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Article: The H295R system for evaluation of endocrine-disrupting effects

TitleThe H295R system for evaluation of endocrine-disrupting effects
Authors
KeywordsBioassay
Endocrine disruptors
Mixtures
Screening
Steroidogenesis
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv
Citation
Ecotoxicology And Environmental Safety, 2006, v. 65 n. 3, p. 293-305 How to Cite?
Abstract
The present studies were undertaken to evaluate the utility of the H295R system as an in vitro assay to assess the potential of chemicals to modulate steroidogenesis. The effects of four model chemicals on the expression of ten steroidogenic genes and on the production of three steroid hormones were examined. Exposures with individual model chemicals as well as binary mixtures were conducted. Although the responses reflect the known mode of action of the various compounds, the results show that designating a chemical as "specific inducer or inhibitor" is unwise. Not all changes in the mixture exposures could be predicted based on results from individual chemical exposures. Hormone production was not always directly related to gene expression. The H295R system integrates the effects of direct-acting hormone agonists and antagonists as well as chemicals affecting signal transduction pathways for steroid production and provides data on both gene expression and hormone secretion which makes this cell line a valuable tool to examine effects of chemicals on steroidogenesis. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92782
ISSN
2013 Impact Factor: 2.482
ISI Accession Number ID
References

 

Author Affiliations
  1. Center for Integrative Toxicology
  2. University of Saskatchewan
  3. Le réseau de l'Université du Québec
  4. ENTRIX Inc.
  5. City University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorGracia, Ten_HK
dc.contributor.authorHilscherova, Ken_HK
dc.contributor.authorJones, PDen_HK
dc.contributor.authorNewsted, JLen_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorHecker, Men_HK
dc.contributor.authorHigley, EBen_HK
dc.contributor.authorSanderson, JTen_HK
dc.contributor.authorYu, RMKen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorGiesy, JPen_HK
dc.date.accessioned2010-09-17T10:57:00Z-
dc.date.available2010-09-17T10:57:00Z-
dc.date.issued2006en_HK
dc.identifier.citationEcotoxicology And Environmental Safety, 2006, v. 65 n. 3, p. 293-305en_HK
dc.identifier.issn0147-6513en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92782-
dc.description.abstractThe present studies were undertaken to evaluate the utility of the H295R system as an in vitro assay to assess the potential of chemicals to modulate steroidogenesis. The effects of four model chemicals on the expression of ten steroidogenic genes and on the production of three steroid hormones were examined. Exposures with individual model chemicals as well as binary mixtures were conducted. Although the responses reflect the known mode of action of the various compounds, the results show that designating a chemical as "specific inducer or inhibitor" is unwise. Not all changes in the mixture exposures could be predicted based on results from individual chemical exposures. Hormone production was not always directly related to gene expression. The H295R system integrates the effects of direct-acting hormone agonists and antagonists as well as chemicals affecting signal transduction pathways for steroid production and provides data on both gene expression and hormone secretion which makes this cell line a valuable tool to examine effects of chemicals on steroidogenesis. © 2006 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenven_HK
dc.relation.ispartofEcotoxicology and Environmental Safetyen_HK
dc.subjectBioassayen_HK
dc.subjectEndocrine disruptorsen_HK
dc.subjectMixturesen_HK
dc.subjectScreeningen_HK
dc.subjectSteroidogenesisen_HK
dc.titleThe H295R system for evaluation of endocrine-disrupting effectsen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ecoenv.2006.06.012en_HK
dc.identifier.pmid16935330en_HK
dc.identifier.scopuseid_2-s2.0-33749338632en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33749338632&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume65en_HK
dc.identifier.issue3en_HK
dc.identifier.spage293en_HK
dc.identifier.epage305en_HK
dc.identifier.eissn1090-2414-
dc.identifier.isiWOS:000242446500001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGracia, T=6506058618en_HK
dc.identifier.scopusauthoridHilscherova, K=6603438103en_HK
dc.identifier.scopusauthoridJones, PD=34771015600en_HK
dc.identifier.scopusauthoridNewsted, JL=6603677236en_HK
dc.identifier.scopusauthoridZhang, X=36086912900en_HK
dc.identifier.scopusauthoridHecker, M=35247848500en_HK
dc.identifier.scopusauthoridHigley, EB=13004073100en_HK
dc.identifier.scopusauthoridSanderson, JT=7004489858en_HK
dc.identifier.scopusauthoridYu, RMK=9278574900en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridGiesy, JP=35459135300en_HK

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