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Article: Ultraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina

TitleUltraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina
Authors
KeywordsFlow cytometry
Membrane integrity
Mitochondrial function
Sea urchin
UVA
UVB
Issue Date2005
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv
Citation
Ecotoxicology And Environmental Safety, 2005, v. 61 n. 1, p. 53-59 How to Cite?
AbstractEffects of ultraviolet A (UVA) and ultraviolet B (UVB) on mitochondrial function and membrane integrity of sea urchin sperm were investigated using flow cytometry and fluorescent probes. Both UVA and UVB impaired sperm mitochondrial function in a dose-dependent manner. Covariance analysis further showed that the slopes of change in mitochondrial function in relation to UVA and UVB were significantly different, suggesting that the modes of action were different. UVA did not affect membrane integrity, while membrane integrity showed a linear reduction with increasing UVB doses. Sperm mitochondria function showed significant positive correlations with sperm motility and subsequent fertilization success. Overall, our results showed that both UVA and UVB could decrease sperm motility and fertilization success through impairment of mitochondrial function, whereas UVB alone could cause additional damage through impairing the functional integrity of sperm membrane. Mitochondrial function of sperm may also offer a reliable ecotoxicological biomarker for predicting fertilization success in urchins. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92721
ISSN
2015 Impact Factor: 3.13
2015 SCImago Journal Rankings: 1.229
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, XYen_HK
dc.contributor.authorWu, RSSen_HK
dc.date.accessioned2010-09-17T10:55:13Z-
dc.date.available2010-09-17T10:55:13Z-
dc.date.issued2005en_HK
dc.identifier.citationEcotoxicology And Environmental Safety, 2005, v. 61 n. 1, p. 53-59en_HK
dc.identifier.issn0147-6513en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92721-
dc.description.abstractEffects of ultraviolet A (UVA) and ultraviolet B (UVB) on mitochondrial function and membrane integrity of sea urchin sperm were investigated using flow cytometry and fluorescent probes. Both UVA and UVB impaired sperm mitochondrial function in a dose-dependent manner. Covariance analysis further showed that the slopes of change in mitochondrial function in relation to UVA and UVB were significantly different, suggesting that the modes of action were different. UVA did not affect membrane integrity, while membrane integrity showed a linear reduction with increasing UVB doses. Sperm mitochondria function showed significant positive correlations with sperm motility and subsequent fertilization success. Overall, our results showed that both UVA and UVB could decrease sperm motility and fertilization success through impairment of mitochondrial function, whereas UVB alone could cause additional damage through impairing the functional integrity of sperm membrane. Mitochondrial function of sperm may also offer a reliable ecotoxicological biomarker for predicting fertilization success in urchins. © 2005 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenven_HK
dc.relation.ispartofEcotoxicology and Environmental Safetyen_HK
dc.subjectFlow cytometryen_HK
dc.subjectMembrane integrityen_HK
dc.subjectMitochondrial functionen_HK
dc.subjectSea urchinen_HK
dc.subjectUVAen_HK
dc.subjectUVBen_HK
dc.titleUltraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispinaen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ecoenv.2004.12.012en_HK
dc.identifier.pmid15814310-
dc.identifier.scopuseid_2-s2.0-16344389131en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-16344389131&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume61en_HK
dc.identifier.issue1en_HK
dc.identifier.spage53en_HK
dc.identifier.epage59en_HK
dc.identifier.isiWOS:000228623100005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLu, XY=37062757600en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK

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