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Article: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus

TitleIsolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus
Authors
KeywordsCtenopharyngodon idellus
Glucose transporter
Grass carp
Hypoxia
Split codon
Issue Date2003
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB
Citation
European Journal Of Biochemistry, 2003, v. 270 n. 14, p. 3010-3017 How to Cite?
AbstractGlucose transporters (GLUTs) have been implicated in adaptive and survival responses to hypoxic stress in mammals. In fish, the expression and regulation of GLUT in relation to hypoxia remains unexplored. Here we describe the identification of a hypoxia-responsive glucose transporter gene (gcGLUT) and the corresponding full-length cDNA from the grass carp. The gene spans ≈ 11 kb of genomic sequence and consists of 12 exons and 11 introns, and an open reading frame (ORF) of 1599 bp encoding a polypeptide of 533 amino acids, with a predicted molecular mass of ≈ 57 kDa and a pI of 8.34. BLASTX analysis showed that the ORF shared high sequence identity with the GLUT1 (57-59%), GLUT3 (59-60%) and GLUT4 (55-59%) proteins from different vertebrates. Comparative analysis of GLUT genomic structures showed that the arrangement of exons and position of split codons are highly conserved amongst members of the class I GLUTs suggesting that these genes share a common ancestor. Phylogenetic analysis indicated that gcGLUT is most closely related to the GLUT3 proteins. Northern blot analysis showed that the 3.1-kb gcGLUT transcript was most abundantly expressed and responsive to hypoxia in kidney. Up-regulated expression by hypoxia was also evident in eye and gill, but differential patterns of expression were observed. Low expression levels detected in brain, heart, liver and muscle were not responsive to hypoxic stress.
Persistent Identifierhttp://hdl.handle.net/10722/92713
ISSN
2006 Impact Factor: 3.579
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Zen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorMok, HOLen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorPoon, WWLen_HK
dc.contributor.authorCheng, SHen_HK
dc.contributor.authorKong, RYCen_HK
dc.date.accessioned2010-09-17T10:54:59Z-
dc.date.available2010-09-17T10:54:59Z-
dc.date.issued2003en_HK
dc.identifier.citationEuropean Journal Of Biochemistry, 2003, v. 270 n. 14, p. 3010-3017en_HK
dc.identifier.issn0014-2956en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92713-
dc.description.abstractGlucose transporters (GLUTs) have been implicated in adaptive and survival responses to hypoxic stress in mammals. In fish, the expression and regulation of GLUT in relation to hypoxia remains unexplored. Here we describe the identification of a hypoxia-responsive glucose transporter gene (gcGLUT) and the corresponding full-length cDNA from the grass carp. The gene spans ≈ 11 kb of genomic sequence and consists of 12 exons and 11 introns, and an open reading frame (ORF) of 1599 bp encoding a polypeptide of 533 amino acids, with a predicted molecular mass of ≈ 57 kDa and a pI of 8.34. BLASTX analysis showed that the ORF shared high sequence identity with the GLUT1 (57-59%), GLUT3 (59-60%) and GLUT4 (55-59%) proteins from different vertebrates. Comparative analysis of GLUT genomic structures showed that the arrangement of exons and position of split codons are highly conserved amongst members of the class I GLUTs suggesting that these genes share a common ancestor. Phylogenetic analysis indicated that gcGLUT is most closely related to the GLUT3 proteins. Northern blot analysis showed that the 3.1-kb gcGLUT transcript was most abundantly expressed and responsive to hypoxia in kidney. Up-regulated expression by hypoxia was also evident in eye and gill, but differential patterns of expression were observed. Low expression levels detected in brain, heart, liver and muscle were not responsive to hypoxic stress.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJBen_HK
dc.relation.ispartofEuropean Journal of Biochemistryen_HK
dc.subjectCtenopharyngodon idellusen_HK
dc.subjectGlucose transporteren_HK
dc.subjectGrass carpen_HK
dc.subjectHypoxiaen_HK
dc.subjectSplit codonen_HK
dc.titleIsolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellusen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1432-1033.2003.03678.xen_HK
dc.identifier.pmid12846834-
dc.identifier.scopuseid_2-s2.0-0038375267en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038375267&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume270en_HK
dc.identifier.issue14en_HK
dc.identifier.spage3010en_HK
dc.identifier.epage3017en_HK
dc.identifier.isiWOS:000183959900011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhang, Z=35228251200en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridMok, HOL=7005842490en_HK
dc.identifier.scopusauthoridWang, Y=8424277700en_HK
dc.identifier.scopusauthoridPoon, WWL=36892190700en_HK
dc.identifier.scopusauthoridCheng, SH=7404684691en_HK
dc.identifier.scopusauthoridKong, RYC=7005290687en_HK

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