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Article: Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis)

TitleApplication of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis)
Authors
KeywordsBenzo[a]pyrene
Comet assay
DNA damage
Haemocyte
Micronucleus
Perna viridis
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2004, v. 66 n. 4, p. 381-392 How to Cite?
AbstractGreen-lipped mussels (Perna viridis) were exposed to water-borne benzo[a]pyrene (B[a]P) at nominal concentrations of 0, 0.3, 3 and 30μgl -1 for up to 12 days, and both the relative levels of DNA strand breaks (assessed using an alkaline comet assay) and the proportion of micronucleus (MN) formation were monitored in mussel haemocytes at days 0, 1, 3, 6 and 12. The results of the comet assay indicated that an increase in the proportion of strand breaks occurred generally with increasing B[a]P concentration, but a significant decrease in the levels of DNA damage was observed after exposure for 12 days at all concentrations tested, suggesting that the patterns of changes in the levels of DNA strand breakage can be explained by the threshold dependent DNA repair theory. Moreover, the relatively slow development and recovery of the DNA damage response in mussel haemocytes in comparison with previous findings utilizing P. viridis hepatopancreas suggests that the response of DNA alteration upon exposure to B[a]P may be tissue-specific in this species. Monitoring the frequency of micronucleus development in mussel haemocytes indicated both dose- and time-response relationships within the exposure period. Furthermore, the levels of DNA strand breakage correlated well with the levels of micronucleus induction, suggesting a possible cause and effect relationship between the two damage types. We suggest that DNA strand breakage and micronucleus formation in mussel haemocytes can potentially be used as convenient biomarkers of exposure to genotoxicants in the marine environment. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92711
ISSN
2014 Impact Factor: 3.451
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSiu, WHLen_HK
dc.contributor.authorCao, Jen_HK
dc.contributor.authorJack, RWen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorRichardson, BJen_HK
dc.contributor.authorXu, Len_HK
dc.contributor.authorLam, PKSen_HK
dc.date.accessioned2010-09-17T10:54:55Z-
dc.date.available2010-09-17T10:54:55Z-
dc.date.issued2004en_HK
dc.identifier.citationAquatic Toxicology, 2004, v. 66 n. 4, p. 381-392en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92711-
dc.description.abstractGreen-lipped mussels (Perna viridis) were exposed to water-borne benzo[a]pyrene (B[a]P) at nominal concentrations of 0, 0.3, 3 and 30μgl -1 for up to 12 days, and both the relative levels of DNA strand breaks (assessed using an alkaline comet assay) and the proportion of micronucleus (MN) formation were monitored in mussel haemocytes at days 0, 1, 3, 6 and 12. The results of the comet assay indicated that an increase in the proportion of strand breaks occurred generally with increasing B[a]P concentration, but a significant decrease in the levels of DNA damage was observed after exposure for 12 days at all concentrations tested, suggesting that the patterns of changes in the levels of DNA strand breakage can be explained by the threshold dependent DNA repair theory. Moreover, the relatively slow development and recovery of the DNA damage response in mussel haemocytes in comparison with previous findings utilizing P. viridis hepatopancreas suggests that the response of DNA alteration upon exposure to B[a]P may be tissue-specific in this species. Monitoring the frequency of micronucleus development in mussel haemocytes indicated both dose- and time-response relationships within the exposure period. Furthermore, the levels of DNA strand breakage correlated well with the levels of micronucleus induction, suggesting a possible cause and effect relationship between the two damage types. We suggest that DNA strand breakage and micronucleus formation in mussel haemocytes can potentially be used as convenient biomarkers of exposure to genotoxicants in the marine environment. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectBenzo[a]pyreneen_HK
dc.subjectComet assayen_HK
dc.subjectDNA damageen_HK
dc.subjectHaemocyteen_HK
dc.subjectMicronucleusen_HK
dc.subjectPerna viridisen_HK
dc.titleApplication of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis)en_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2003.10.006en_HK
dc.identifier.pmid15168946en_HK
dc.identifier.scopuseid_2-s2.0-1042266252en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1042266252&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume66en_HK
dc.identifier.issue4en_HK
dc.identifier.spage381en_HK
dc.identifier.epage392en_HK
dc.identifier.isiWOS:000189228100004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridSiu, WHL=9272174300en_HK
dc.identifier.scopusauthoridCao, J=26643166500en_HK
dc.identifier.scopusauthoridJack, RW=36957448400en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridRichardson, BJ=7202395817en_HK
dc.identifier.scopusauthoridXu, L=7404745586en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK

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