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Article: Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS

TitleDevelopmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS
Authors
KeywordsApoptosis
Developmental toxicity
Embryo
Gene expression
PFOS
Zebrafish
Issue Date2008
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap
Citation
Toxicology And Applied Pharmacology, 2008, v. 230 n. 1, p. 23-32 How to Cite?
Abstract
Perfluorooctanesulfonate (PFOS) is a persistent organic pollutant, the potential toxicity of which is causing great concern. In the present study, we employed zebrafish embryos to investigate the developmental toxicity of this compound. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to 0.1, 0.5, 1, 3 and 5 mg/L PFOS. Hatching was delayed and hatching rates as well as larval survivorship were significantly reduced after the embryos were exposed to 1, 3 and 5 mg/L PFOS until 132 hpf. The fry displayed gross developmental malformations, including epiboly deformities, hypopigmentation, yolk sac edema, tail and heart malformations and spinal curvature upon exposure to PFOS concentrations of 1 mg/L or greater. Growth (body length) was significantly reduced in the 3 and 5 mg/L PFOS-treated groups. To test whether developmental malformation was mediated via apoptosis, flow cytometry analysis of DNA content, acridine orange staining and TUNEL assay was used. These techniques indicated that more apoptotic cells were present in the PFOS-treated embryos than in the control embryos. Certain genes related to cell apoptosis, p53 and Bax, were both significantly up-regulated upon exposure to all the concentrations tested. In addition, we investigated the effects of PFOS on marker genes related to early thyroid development (hhex and pax8) and genes regulating the balance of androgens and estrogens (cyp19a and cyp19b). For thyroid development, the expression of hhex was significantly up-regulated at all concentrations tested, whereas pax8 expression was significantly up-regulated only upon exposure to lower concentrations of PFOS (0.1, 0.5, 1 mg/L). The expression of cyp19a and of cyp19b was significantly down-regulated at all exposure concentrations. The overall results indicated that zebrafish embryos constitute a reliable model for testing the developmental toxicity of PFOS, and the gene expression patterns in the embryos were able to reveal some potential mechanisms of developmental toxicity. © 2008 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92726
ISSN
2013 Impact Factor: 3.630
ISI Accession Number ID
References

 

Author Affiliations
  1. Institute of Hydrobiology, Chinese Academy of Sciences
  2. City University of Hong Kong
  3. Chinese Academy of Sciences
DC FieldValueLanguage
dc.contributor.authorShi, Xen_HK
dc.contributor.authorDu, Yen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorZhou, Ben_HK
dc.date.accessioned2010-09-17T10:55:22Z-
dc.date.available2010-09-17T10:55:22Z-
dc.date.issued2008en_HK
dc.identifier.citationToxicology And Applied Pharmacology, 2008, v. 230 n. 1, p. 23-32en_HK
dc.identifier.issn0041-008Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92726-
dc.description.abstractPerfluorooctanesulfonate (PFOS) is a persistent organic pollutant, the potential toxicity of which is causing great concern. In the present study, we employed zebrafish embryos to investigate the developmental toxicity of this compound. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to 0.1, 0.5, 1, 3 and 5 mg/L PFOS. Hatching was delayed and hatching rates as well as larval survivorship were significantly reduced after the embryos were exposed to 1, 3 and 5 mg/L PFOS until 132 hpf. The fry displayed gross developmental malformations, including epiboly deformities, hypopigmentation, yolk sac edema, tail and heart malformations and spinal curvature upon exposure to PFOS concentrations of 1 mg/L or greater. Growth (body length) was significantly reduced in the 3 and 5 mg/L PFOS-treated groups. To test whether developmental malformation was mediated via apoptosis, flow cytometry analysis of DNA content, acridine orange staining and TUNEL assay was used. These techniques indicated that more apoptotic cells were present in the PFOS-treated embryos than in the control embryos. Certain genes related to cell apoptosis, p53 and Bax, were both significantly up-regulated upon exposure to all the concentrations tested. In addition, we investigated the effects of PFOS on marker genes related to early thyroid development (hhex and pax8) and genes regulating the balance of androgens and estrogens (cyp19a and cyp19b). For thyroid development, the expression of hhex was significantly up-regulated at all concentrations tested, whereas pax8 expression was significantly up-regulated only upon exposure to lower concentrations of PFOS (0.1, 0.5, 1 mg/L). The expression of cyp19a and of cyp19b was significantly down-regulated at all exposure concentrations. The overall results indicated that zebrafish embryos constitute a reliable model for testing the developmental toxicity of PFOS, and the gene expression patterns in the embryos were able to reveal some potential mechanisms of developmental toxicity. © 2008 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taapen_HK
dc.relation.ispartofToxicology and Applied Pharmacologyen_HK
dc.subjectApoptosisen_HK
dc.subjectDevelopmental toxicityen_HK
dc.subjectEmbryoen_HK
dc.subjectGene expressionen_HK
dc.subjectPFOSen_HK
dc.subjectZebrafishen_HK
dc.titleDevelopmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOSen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.taap.2008.01.043en_HK
dc.identifier.pmid18407306en_HK
dc.identifier.scopuseid_2-s2.0-44649122644en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-44649122644&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume230en_HK
dc.identifier.issue1en_HK
dc.identifier.spage23en_HK
dc.identifier.epage32en_HK
dc.identifier.eissn1096-0333-
dc.identifier.isiWOS:000257098300004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShi, X=16067334700en_HK
dc.identifier.scopusauthoridDu, Y=22979054800en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridZhou, B=7401906781en_HK

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