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Article: Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS
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TitleDevelopmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS
 
AuthorsShi, X1 3
Du, Y1
Lam, PKS2
Wu, RSS2
Zhou, B1
 
KeywordsApoptosis
Developmental toxicity
Embryo
Gene expression
PFOS
Zebrafish
 
Issue Date2008
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap
 
CitationToxicology And Applied Pharmacology, 2008, v. 230 n. 1, p. 23-32 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.taap.2008.01.043
 
AbstractPerfluorooctanesulfonate (PFOS) is a persistent organic pollutant, the potential toxicity of which is causing great concern. In the present study, we employed zebrafish embryos to investigate the developmental toxicity of this compound. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to 0.1, 0.5, 1, 3 and 5 mg/L PFOS. Hatching was delayed and hatching rates as well as larval survivorship were significantly reduced after the embryos were exposed to 1, 3 and 5 mg/L PFOS until 132 hpf. The fry displayed gross developmental malformations, including epiboly deformities, hypopigmentation, yolk sac edema, tail and heart malformations and spinal curvature upon exposure to PFOS concentrations of 1 mg/L or greater. Growth (body length) was significantly reduced in the 3 and 5 mg/L PFOS-treated groups. To test whether developmental malformation was mediated via apoptosis, flow cytometry analysis of DNA content, acridine orange staining and TUNEL assay was used. These techniques indicated that more apoptotic cells were present in the PFOS-treated embryos than in the control embryos. Certain genes related to cell apoptosis, p53 and Bax, were both significantly up-regulated upon exposure to all the concentrations tested. In addition, we investigated the effects of PFOS on marker genes related to early thyroid development (hhex and pax8) and genes regulating the balance of androgens and estrogens (cyp19a and cyp19b). For thyroid development, the expression of hhex was significantly up-regulated at all concentrations tested, whereas pax8 expression was significantly up-regulated only upon exposure to lower concentrations of PFOS (0.1, 0.5, 1 mg/L). The expression of cyp19a and of cyp19b was significantly down-regulated at all exposure concentrations. The overall results indicated that zebrafish embryos constitute a reliable model for testing the developmental toxicity of PFOS, and the gene expression patterns in the embryos were able to reveal some potential mechanisms of developmental toxicity. © 2008 Elsevier Inc. All rights reserved.
 
ISSN0041-008X
2012 Impact Factor: 3.975
2012 SCImago Journal Rankings: 1.328
 
DOIhttp://dx.doi.org/10.1016/j.taap.2008.01.043
 
ISI Accession Number IDWOS:000257098300004
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorShi, X
 
dc.contributor.authorDu, Y
 
dc.contributor.authorLam, PKS
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorZhou, B
 
dc.date.accessioned2010-09-17T10:55:22Z
 
dc.date.available2010-09-17T10:55:22Z
 
dc.date.issued2008
 
dc.description.abstractPerfluorooctanesulfonate (PFOS) is a persistent organic pollutant, the potential toxicity of which is causing great concern. In the present study, we employed zebrafish embryos to investigate the developmental toxicity of this compound. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to 0.1, 0.5, 1, 3 and 5 mg/L PFOS. Hatching was delayed and hatching rates as well as larval survivorship were significantly reduced after the embryos were exposed to 1, 3 and 5 mg/L PFOS until 132 hpf. The fry displayed gross developmental malformations, including epiboly deformities, hypopigmentation, yolk sac edema, tail and heart malformations and spinal curvature upon exposure to PFOS concentrations of 1 mg/L or greater. Growth (body length) was significantly reduced in the 3 and 5 mg/L PFOS-treated groups. To test whether developmental malformation was mediated via apoptosis, flow cytometry analysis of DNA content, acridine orange staining and TUNEL assay was used. These techniques indicated that more apoptotic cells were present in the PFOS-treated embryos than in the control embryos. Certain genes related to cell apoptosis, p53 and Bax, were both significantly up-regulated upon exposure to all the concentrations tested. In addition, we investigated the effects of PFOS on marker genes related to early thyroid development (hhex and pax8) and genes regulating the balance of androgens and estrogens (cyp19a and cyp19b). For thyroid development, the expression of hhex was significantly up-regulated at all concentrations tested, whereas pax8 expression was significantly up-regulated only upon exposure to lower concentrations of PFOS (0.1, 0.5, 1 mg/L). The expression of cyp19a and of cyp19b was significantly down-regulated at all exposure concentrations. The overall results indicated that zebrafish embryos constitute a reliable model for testing the developmental toxicity of PFOS, and the gene expression patterns in the embryos were able to reveal some potential mechanisms of developmental toxicity. © 2008 Elsevier Inc. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationToxicology And Applied Pharmacology, 2008, v. 230 n. 1, p. 23-32 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.taap.2008.01.043
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.taap.2008.01.043
 
dc.identifier.eissn1096-0333
 
dc.identifier.epage32
 
dc.identifier.isiWOS:000257098300004
 
dc.identifier.issn0041-008X
2012 Impact Factor: 3.975
2012 SCImago Journal Rankings: 1.328
 
dc.identifier.issue1
 
dc.identifier.pmid18407306
 
dc.identifier.scopuseid_2-s2.0-44649122644
 
dc.identifier.spage23
 
dc.identifier.urihttp://hdl.handle.net/10722/92726
 
dc.identifier.volume230
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap
 
dc.publisher.placeUnited States
 
dc.relation.ispartofToxicology and Applied Pharmacology
 
dc.relation.referencesReferences in Scopus
 
dc.subjectApoptosis
 
dc.subjectDevelopmental toxicity
 
dc.subjectEmbryo
 
dc.subjectGene expression
 
dc.subjectPFOS
 
dc.subjectZebrafish
 
dc.titleDevelopmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS
 
dc.typeArticle
 
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<description.abstract>Perfluorooctanesulfonate (PFOS) is a persistent organic pollutant, the potential toxicity of which is causing great concern. In the present study, we employed zebrafish embryos to investigate the developmental toxicity of this compound. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to 0.1, 0.5, 1, 3 and 5&#160;mg/L PFOS. Hatching was delayed and hatching rates as well as larval survivorship were significantly reduced after the embryos were exposed to 1, 3 and 5&#160;mg/L PFOS until 132&#160;hpf. The fry displayed gross developmental malformations, including epiboly deformities, hypopigmentation, yolk sac edema, tail and heart malformations and spinal curvature upon exposure to PFOS concentrations of 1&#160;mg/L or greater. Growth (body length) was significantly reduced in the 3 and 5&#160;mg/L PFOS-treated groups. To test whether developmental malformation was mediated via apoptosis, flow cytometry analysis of DNA content, acridine orange staining and TUNEL assay was used. These techniques indicated that more apoptotic cells were present in the PFOS-treated embryos than in the control embryos. Certain genes related to cell apoptosis, p53 and Bax, were both significantly up-regulated upon exposure to all the concentrations tested. In addition, we investigated the effects of PFOS on marker genes related to early thyroid development (hhex and pax8) and genes regulating the balance of androgens and estrogens (cyp19a and cyp19b). For thyroid development, the expression of hhex was significantly up-regulated at all concentrations tested, whereas pax8 expression was significantly up-regulated only upon exposure to lower concentrations of PFOS (0.1, 0.5, 1&#160;mg/L). The expression of cyp19a and of cyp19b was significantly down-regulated at all exposure concentrations. The overall results indicated that zebrafish embryos constitute a reliable model for testing the developmental toxicity of PFOS, and the gene expression patterns in the embryos were able to reveal some potential mechanisms of developmental toxicity. &#169; 2008 Elsevier Inc. All rights reserved.</description.abstract>
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Author Affiliations
  1. Institute of Hydrobiology, Chinese Academy of Sciences
  2. City University of Hong Kong
  3. Chinese Academy of Sciences