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Article: Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis)
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TitleApplication of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis)
 
AuthorsSiu, WHL2
Cao, J4
Jack, RW2 3
Wu, RSS2
Richardson, BJ2
Xu, L1
Lam, PKS2
 
KeywordsBenzo[a]pyrene
Comet assay
DNA damage
Haemocyte
Micronucleus
Perna viridis
 
Issue Date2004
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
 
CitationAquatic Toxicology, 2004, v. 66 n. 4, p. 381-392 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2003.10.006
 
AbstractGreen-lipped mussels (Perna viridis) were exposed to water-borne benzo[a]pyrene (B[a]P) at nominal concentrations of 0, 0.3, 3 and 30μgl -1 for up to 12 days, and both the relative levels of DNA strand breaks (assessed using an alkaline comet assay) and the proportion of micronucleus (MN) formation were monitored in mussel haemocytes at days 0, 1, 3, 6 and 12. The results of the comet assay indicated that an increase in the proportion of strand breaks occurred generally with increasing B[a]P concentration, but a significant decrease in the levels of DNA damage was observed after exposure for 12 days at all concentrations tested, suggesting that the patterns of changes in the levels of DNA strand breakage can be explained by the threshold dependent DNA repair theory. Moreover, the relatively slow development and recovery of the DNA damage response in mussel haemocytes in comparison with previous findings utilizing P. viridis hepatopancreas suggests that the response of DNA alteration upon exposure to B[a]P may be tissue-specific in this species. Monitoring the frequency of micronucleus development in mussel haemocytes indicated both dose- and time-response relationships within the exposure period. Furthermore, the levels of DNA strand breakage correlated well with the levels of micronucleus induction, suggesting a possible cause and effect relationship between the two damage types. We suggest that DNA strand breakage and micronucleus formation in mussel haemocytes can potentially be used as convenient biomarkers of exposure to genotoxicants in the marine environment. © 2003 Elsevier B.V. All rights reserved.
 
ISSN0166-445X
2013 Impact Factor: 3.513
 
DOIhttp://dx.doi.org/10.1016/j.aquatox.2003.10.006
 
ISI Accession Number IDWOS:000189228100004
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSiu, WHL
 
dc.contributor.authorCao, J
 
dc.contributor.authorJack, RW
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorRichardson, BJ
 
dc.contributor.authorXu, L
 
dc.contributor.authorLam, PKS
 
dc.date.accessioned2010-09-17T10:54:55Z
 
dc.date.available2010-09-17T10:54:55Z
 
dc.date.issued2004
 
dc.description.abstractGreen-lipped mussels (Perna viridis) were exposed to water-borne benzo[a]pyrene (B[a]P) at nominal concentrations of 0, 0.3, 3 and 30μgl -1 for up to 12 days, and both the relative levels of DNA strand breaks (assessed using an alkaline comet assay) and the proportion of micronucleus (MN) formation were monitored in mussel haemocytes at days 0, 1, 3, 6 and 12. The results of the comet assay indicated that an increase in the proportion of strand breaks occurred generally with increasing B[a]P concentration, but a significant decrease in the levels of DNA damage was observed after exposure for 12 days at all concentrations tested, suggesting that the patterns of changes in the levels of DNA strand breakage can be explained by the threshold dependent DNA repair theory. Moreover, the relatively slow development and recovery of the DNA damage response in mussel haemocytes in comparison with previous findings utilizing P. viridis hepatopancreas suggests that the response of DNA alteration upon exposure to B[a]P may be tissue-specific in this species. Monitoring the frequency of micronucleus development in mussel haemocytes indicated both dose- and time-response relationships within the exposure period. Furthermore, the levels of DNA strand breakage correlated well with the levels of micronucleus induction, suggesting a possible cause and effect relationship between the two damage types. We suggest that DNA strand breakage and micronucleus formation in mussel haemocytes can potentially be used as convenient biomarkers of exposure to genotoxicants in the marine environment. © 2003 Elsevier B.V. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationAquatic Toxicology, 2004, v. 66 n. 4, p. 381-392 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2003.10.006
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.aquatox.2003.10.006
 
dc.identifier.epage392
 
dc.identifier.isiWOS:000189228100004
 
dc.identifier.issn0166-445X
2013 Impact Factor: 3.513
 
dc.identifier.issue4
 
dc.identifier.pmid15168946
 
dc.identifier.scopuseid_2-s2.0-1042266252
 
dc.identifier.spage381
 
dc.identifier.urihttp://hdl.handle.net/10722/92711
 
dc.identifier.volume66
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofAquatic Toxicology
 
dc.relation.referencesReferences in Scopus
 
dc.subjectBenzo[a]pyrene
 
dc.subjectComet assay
 
dc.subjectDNA damage
 
dc.subjectHaemocyte
 
dc.subjectMicronucleus
 
dc.subjectPerna viridis
 
dc.titleApplication of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis)
 
dc.typeArticle
 
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<contributor.author>Richardson, BJ</contributor.author>
<contributor.author>Xu, L</contributor.author>
<contributor.author>Lam, PKS</contributor.author>
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Author Affiliations
  1. Zhejiang University
  2. City University of Hong Kong
  3. University of Otago
  4. Third Military Medical University