authorHilscherova, K
dc.contributor.authorJones, PD
dc.contributor.authorNewsted, JL
dc.contributor.authorZhang, X
dc.contributor.authorHecker, M
dc.contributor.authorHigley, EB
dc.contributor.authorSanderson, JT
dc.contributor.authorYu, RMK
dc.contributor.authorWu, RSS
dc.contributor.authorGiesy, JP
dc.date.accessioned2010-09-17T10:57:00Z
dc.date.available2010-09-17T10:57:00Z
dc.date.issued2006
dc.description.abstractThe present studies were undertaken to evaluate the utility of the H295R system as an in vitro assay to assess the potential of chemicals to modulate steroidogenesis. The effects of four model chemicals on the expression of ten steroidogenic genes and on the production of three steroid hormones were examined. Exposures with individual model chemicals as well as binary mixtures were conducted. Although the responses reflect the known mode of action of the various compounds, the results show that designating a chemical as "specific inducer or inhibitor" is unwise. Not all changes in the mixture exposures could be predicted based on results from individual chemical exposures. Hormone production was not always directly related to gene expression. The H295R system integrates the effects of direct-acting hormone agonists and antagonists as well as chemicals affecting signal transduction pathways for steroid production and provides data on both gene expression and hormone secretion which makes this cell line a valuable tool to examine effects of chemicals on steroidogenesis. © 2006 Elsevier Inc. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationEcotoxicology And Environmental Safety, 2006, v. 65 n. 3, p. 293-305 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ecoenv.2006.06.012
dc.identifier.doihttp://dx.doi.org/10.1016/j.ecoenv.2006.06.012
dc.identifier.eissn1090-2414
dc.identifier.epage305
dc.identifier.isiWOS:000242446500001
dc.identifier.issn0147-6513
2011 Impact Factor: 2.294
2011 SCImago Journal Rankings: 0.096
dc.identifier.issue3
dc.identifier.pmid16935330
dc.identifier.scopuseid_2-s2.0-33749338632
dc.identifier.spage293
dc.identifier.urihttp://hdl.handle.net/10722/92782
dc.identifier.volume65
dc.languageeng
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv
dc.publisher.placeUnited States
dc.relation.ispartofEcotoxicology and Environmental Safety
dc.relation.referencesReferences in Scopus
dc.subjectBioassay
dc.subjectEndocrine disruptors
dc.subjectMixtures
dc.subjectScreening
dc.subjectSteroidogenesis
dc.titleThe H295R system for evaluation of endocrine-disrupting effects
dc.typeArticle
Author Affiliations
  1. Center for Integrative Toxicology
  2. University of Saskatchewan
  3. Le réseau de l'Université du Québec
  4. ENTRIX Inc.
  5. City University of Hong Kong