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Article: Cloning and expression analysis of two distinct HIF-alpha isoforms - gcHIF-1alpha and gcHIF-4alpha - From the hypoxia-tolerant grass carp, Ctenopharyngodon idellus

TitleCloning and expression analysis of two distinct HIF-alpha isoforms - gcHIF-1alpha and gcHIF-4alpha - From the hypoxia-tolerant grass carp, Ctenopharyngodon idellus
Authors
KeywordsAmino Acid Analysis
Animal Cell
Article
Carp
Controlled Study
Fish
Gene Expression
Genetic Analysis
Genetic Difference
Genetic Similarity
Grass
Hypoxia
Molecular Cloning
Molecular Mechanics
Nonhuman
Northern Blotting
Nucleotide Sequence
Parsimony Analysis
Protein Analysis
Protein Function
Species Difference
Transcription Initiation
Transcription Regulation
Translation Regulation
Vertebrate
Western Blotting
Issue Date2006
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmolbiol/
Citation
Bmc Molecular Biology, 2006, v. 7 How to Cite?
AbstractBackground: Hypoxia-inducible factors (HIFs) are involved in adaptive and survival responses to hypoxic stress in mammals. In fish, very little is known about the functions of HIFs. Results: We have cloned and characterized two distinct HIF-alpha cDNAs - gcHIF-1alpha and gcHIF-4alpha - from the hypoxia-tolerant grass carp. The deduced gcHIF-1alpha protein is highly similar to the HIF-1alphas (57-68%) from various vertebrate species, while gcHIF-4alpha is a novel isoform, and shows an equivalent degree of amino acid identity (41-47%) to the HIF-1alpha, HIF-2alpha and HIF-3alpha proteins so far described. Parsimony analysis indicated that gcHIF-4alpha is most closely related to the HIF-3alpha proteins. Northern blot analysis showed that mRNA levels of gcHIF-1alpha and gcHIF-4alpha differ substantially under normoxic and hypoxic conditions, while Western blot studies demonstrated that the endogenous protein levels for both gcHIF-1alpha and gcHIF-4alpha are similarly responsive to hypoxia. Our findings suggest that both gcHIF-1alpha and gcHIF-4alpha are differentially regulated at the transcriptional and translational levels. HRE-luciferase reporter assays show that both proteins function as transcription activators and play distinct roles in modulating the hypoxic response in grass carp. Conclusion: There are at least two distinct HIF-alpha isoforms - gcHIF-1alpha and gcHIF-4alpha - in the hypoxia-tolerant grass carp, which are differentially expressed and regulated in different fish organs in response to hypoxic stress. Overall, the results suggest that unique molecular mechanisms operate through these two HIF-alpha isoforms, which underpin the hypoxic response in the hypoxia-tolerant grass carp. © 2006 Law et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/92697
ISSN
2015 Impact Factor: 2.5
2015 SCImago Journal Rankings: 1.224
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLaw, SHWen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorNg, PKSen_HK
dc.contributor.authorYu, RMKen_HK
dc.contributor.authorKong, RYCen_HK
dc.date.accessioned2010-09-17T10:54:30Z-
dc.date.available2010-09-17T10:54:30Z-
dc.date.issued2006en_HK
dc.identifier.citationBmc Molecular Biology, 2006, v. 7en_HK
dc.identifier.issn1471-2199en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92697-
dc.description.abstractBackground: Hypoxia-inducible factors (HIFs) are involved in adaptive and survival responses to hypoxic stress in mammals. In fish, very little is known about the functions of HIFs. Results: We have cloned and characterized two distinct HIF-alpha cDNAs - gcHIF-1alpha and gcHIF-4alpha - from the hypoxia-tolerant grass carp. The deduced gcHIF-1alpha protein is highly similar to the HIF-1alphas (57-68%) from various vertebrate species, while gcHIF-4alpha is a novel isoform, and shows an equivalent degree of amino acid identity (41-47%) to the HIF-1alpha, HIF-2alpha and HIF-3alpha proteins so far described. Parsimony analysis indicated that gcHIF-4alpha is most closely related to the HIF-3alpha proteins. Northern blot analysis showed that mRNA levels of gcHIF-1alpha and gcHIF-4alpha differ substantially under normoxic and hypoxic conditions, while Western blot studies demonstrated that the endogenous protein levels for both gcHIF-1alpha and gcHIF-4alpha are similarly responsive to hypoxia. Our findings suggest that both gcHIF-1alpha and gcHIF-4alpha are differentially regulated at the transcriptional and translational levels. HRE-luciferase reporter assays show that both proteins function as transcription activators and play distinct roles in modulating the hypoxic response in grass carp. Conclusion: There are at least two distinct HIF-alpha isoforms - gcHIF-1alpha and gcHIF-4alpha - in the hypoxia-tolerant grass carp, which are differentially expressed and regulated in different fish organs in response to hypoxic stress. Overall, the results suggest that unique molecular mechanisms operate through these two HIF-alpha isoforms, which underpin the hypoxic response in the hypoxia-tolerant grass carp. © 2006 Law et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmolbiol/en_HK
dc.relation.ispartofBMC Molecular Biologyen_HK
dc.subjectAmino Acid Analysisen_HK
dc.subjectAnimal Cellen_HK
dc.subjectArticleen_HK
dc.subjectCarpen_HK
dc.subjectControlled Studyen_HK
dc.subjectFishen_HK
dc.subjectGene Expressionen_HK
dc.subjectGenetic Analysisen_HK
dc.subjectGenetic Differenceen_HK
dc.subjectGenetic Similarityen_HK
dc.subjectGrassen_HK
dc.subjectHypoxiaen_HK
dc.subjectMolecular Cloningen_HK
dc.subjectMolecular Mechanicsen_HK
dc.subjectNonhumanen_HK
dc.subjectNorthern Blottingen_HK
dc.subjectNucleotide Sequenceen_HK
dc.subjectParsimony Analysisen_HK
dc.subjectProtein Analysisen_HK
dc.subjectProtein Functionen_HK
dc.subjectSpecies Differenceen_HK
dc.subjectTranscription Initiationen_HK
dc.subjectTranscription Regulationen_HK
dc.subjectTranslation Regulationen_HK
dc.subjectVertebrateen_HK
dc.subjectWestern Blottingen_HK
dc.titleCloning and expression analysis of two distinct HIF-alpha isoforms - gcHIF-1alpha and gcHIF-4alpha - From the hypoxia-tolerant grass carp, Ctenopharyngodon idellusen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1471-2199-7-15en_HK
dc.identifier.pmid16623959-
dc.identifier.pmcidPMC1473195-
dc.identifier.scopuseid_2-s2.0-33744797630en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33744797630&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.isiWOS:000238398400001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLaw, SHW=7202242024en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridNg, PKS=13908200700en_HK
dc.identifier.scopusauthoridYu, RMK=9278574900en_HK
dc.identifier.scopusauthoridKong, RYC=7005290687en_HK
dc.identifier.citeulike687145-

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