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Article: Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp

TitleMolecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp
Authors
KeywordsCITED
Co-transactivator
Ctenopharyngodon idellus
Gene regulation
Grass carp
Hypoxia
RACE PCR
Issue Date2003
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cbpb
Citation
Comparative Biochemistry And Physiology - B Biochemistry And Molecular Biology, 2003, v. 136 n. 2, p. 163-172 How to Cite?
AbstractWe have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92700
ISSN
2015 Impact Factor: 1.651
2015 SCImago Journal Rankings: 0.709
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, PKSen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorZhang, ZPen_HK
dc.contributor.authorMok, HOLen_HK
dc.contributor.authorRandall, DJen_HK
dc.contributor.authorKong, RYCen_HK
dc.date.accessioned2010-09-17T10:54:35Z-
dc.date.available2010-09-17T10:54:35Z-
dc.date.issued2003en_HK
dc.identifier.citationComparative Biochemistry And Physiology - B Biochemistry And Molecular Biology, 2003, v. 136 n. 2, p. 163-172en_HK
dc.identifier.issn1096-4959en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92700-
dc.description.abstractWe have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. © 2003 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cbpben_HK
dc.relation.ispartofComparative Biochemistry and Physiology - B Biochemistry and Molecular Biologyen_HK
dc.subjectCITEDen_HK
dc.subjectCo-transactivatoren_HK
dc.subjectCtenopharyngodon idellusen_HK
dc.subjectGene regulationen_HK
dc.subjectGrass carpen_HK
dc.subjectHypoxiaen_HK
dc.subjectRACE PCRen_HK
dc.titleMolecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carpen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1096-4959(03)00224-0en_HK
dc.identifier.pmid14529742-
dc.identifier.scopuseid_2-s2.0-1542398686en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542398686&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume136en_HK
dc.identifier.issue2en_HK
dc.identifier.spage163en_HK
dc.identifier.epage172en_HK
dc.identifier.isiWOS:000185879000002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, PKS=13908200700en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridZhang, ZP=35228251200en_HK
dc.identifier.scopusauthoridMok, HOL=7005842490en_HK
dc.identifier.scopusauthoridRandall, DJ=7202208365en_HK
dc.identifier.scopusauthoridKong, RYC=7005290687en_HK

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