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Article: Assessment of the effects of chemicals on the expression of ten steroidogenic genes in the H295R cell line using real-time PCR

TitleAssessment of the effects of chemicals on the expression of ten steroidogenic genes in the H295R cell line using real-time PCR
Authors
KeywordsBioassay
Screening
Steroidogenesis
Xenoestrogens
Issue Date2004
PublisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/
Citation
Toxicological Sciences, 2004, v. 81 n. 1, p. 78-89 How to Cite?
AbstractThe potential for a variety of environmental contaminants to disturb endocrine function in wildlife and humans has been of recent concern. While much effort is being focused on the assessment of effects mediated through steroid hormone receptor-based mechanisms, there are potentially several other mechanisms that could lead to endocrine disruption. Recent studies have demonstrated that a variety of xenobiotics can alter the gene expression or activity of enzymes involved in steroidogenesis. By altering the production or catalytic activity of steroidogenic or steroid-catabolizing enzymes, these chemicals have the potential to alter the steroid balance in organisms. To assess the potential of chemicals to alter steroidogenesis, an assay system was developed using a human adrenocortical carcinoma cell line, the H295R cell line, which retains the ability to synthesize most of the important steroidogenic enzymes. Methods were developed, optimized, and validated to measure the expression of 10 genes involved in steroidogenesis by the use of real-time quantitative reverse transcriptase PCR. The effects of several model chemicals known to alter steroid metabolism, both inducers and inhibitors, were assessed. Similar expression patterns were observed for chemicals acting through common mechanisms of action. Time-course studies demonstrated distinct time-dependent expression profiles for chemicals able to modulate steroid metabolism. The assay, which allows simultaneous analysis of the expression of numerous steroidogenic enzymes, would be useful as a sensitive and integrative screen for the many effects of chemicals on steroidogenesis. © Society of Toxicology 2004; all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92696
ISSN
2014 Impact Factor: 3.854
2014 SCImago Journal Rankings: 1.542
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHilscherova, Ken_HK
dc.contributor.authorJones, PDen_HK
dc.contributor.authorGracia, Ten_HK
dc.contributor.authorNewsted, JLen_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorSanderson, JTen_HK
dc.contributor.authorYu, RMKen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorGiesy, JPen_HK
dc.date.accessioned2010-09-17T10:54:28Z-
dc.date.available2010-09-17T10:54:28Z-
dc.date.issued2004en_HK
dc.identifier.citationToxicological Sciences, 2004, v. 81 n. 1, p. 78-89en_HK
dc.identifier.issn1096-6080en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92696-
dc.description.abstractThe potential for a variety of environmental contaminants to disturb endocrine function in wildlife and humans has been of recent concern. While much effort is being focused on the assessment of effects mediated through steroid hormone receptor-based mechanisms, there are potentially several other mechanisms that could lead to endocrine disruption. Recent studies have demonstrated that a variety of xenobiotics can alter the gene expression or activity of enzymes involved in steroidogenesis. By altering the production or catalytic activity of steroidogenic or steroid-catabolizing enzymes, these chemicals have the potential to alter the steroid balance in organisms. To assess the potential of chemicals to alter steroidogenesis, an assay system was developed using a human adrenocortical carcinoma cell line, the H295R cell line, which retains the ability to synthesize most of the important steroidogenic enzymes. Methods were developed, optimized, and validated to measure the expression of 10 genes involved in steroidogenesis by the use of real-time quantitative reverse transcriptase PCR. The effects of several model chemicals known to alter steroid metabolism, both inducers and inhibitors, were assessed. Similar expression patterns were observed for chemicals acting through common mechanisms of action. Time-course studies demonstrated distinct time-dependent expression profiles for chemicals able to modulate steroid metabolism. The assay, which allows simultaneous analysis of the expression of numerous steroidogenic enzymes, would be useful as a sensitive and integrative screen for the many effects of chemicals on steroidogenesis. © Society of Toxicology 2004; all rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/en_HK
dc.relation.ispartofToxicological Sciencesen_HK
dc.subjectBioassayen_HK
dc.subjectScreeningen_HK
dc.subjectSteroidogenesisen_HK
dc.subjectXenoestrogensen_HK
dc.titleAssessment of the effects of chemicals on the expression of ten steroidogenic genes in the H295R cell line using real-time PCRen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/toxsci/kfh191en_HK
dc.identifier.pmid15187238-
dc.identifier.scopuseid_2-s2.0-4444240722en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4444240722&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume81en_HK
dc.identifier.issue1en_HK
dc.identifier.spage78en_HK
dc.identifier.epage89en_HK
dc.identifier.isiWOS:000223589100011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHilscherova, K=6603438103en_HK
dc.identifier.scopusauthoridJones, PD=34771015600en_HK
dc.identifier.scopusauthoridGracia, T=6506058618en_HK
dc.identifier.scopusauthoridNewsted, JL=6603677236en_HK
dc.identifier.scopusauthoridZhang, X=8606600100en_HK
dc.identifier.scopusauthoridSanderson, JT=7004489858en_HK
dc.identifier.scopusauthoridYu, RMK=9278574900en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridGiesy, JP=35459135300en_HK

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