Article: Assessment of the effects of chemicals on the expression of ten steroidogenic genes in the H295R cell line using real-time PCR

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TitleAssessment of the effects of chemicals on the expression of ten steroidogenic genes in the H295R cell line using real-time PCR
AuthorsHilscherova, K1
Jones, PD1
Gracia, T1
Newsted, JL2
Zhang, X1 3
Sanderson, JT4
Yu, RMK3
Wu, RSS3
Giesy, JP1 3
KeywordsBioassay
Screening
Steroidogenesis
Xenoestrogens
Issue Date2004
PublisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/
CitationToxicological Sciences, 2004, v. 81 n. 1, p. 78-89 [How to Cite?]
DOI: http://dx.doi.org/10.1093/toxsci/kfh191
AbstractThe potential for a variety of environmental contaminants to disturb endocrine function in wildlife and humans has been of recent concern. While much effort is being focused on the assessment of effects mediated through steroid hormone receptor-based mechanisms, there are potentially several other mechanisms that could lead to endocrine disruption. Recent studies have demonstrated that a variety of xenobiotics can alter the gene expression or activity of enzymes involved in steroidogenesis. By altering the production or catalytic activity of steroidogenic or steroid-catabolizing enzymes, these chemicals have the potential to alter the steroid balance in organisms. To assess the potential of chemicals to alter steroidogenesis, an assay system was developed using a human adrenocortical carcinoma cell line, the H295R cell line, which retains the ability to synthesize most of the important steroidogenic enzymes. Methods were developed, optimized, and validated to measure the expression of 10 genes involved in steroidogenesis by the use of real-time quantitative reverse transcriptase PCR. The effects of several model chemicals known to alter steroid metabolism, both inducers and inhibitors, were assessed. Similar expression patterns were observed for chemicals acting through common mechanisms of action. Time-course studies demonstrated distinct time-dependent expression profiles for chemicals able to modulate steroid metabolism. The assay, which allows simultaneous analysis of the expression of numerous steroidogenic enzymes, would be useful as a sensitive and integrative screen for the many effects of chemicals on steroidogenesis. © Society of Toxicology 2004; all rights reserved.
ISSN1096-6080
2011 Impact Factor: 4.652
2011 SCImago Journal Rankings: 0.307
DOIhttp://dx.doi.org/10.1093/toxsci/kfh191
ISI Accession Number IDWOS:000223589100011
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorHilscherova, K
dc.contributor.authorJones, PD
dc.contributor.authorGracia, T
dc.contributor.authorNewsted, JL
dc.contributor.authorZhang, X
dc.contributor.authorSanderson, JT
dc.contributor.authorYu, RMK
dc.contributor.authorWu, RSS
dc.contributor.authorGiesy, JP
dc.date.accessioned2010-09-17T10:54:28Z
dc.date.available2010-09-17T10:54:28Z
dc.date.issued2004
dc.description.abstractThe potential for a variety of environmental contaminants to disturb endocrine function in wildlife and humans has been of recent concern. While much effort is being focused on the assessment of effects mediated through steroid hormone receptor-based mechanisms, there are potentially several other mechanisms that could lead to endocrine disruption. Recent studies have demonstrated that a variety of xenobiotics can alter the gene expression or activity of enzymes involved in steroidogenesis. By altering the production or catalytic activity of steroidogenic or steroid-catabolizing enzymes, these chemicals have the potential to alter the steroid balance in organisms. To assess the potential of chemicals to alter steroidogenesis, an assay system was developed using a human adrenocortical carcinoma cell line, the H295R cell line, which retains the ability to synthesize most of the important steroidogenic enzymes. Methods were developed, optimized, and validated to measure the expression of 10 genes involved in steroidogenesis by the use of real-time quantitative reverse transcriptase PCR. The effects of several model chemicals known to alter steroid metabolism, both inducers and inhibitors, were assessed. Similar expression patterns were observed for chemicals acting through common mechanisms of action. Time-course studies demonstrated distinct time-dependent expression profiles for chemicals able to modulate steroid metabolism. The assay, which allows simultaneous analysis of the expression of numerous steroidogenic enzymes, would be useful as a sensitive and integrative screen for the many effects of chemicals on steroidogenesis. © Society of Toxicology 2004; all rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationToxicological Sciences, 2004, v. 81 n. 1, p. 78-89 [How to Cite?]
DOI: http://dx.doi.org/10.1093/toxsci/kfh191
dc.identifier.doihttp://dx.doi.org/10.1093/toxsci/kfh191
dc.identifier.epage89
dc.identifier.isiWOS:000223589100011
dc.identifier.issn1096-6080
2011 Impact Factor: 4.652
2011 SCImago Journal Rankings: 0.307
dc.identifier.issue1
dc.identifier.pmid15187238
dc.identifier.scopuseid_2-s2.0-4444240722
dc.identifier.spage78
dc.identifier.urihttp://hdl.handle.net/10722/92696
dc.identifier.volume81
dc.languageeng
dc.publisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofToxicological Sciences
dc.relation.referencesReferences in Scopus
dc.subjectBioassay
dc.subjectScreening
dc.subjectSteroidogenesis
dc.subjectXenoestrogens
dc.titleAssessment of the effects of chemicals on the expression of ten steroidogenic genes in the H295R cell line using real-time PCR
dc.typeArticle
Author Affiliations
  1. Michigan State University
  2. ENTRIX Inc.
  3. City University of Hong Kong
  4. Utrecht University