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Article: Real-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka
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TitleReal-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka
 
AuthorsZhang, X2
Hecker, M3 2 1
Park, JW2
Tompsett, AR2
Newsted, J2 3
Nakayama, K5
Jones, PD2 1
Au, D4
Kong, R4
Wu, RSS4
Giesy, JP4 2 1
 
KeywordsEndocrine
Expression profile
Fecundity
Fish
Steroidogenesis
 
Issue Date2008
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
 
CitationAquatic Toxicology, 2008, v. 88 n. 3, p. 173-182 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2008.04.009
 
AbstractThis paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17α-ethinylestradiol (EE2) and the anabolic androgen, 17β-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-α expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved.
 
ISSN0166-445X
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.615
 
DOIhttp://dx.doi.org/10.1016/j.aquatox.2008.04.009
 
ISI Accession Number IDWOS:000258124500003
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhang, X
 
dc.contributor.authorHecker, M
 
dc.contributor.authorPark, JW
 
dc.contributor.authorTompsett, AR
 
dc.contributor.authorNewsted, J
 
dc.contributor.authorNakayama, K
 
dc.contributor.authorJones, PD
 
dc.contributor.authorAu, D
 
dc.contributor.authorKong, R
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorGiesy, JP
 
dc.date.accessioned2010-09-17T10:54:47Z
 
dc.date.available2010-09-17T10:54:47Z
 
dc.date.issued2008
 
dc.description.abstractThis paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17α-ethinylestradiol (EE2) and the anabolic androgen, 17β-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-α expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationAquatic Toxicology, 2008, v. 88 n. 3, p. 173-182 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2008.04.009
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.aquatox.2008.04.009
 
dc.identifier.epage182
 
dc.identifier.isiWOS:000258124500003
 
dc.identifier.issn0166-445X
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.615
 
dc.identifier.issue3
 
dc.identifier.pmid18534694
 
dc.identifier.scopuseid_2-s2.0-46749127694
 
dc.identifier.spage173
 
dc.identifier.urihttp://hdl.handle.net/10722/92706
 
dc.identifier.volume88
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofAquatic Toxicology
 
dc.relation.referencesReferences in Scopus
 
dc.subjectEndocrine
 
dc.subjectExpression profile
 
dc.subjectFecundity
 
dc.subjectFish
 
dc.subjectSteroidogenesis
 
dc.titleReal-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka
 
dc.typeArticle
 
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<contributor.author>Nakayama, K</contributor.author>
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<contributor.author>Au, D</contributor.author>
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Author Affiliations
  1. University of Saskatchewan
  2. Michigan State University
  3. ENTRIX Inc.
  4. City University of Hong Kong
  5. Ehime University