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Article: Primary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia

TitlePrimary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia
Authors
KeywordsEROD
Gill epithelia
Hepatocytes
Primary cultured cells
Tilapia
Toxicity
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2006, v. 80 n. 2, p. 109-118 How to Cite?
AbstractIn an effort to develop cultured cell models for toxicity screening and environmental biomonitoring, we compared primary cultured gill epithelia and hepatocytes from freshwater tilapia (Oreochromis niloticus) to assess their sensitivity to AhR agonist toxicants. Epithelia were cultured on permeable supports (terephthalate membranes, "filters") and bathed on the apical with waterborne toxicants (pseudo in vivo asymmetrical culture conditions). Hepatocytes were cultured in multi-well plates and exposed to toxicants in culture medium. Cytochrome P4501A (measured as 7-Ethoxyresorufin-O-deethylase, EROD) was selected as a biomarker. For cultured gill epithelia, the integrity of the epithelia remained unchanged on exposure to model toxicants, such as 1,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), benzo(a)pyrene B[a]P, polychlorinated biphenyl (PCB) mixture (Aroclor 1254), and polybrominated diphenyl ether (PBDE) mixture (DE71). A good concentration-dependent response of EROD activity was clearly observed in both cultured gill epithelia and hepatocytes. The time-course response of EROD was measured as early as 3 h, and was maximal after 6 h of exposure to TCDD, B[a]P and Aroclor 1254. The estimated 6 h EC50 for TCDD, B[a]P, and Aroclor 1254 was 1.2 × 10-9, 5.7 × 10-8 and 6.6 × 10-6 M. For the cultured hepatocytes, time-course study showed that a significant induction of EROD took place at 18 h, and the maximal induction of EROD was observed at 24 h after exposure. The estimated 24 h EC50 for TCDD, B[a]P, and Aroclor 1254 was 1.4 × 10-9, 8.1 × 10-8 and 7.3 × 10-6 M. There was no induction or inhibition of EROD in DE71 exposure to both gill epithelia and hepatocytes. The results show that cultured gill epithelia more rapidly induce EROD and are slightly more sensitive than cultured hepatocytes, and could be used as a rapid and sensitive tool for screening chemicals and monitoring environmental AhR agonist toxicants. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92701
ISSN
2015 Impact Factor: 3.557
2015 SCImago Journal Rankings: 1.671
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Ben_HK
dc.contributor.authorLiu, Cen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWu, RSSen_HK
dc.date.accessioned2010-09-17T10:54:37Z-
dc.date.available2010-09-17T10:54:37Z-
dc.date.issued2006en_HK
dc.identifier.citationAquatic Toxicology, 2006, v. 80 n. 2, p. 109-118en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92701-
dc.description.abstractIn an effort to develop cultured cell models for toxicity screening and environmental biomonitoring, we compared primary cultured gill epithelia and hepatocytes from freshwater tilapia (Oreochromis niloticus) to assess their sensitivity to AhR agonist toxicants. Epithelia were cultured on permeable supports (terephthalate membranes, "filters") and bathed on the apical with waterborne toxicants (pseudo in vivo asymmetrical culture conditions). Hepatocytes were cultured in multi-well plates and exposed to toxicants in culture medium. Cytochrome P4501A (measured as 7-Ethoxyresorufin-O-deethylase, EROD) was selected as a biomarker. For cultured gill epithelia, the integrity of the epithelia remained unchanged on exposure to model toxicants, such as 1,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), benzo(a)pyrene B[a]P, polychlorinated biphenyl (PCB) mixture (Aroclor 1254), and polybrominated diphenyl ether (PBDE) mixture (DE71). A good concentration-dependent response of EROD activity was clearly observed in both cultured gill epithelia and hepatocytes. The time-course response of EROD was measured as early as 3 h, and was maximal after 6 h of exposure to TCDD, B[a]P and Aroclor 1254. The estimated 6 h EC50 for TCDD, B[a]P, and Aroclor 1254 was 1.2 × 10-9, 5.7 × 10-8 and 6.6 × 10-6 M. For the cultured hepatocytes, time-course study showed that a significant induction of EROD took place at 18 h, and the maximal induction of EROD was observed at 24 h after exposure. The estimated 24 h EC50 for TCDD, B[a]P, and Aroclor 1254 was 1.4 × 10-9, 8.1 × 10-8 and 7.3 × 10-6 M. There was no induction or inhibition of EROD in DE71 exposure to both gill epithelia and hepatocytes. The results show that cultured gill epithelia more rapidly induce EROD and are slightly more sensitive than cultured hepatocytes, and could be used as a rapid and sensitive tool for screening chemicals and monitoring environmental AhR agonist toxicants. © 2006 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectERODen_HK
dc.subjectGill epitheliaen_HK
dc.subjectHepatocytesen_HK
dc.subjectPrimary cultured cellsen_HK
dc.subjectTilapiaen_HK
dc.subjectToxicityen_HK
dc.titlePrimary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epitheliaen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2006.07.021en_HK
dc.identifier.pmid16959333-
dc.identifier.scopuseid_2-s2.0-33750040544en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750040544&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume80en_HK
dc.identifier.issue2en_HK
dc.identifier.spage109en_HK
dc.identifier.epage118en_HK
dc.identifier.isiWOS:000242064100002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhou, B=7401906781en_HK
dc.identifier.scopusauthoridLiu, C=8318316300en_HK
dc.identifier.scopusauthoridWang, J=8941425500en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK

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