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Article: Real-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka

TitleReal-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka
Authors
KeywordsEndocrine
Expression profile
Fecundity
Fish
Steroidogenesis
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2008, v. 88 n. 3, p. 173-182 How to Cite?
Abstract
This paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17α-ethinylestradiol (EE2) and the anabolic androgen, 17β-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-α expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92706
ISSN
2013 Impact Factor: 3.513
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorHecker, Men_HK
dc.contributor.authorPark, JWen_HK
dc.contributor.authorTompsett, ARen_HK
dc.contributor.authorNewsted, Jen_HK
dc.contributor.authorNakayama, Ken_HK
dc.contributor.authorJones, PDen_HK
dc.contributor.authorAu, Den_HK
dc.contributor.authorKong, Ren_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorGiesy, JPen_HK
dc.date.accessioned2010-09-17T10:54:47Z-
dc.date.available2010-09-17T10:54:47Z-
dc.date.issued2008en_HK
dc.identifier.citationAquatic Toxicology, 2008, v. 88 n. 3, p. 173-182en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92706-
dc.description.abstractThis paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17α-ethinylestradiol (EE2) and the anabolic androgen, 17β-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-α expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectEndocrineen_HK
dc.subjectExpression profileen_HK
dc.subjectFecundityen_HK
dc.subjectFishen_HK
dc.subjectSteroidogenesisen_HK
dc.titleReal-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medakaen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2008.04.009en_HK
dc.identifier.pmid18534694en_HK
dc.identifier.scopuseid_2-s2.0-46749127694en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-46749127694&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume88en_HK
dc.identifier.issue3en_HK
dc.identifier.spage173en_HK
dc.identifier.epage182en_HK
dc.identifier.isiWOS:000258124500003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhang, X=8606600100en_HK
dc.identifier.scopusauthoridHecker, M=35247848500en_HK
dc.identifier.scopusauthoridPark, JW=8414402800en_HK
dc.identifier.scopusauthoridTompsett, AR=12244509300en_HK
dc.identifier.scopusauthoridNewsted, J=6603677236en_HK
dc.identifier.scopusauthoridNakayama, K=34882054500en_HK
dc.identifier.scopusauthoridJones, PD=34771015600en_HK
dc.identifier.scopusauthoridAu, D=7004909228en_HK
dc.identifier.scopusauthoridKong, R=7005290687en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridGiesy, JP=35459135300en_HK

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