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Article: Effects of 20 PBDE metabolites on steroidogenesis in the H295R cell line
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TitleEffects of 20 PBDE metabolites on steroidogenesis in the H295R cell line
 
AuthorsHe, Y3
Murphy, MB3
Yu, RMK3
Lam, MHW3
Hecker, M1 2
Giesy, JP3 4 1
Wu, RSS3
Lam, PKS3
 
KeywordsPBDE metabolites
Steroidogenesis
The H295R cell line
 
Issue Date2008
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxlet
 
CitationToxicology Letters, 2008, v. 176 n. 3, p. 230-238 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.toxlet.2007.12.001
 
AbstractPolybrominated diphenyl ethers (PBDEs) are additive flame retardants that have been found in the environment as well as human tissues. Environmental concentrations of these compounds have been increasing in many parts of the world in recent years. Due to their structural similarity, PBDEs are believed to have similar toxicity to PCBs, but their toxicological properties are still being determined. In this study, the steroidogenic effects of hydroxylated, methoxylated and/or chlorinated derivatives of PBDEs were assessed at both the gene and enzyme/hormone levels in the H295R human adrenocortical carcinoma cell line. The expression levels of 10 steroidogenic genes were measured using quantitative real-time PCR (Q-RT-PCR). Aromatase activity in the cells and sex steroid (testosterone (T) and 17β-estradiol (E2)) concentrations in the culture medium were also measured. CYP11B2, which regulates the synthesis of aldosterone, was the most sensitive gene and was induced by most of the compounds tested in this study. CYP19 gene expression, aromatase activity, and E2 production were also affected by several metabolites, but no consistent relationship was observed between these endpoints. Several PBDE metabolites showed some potential ability to interfere with steroidogenesis, including 5-Cl-6-OH-BDE-47, a biologically relevant BDE-47 metabolite, which significantly decreased aromatase activity and E2 production at a concentration of 10 μM. The results of this study indicate that PBDE metabolites affect steroidogenesis in vitro and that they may have the potential to affect steroidogenesis and reproduction in whole organisms. © 2007 Elsevier Ireland Ltd. All rights reserved.
 
ISSN0378-4274
2013 Impact Factor: 3.355
2013 SCImago Journal Rankings: 1.098
 
DOIhttp://dx.doi.org/10.1016/j.toxlet.2007.12.001
 
ISI Accession Number IDWOS:000254076700008
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHe, Y
 
dc.contributor.authorMurphy, MB
 
dc.contributor.authorYu, RMK
 
dc.contributor.authorLam, MHW
 
dc.contributor.authorHecker, M
 
dc.contributor.authorGiesy, JP
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorLam, PKS
 
dc.date.accessioned2010-09-17T10:54:34Z
 
dc.date.available2010-09-17T10:54:34Z
 
dc.date.issued2008
 
dc.description.abstractPolybrominated diphenyl ethers (PBDEs) are additive flame retardants that have been found in the environment as well as human tissues. Environmental concentrations of these compounds have been increasing in many parts of the world in recent years. Due to their structural similarity, PBDEs are believed to have similar toxicity to PCBs, but their toxicological properties are still being determined. In this study, the steroidogenic effects of hydroxylated, methoxylated and/or chlorinated derivatives of PBDEs were assessed at both the gene and enzyme/hormone levels in the H295R human adrenocortical carcinoma cell line. The expression levels of 10 steroidogenic genes were measured using quantitative real-time PCR (Q-RT-PCR). Aromatase activity in the cells and sex steroid (testosterone (T) and 17β-estradiol (E2)) concentrations in the culture medium were also measured. CYP11B2, which regulates the synthesis of aldosterone, was the most sensitive gene and was induced by most of the compounds tested in this study. CYP19 gene expression, aromatase activity, and E2 production were also affected by several metabolites, but no consistent relationship was observed between these endpoints. Several PBDE metabolites showed some potential ability to interfere with steroidogenesis, including 5-Cl-6-OH-BDE-47, a biologically relevant BDE-47 metabolite, which significantly decreased aromatase activity and E2 production at a concentration of 10 μM. The results of this study indicate that PBDE metabolites affect steroidogenesis in vitro and that they may have the potential to affect steroidogenesis and reproduction in whole organisms. © 2007 Elsevier Ireland Ltd. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationToxicology Letters, 2008, v. 176 n. 3, p. 230-238 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.toxlet.2007.12.001
 
dc.identifier.citeulike9570974
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.toxlet.2007.12.001
 
dc.identifier.epage238
 
dc.identifier.isiWOS:000254076700008
 
dc.identifier.issn0378-4274
2013 Impact Factor: 3.355
2013 SCImago Journal Rankings: 1.098
 
dc.identifier.issue3
 
dc.identifier.pmid18248924
 
dc.identifier.scopuseid_2-s2.0-38749113896
 
dc.identifier.spage230
 
dc.identifier.urihttp://hdl.handle.net/10722/92699
 
dc.identifier.volume176
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxlet
 
dc.publisher.placeIreland
 
dc.relation.ispartofToxicology Letters
 
dc.relation.referencesReferences in Scopus
 
dc.subjectPBDE metabolites
 
dc.subjectSteroidogenesis
 
dc.subjectThe H295R cell line
 
dc.titleEffects of 20 PBDE metabolites on steroidogenesis in the H295R cell line
 
dc.typeArticle
 
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<contributor.author>Hecker, M</contributor.author>
<contributor.author>Giesy, JP</contributor.author>
<contributor.author>Wu, RSS</contributor.author>
<contributor.author>Lam, PKS</contributor.author>
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Author Affiliations
  1. University of Saskatchewan
  2. ENTRIX Inc.
  3. City University of Hong Kong
  4. National Food Safety and Toxicology Center