Article: Real-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka

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TitleReal-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka
AuthorsZhang, X2
Hecker, M1 2 3
Park, JW2
Tompsett, AR2
Newsted, J2 3
Nakayama, K5
Jones, PD1 2
Au, D4
Kong, R4
Wu, RSS4
Giesy, JP1 2 4
KeywordsEndocrine
Expression profile
Fecundity
Fish
Steroidogenesis
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
CitationAquatic Toxicology, 2008, v. 88 n. 3, p. 173-182 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2008.04.009
AbstractThis paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17α-ethinylestradiol (EE2) and the anabolic androgen, 17β-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-α expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved.
ISSN0166-445X
2011 Impact Factor: 3.761
2011 SCImago Journal Rankings: 0.149
DOIhttp://dx.doi.org/10.1016/j.aquatox.2008.04.009
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorZhang, X
dc.contributor.authorHecker, M
dc.contributor.authorPark, JW
dc.contributor.authorTompsett, AR
dc.contributor.authorNewsted, J
dc.contributor.authorNakayama, K
dc.contributor.authorJones, PD
dc.contributor.authorAu, D
dc.contributor.authorKong, R
dc.contributor.authorWu, RSS
dc.contributor.authorGiesy, JP
dc.date.accessioned2010-09-17T10:54:47Z
dc.date.available2010-09-17T10:54:47Z
dc.date.issued2008
dc.description.abstractThis paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17α-ethinylestradiol (EE2) and the anabolic androgen, 17β-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-α expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationAquatic Toxicology, 2008, v. 88 n. 3, p. 173-182 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2008.04.009
dc.identifier.doihttp://dx.doi.org/10.1016/j.aquatox.2008.04.009
dc.identifier.epage182
dc.identifier.isiWOS:000258124500003
dc.identifier.issn0166-445X
2011 Impact Factor: 3.761
2011 SCImago Journal Rankings: 0.149
dc.identifier.issue3
dc.identifier.pmid18534694
dc.identifier.scopuseid_2-s2.0-46749127694
dc.identifier.spage173
dc.identifier.urihttp://hdl.handle.net/10722/92706
dc.identifier.volume88
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
dc.publisher.placeNetherlands
dc.relation.ispartofAquatic Toxicology
dc.relation.referencesReferences in Scopus
dc.subjectEndocrine
dc.subjectExpression profile
dc.subjectFecundity
dc.subjectFish
dc.subjectSteroidogenesis
dc.titleReal-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka
dc.typeArticle
Author Affiliations
  1. University of Saskatchewan
  2. Michigan State University
  3. ENTRIX Inc.
  4. City University of Hong Kong
  5. Ehime University