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Article: Identification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceae

TitleIdentification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceae
Authors
KeywordsAlga
Cytochrome P450
Diatom
Differential display
Rapid amplification of cDNA ends
Reverse transcription PCR
Skeletonema costatum
Issue Date2003
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPY
Citation
Journal Of Phycology, 2003, v. 39 n. 3, p. 555-560 How to Cite?
AbstractHere we report identification of a 2269-base pair full-length cDNA, CYP97E1, encoding a novel cytochrome P450 protein from the marine diatom Skeletonema costatum. The CYP97E1 protein contains 659 amino acids (Mr 74,200) and is the largest P450 isoform described to date. Our BLAST homology search and parsimony analysis showed that CYP97E1 shared high sequence identity (>40%) and genetic relatedness, respectively, with the CYP97B isoforms from different plant species. CYP97E1 was predicted by PSORT (a protein localization site prediction program) to be a cytosolic protein. Northern hybridization analysis indicated that CYP97E1 expression in S. costatum was not significantly affected by 2,4-dichlorophenol, suggesting that CYP97E1 may not be involved in 2,4-dichlorophenol detoxification in this diatom.
Persistent Identifierhttp://hdl.handle.net/10722/92745
ISSN
2015 Impact Factor: 2.536
2015 SCImago Journal Rankings: 1.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Sen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorMok, HOLen_HK
dc.contributor.authorZhang, ZPen_HK
dc.contributor.authorKong, RYCen_HK
dc.date.accessioned2010-09-17T10:55:55Z-
dc.date.available2010-09-17T10:55:55Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Phycology, 2003, v. 39 n. 3, p. 555-560en_HK
dc.identifier.issn0022-3646en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92745-
dc.description.abstractHere we report identification of a 2269-base pair full-length cDNA, CYP97E1, encoding a novel cytochrome P450 protein from the marine diatom Skeletonema costatum. The CYP97E1 protein contains 659 amino acids (Mr 74,200) and is the largest P450 isoform described to date. Our BLAST homology search and parsimony analysis showed that CYP97E1 shared high sequence identity (>40%) and genetic relatedness, respectively, with the CYP97B isoforms from different plant species. CYP97E1 was predicted by PSORT (a protein localization site prediction program) to be a cytosolic protein. Northern hybridization analysis indicated that CYP97E1 expression in S. costatum was not significantly affected by 2,4-dichlorophenol, suggesting that CYP97E1 may not be involved in 2,4-dichlorophenol detoxification in this diatom.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPYen_HK
dc.relation.ispartofJournal of Phycologyen_HK
dc.subjectAlgaen_HK
dc.subjectCytochrome P450en_HK
dc.subjectDiatomen_HK
dc.subjectDifferential displayen_HK
dc.subjectRapid amplification of cDNA endsen_HK
dc.subjectReverse transcription PCRen_HK
dc.subjectSkeletonema costatumen_HK
dc.titleIdentification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceaeen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1529-8817.2003.02190.xen_HK
dc.identifier.scopuseid_2-s2.0-0038685990en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038685990&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue3en_HK
dc.identifier.spage555en_HK
dc.identifier.epage560en_HK
dc.identifier.isiWOS:000183070000011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYang, S=7406950684en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridMok, HOL=7005842490en_HK
dc.identifier.scopusauthoridZhang, ZP=35228251200en_HK
dc.identifier.scopusauthoridKong, RYC=7005290687en_HK

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