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Article: Induction of hepatic choriogenin mRNA expression in male marine medaka: A highly sensitive biomarker for environmental estrogens

TitleInduction of hepatic choriogenin mRNA expression in male marine medaka: A highly sensitive biomarker for environmental estrogens
Authors
KeywordsChoriogenin
Gene expression
Oryzias javanicus
Promoter
Real-time PCR
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2006, v. 77 n. 4, p. 348-358 How to Cite?
AbstractTeleost choriogenins, precursors of the inner layer subunits of egg envelope, have been recently introduced as sensitive biomarkers for exposure to estrogenic compounds. In this study, two full-length cDNAs-ojChgH and ojChgL which encode the choriogenin H and L forms, respectively, were cloned from the marine medaka, Oryzias javanicus. The deduced protein sequences of ojChgH and ojChgL are highly similar to the corresponding homologues in the freshwater medaka (O. latipes) with identities of 77.2 and 87.6%, respectively. Phylogenetic analysis indicated that ojChgH and ojChgL are members of two different classes of liver-specific ZP-domain containing proteins (ZPB and ZPC, respectively). Computer analysis of ca. 2 kb of the 5′-flanking sequences of ojChgH and ojChgL revealed that both genes contain a number of putative estrogen response elements (EREs) and/or half-site EREs. In vivo mRNA expression patterns of the genes were examined by quantitative real-time RT-PCR. ojChgH is expressed exclusively in the liver while ojChgL is co-expressed in the liver (major) and ovary (minor). Exposure of fish to waterborne 17β-estradiol (E2) at environmentally relevant concentrations (1, 5, 10 and 100 ng/L) resulted in dose-dependent induction of both genes in the liver, with higher sensitivity and magnitude of induction in males than in females. In the male liver, induction of ojChgH is more sensitive to E2 than that of ojChgL and two other estrogen-responsive genes, estrogen receptor α (ojERα) and vitellogenin (ojVTG). The lowest-observed-effect concentration (LOEC) of E2 on induction of hepatic ojChgH mRNA is 1 ng/L. In the ovary, expression of ojChgL is non-responsive to E2 treatment. In conclusion, the present study suggested that induction of hepatic ojChgH mRNA in male fish may be a highly sensitive biomarker for exposure to environmental estrogens. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92741
ISSN
2015 Impact Factor: 3.557
2015 SCImago Journal Rankings: 1.671
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, RMKen_HK
dc.contributor.authorWong, MMLen_HK
dc.contributor.authorKong, RYCen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorCheng, SHen_HK
dc.date.accessioned2010-09-17T10:55:48Z-
dc.date.available2010-09-17T10:55:48Z-
dc.date.issued2006en_HK
dc.identifier.citationAquatic Toxicology, 2006, v. 77 n. 4, p. 348-358en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92741-
dc.description.abstractTeleost choriogenins, precursors of the inner layer subunits of egg envelope, have been recently introduced as sensitive biomarkers for exposure to estrogenic compounds. In this study, two full-length cDNAs-ojChgH and ojChgL which encode the choriogenin H and L forms, respectively, were cloned from the marine medaka, Oryzias javanicus. The deduced protein sequences of ojChgH and ojChgL are highly similar to the corresponding homologues in the freshwater medaka (O. latipes) with identities of 77.2 and 87.6%, respectively. Phylogenetic analysis indicated that ojChgH and ojChgL are members of two different classes of liver-specific ZP-domain containing proteins (ZPB and ZPC, respectively). Computer analysis of ca. 2 kb of the 5′-flanking sequences of ojChgH and ojChgL revealed that both genes contain a number of putative estrogen response elements (EREs) and/or half-site EREs. In vivo mRNA expression patterns of the genes were examined by quantitative real-time RT-PCR. ojChgH is expressed exclusively in the liver while ojChgL is co-expressed in the liver (major) and ovary (minor). Exposure of fish to waterborne 17β-estradiol (E2) at environmentally relevant concentrations (1, 5, 10 and 100 ng/L) resulted in dose-dependent induction of both genes in the liver, with higher sensitivity and magnitude of induction in males than in females. In the male liver, induction of ojChgH is more sensitive to E2 than that of ojChgL and two other estrogen-responsive genes, estrogen receptor α (ojERα) and vitellogenin (ojVTG). The lowest-observed-effect concentration (LOEC) of E2 on induction of hepatic ojChgH mRNA is 1 ng/L. In the ovary, expression of ojChgL is non-responsive to E2 treatment. In conclusion, the present study suggested that induction of hepatic ojChgH mRNA in male fish may be a highly sensitive biomarker for exposure to environmental estrogens. © 2005 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectChoriogeninen_HK
dc.subjectGene expressionen_HK
dc.subjectOryzias javanicusen_HK
dc.subjectPromoteren_HK
dc.subjectReal-time PCRen_HK
dc.titleInduction of hepatic choriogenin mRNA expression in male marine medaka: A highly sensitive biomarker for environmental estrogensen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2006.01.003en_HK
dc.identifier.pmid16464508-
dc.identifier.scopuseid_2-s2.0-33646130865en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646130865&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume77en_HK
dc.identifier.issue4en_HK
dc.identifier.spage348en_HK
dc.identifier.epage358en_HK
dc.identifier.isiWOS:000237813400002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridYu, RMK=9278574900en_HK
dc.identifier.scopusauthoridWong, MML=35390115400en_HK
dc.identifier.scopusauthoridKong, RYC=7005290687en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridCheng, SH=7404684691en_HK
dc.identifier.citeulike567580-

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