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Article: Real-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka

TitleReal-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka
Authors
KeywordsEndocrine
Expression profile
Fecundity
Fish
Steroidogenesis
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2008, v. 88 n. 3, p. 173-182 How to Cite?
Abstract
This paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17α-ethinylestradiol (EE2) and the anabolic androgen, 17β-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-α expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92706
ISSN
2013 Impact Factor: 3.513
ISI Accession Number ID
References

 

Author Affiliations
  1. University of Saskatchewan
  2. Michigan State University
  3. ENTRIX Inc.
  4. City University of Hong Kong
  5. Ehime University
DC FieldValueLanguage
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorHecker, Men_HK
dc.contributor.authorPark, JWen_HK
dc.contributor.authorTompsett, ARen_HK
dc.contributor.authorNewsted, Jen_HK
dc.contributor.authorNakayama, Ken_HK
dc.contributor.authorJones, PDen_HK
dc.contributor.authorAu, Den_HK
dc.contributor.authorKong, Ren_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorGiesy, JPen_HK
dc.date.accessioned2010-09-17T10:54:47Z-
dc.date.available2010-09-17T10:54:47Z-
dc.date.issued2008en_HK
dc.identifier.citationAquatic Toxicology, 2008, v. 88 n. 3, p. 173-182en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92706-
dc.description.abstractThis paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17α-ethinylestradiol (EE2) and the anabolic androgen, 17β-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-α expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectEndocrineen_HK
dc.subjectExpression profileen_HK
dc.subjectFecundityen_HK
dc.subjectFishen_HK
dc.subjectSteroidogenesisen_HK
dc.titleReal-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medakaen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2008.04.009en_HK
dc.identifier.pmid18534694en_HK
dc.identifier.scopuseid_2-s2.0-46749127694en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-46749127694&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume88en_HK
dc.identifier.issue3en_HK
dc.identifier.spage173en_HK
dc.identifier.epage182en_HK
dc.identifier.isiWOS:000258124500003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhang, X=8606600100en_HK
dc.identifier.scopusauthoridHecker, M=35247848500en_HK
dc.identifier.scopusauthoridPark, JW=8414402800en_HK
dc.identifier.scopusauthoridTompsett, AR=12244509300en_HK
dc.identifier.scopusauthoridNewsted, J=6603677236en_HK
dc.identifier.scopusauthoridNakayama, K=34882054500en_HK
dc.identifier.scopusauthoridJones, PD=34771015600en_HK
dc.identifier.scopusauthoridAu, D=7004909228en_HK
dc.identifier.scopusauthoridKong, R=7005290687en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridGiesy, JP=35459135300en_HK

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