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Article: Physiological and cytological responses of the marine diatom Skeletonema costatum to 2,4-dichlorophenol

TitlePhysiological and cytological responses of the marine diatom Skeletonema costatum to 2,4-dichlorophenol
Authors
Keywords2,4-Dichlorophenol
Diatom
Energy charge
Energy reserve
Pigment
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2002, v. 60 n. 1-2, p. 33-41 How to Cite?
AbstractPhysiological and cytological responses of the marine diatom, Skeletonema costatum, to 2,4-dichlorophenol (2,4-DCP) were investigated after exposing algal cultures to 1-6 mg l-1 of 2,4-DCP for 96 h. Growth rate of the diatom was significantly reduced by 2,4-DCP at and above 3.0 mg l-1 while other biological parameters such as photosynthetic and respiration rates, carotenoid and protein content, ATP level and adenylate energy charge were unaffected. Exposure to 6.0 mg l-1 2,4-DCP for 96 h resulted in the total lipid content being increased to 304%, while the RNA/DNA ratio was reduced to 31% of the control values. A parallel study by transmission electron microscopy further confirmed the increase in cellular lipid content, as evidenced by the accumulation of lipid droplets within diatom cells. A slight increase in carbohydrate (+37.8%) and decrease in chlorophyll a (-20.4%) and total chlorophyll c (-14.4%) were also found at 6.0 mg l-1 2,4-DCP. Although 2,4-DCP is known to uncouple oxidative phosphorylation, our results show that energy production was not significantly inhibited at sublethal concentrations of 2,4-DCP. The observed growth inhibition in S. costatum caused by 2,4-DCP was associated with an increase in energy storage and inhibition of protein synthesis, as indicated by a reduction in RNA/DNA ratio. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92734
ISSN
2015 Impact Factor: 3.557
2015 SCImago Journal Rankings: 1.671
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Sen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorKong, RYCen_HK
dc.date.accessioned2010-09-17T10:55:35Z-
dc.date.available2010-09-17T10:55:35Z-
dc.date.issued2002en_HK
dc.identifier.citationAquatic Toxicology, 2002, v. 60 n. 1-2, p. 33-41en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92734-
dc.description.abstractPhysiological and cytological responses of the marine diatom, Skeletonema costatum, to 2,4-dichlorophenol (2,4-DCP) were investigated after exposing algal cultures to 1-6 mg l-1 of 2,4-DCP for 96 h. Growth rate of the diatom was significantly reduced by 2,4-DCP at and above 3.0 mg l-1 while other biological parameters such as photosynthetic and respiration rates, carotenoid and protein content, ATP level and adenylate energy charge were unaffected. Exposure to 6.0 mg l-1 2,4-DCP for 96 h resulted in the total lipid content being increased to 304%, while the RNA/DNA ratio was reduced to 31% of the control values. A parallel study by transmission electron microscopy further confirmed the increase in cellular lipid content, as evidenced by the accumulation of lipid droplets within diatom cells. A slight increase in carbohydrate (+37.8%) and decrease in chlorophyll a (-20.4%) and total chlorophyll c (-14.4%) were also found at 6.0 mg l-1 2,4-DCP. Although 2,4-DCP is known to uncouple oxidative phosphorylation, our results show that energy production was not significantly inhibited at sublethal concentrations of 2,4-DCP. The observed growth inhibition in S. costatum caused by 2,4-DCP was associated with an increase in energy storage and inhibition of protein synthesis, as indicated by a reduction in RNA/DNA ratio. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subject2,4-Dichlorophenolen_HK
dc.subjectDiatomen_HK
dc.subjectEnergy chargeen_HK
dc.subjectEnergy reserveen_HK
dc.subjectPigmenten_HK
dc.titlePhysiological and cytological responses of the marine diatom Skeletonema costatum to 2,4-dichlorophenolen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0166-445X(01)00258-2en_HK
dc.identifier.pmid12204585-
dc.identifier.scopuseid_2-s2.0-0037010203en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037010203&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage33en_HK
dc.identifier.epage41en_HK
dc.identifier.isiWOS:000178066600003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridYang, S=7406950684en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridKong, RYC=7005290687en_HK

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