File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Effects of PCBs and MeSO2-PCBs on adrenocortical steroidogenesis in H295R human adrenocortical carcinoma cells
  • Basic View
  • Metadata View
  • XML View
TitleEffects of PCBs and MeSO2-PCBs on adrenocortical steroidogenesis in H295R human adrenocortical carcinoma cells
 
AuthorsXu, Y2
Yu, RMK2
Zhang, X2 3
Murphy, MB2 3
Giesy, JP2 3
Lam, MHW2
Lam, PKS2
Wu, RSS2
Yu, H1
 
KeywordsH295R
MeSO2-PCBs
Polychlorinated biphenyls
Steroidogenesis
 
Issue Date2006
 
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
 
CitationChemosphere, 2006, v. 63 n. 5, p. 772-784 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.chemosphere.2005.08.013
 
AbstractSome endocrine disrupting chemicals (EDCs) in the environment have been shown to exert their biological effects through interference with steroidogenesis. In this study, the potential effects of four selected polychlorinated biphenyl (PCB) congeners (PCB101, PCB110, PCB126 and PCB149) as well as several of their environmentally-relevant methylsulfonyl-(MeSO2-) PCB metabolites (3′-MeSO2-CB101, 4′-MeSO2-CB101, 4′-MeSO2-CB110, 3′-MeSO2-CB149 and 4′-MeSO2-CB149) on adrenocortical steroidogenesis were evaluated by in vitro bioassay based on the human adrenocortical carcinoma H295R cell line. The PCBs included in the study represented different structures and potential mechanisms of action. Cells were exposed for 48 h to 10 μM of each PCB congener in the presence or absence of 20% (w/w) of their corresponding MeSO2-PCB metabolite(s). After the chemical treatments, changes in mRNA expression of 11 steroidogenic genes (CYP11A, CYP11B1, CYP11B2, CYP17, CYP19, CYP21, 3β-HSD1, 3β-HSD2, 17β-HSD1, StAR and HMGR) were quantified using molecular beacon-based real-time RT-PCR. Genes coding for enzymes involved in the later or final steps of steroid production (CYP11B1, CYP11B2, CYP19, 3β-HSD1, 3β-HSD2 and 17β-HSD1) were up-regulated to various extents by most PCBs. The greatest transcriptional activations (2.8-29.9-fold) were elicited by PCB110 on CYP11B1, CYP11B2, 3β-HSD2 and CYP19, and PCB149 on CYP11B1, 3β-HSD1 and 17β-HSD1. Increased expression of these steroidogenic genes might ultimately lead to a change in hormonal balance through excessive production of steroid hormones including aldosterone, cortisol and estradiol. In addition, co-treatment with 3′- and 4′-MeSO2-PCB149 resulted in a significant decrease in PCB149-induced 3β-HSD1 and 17β-HSD1 expression. This result indicates that some PCB congeners and their MeSO2-metabolites may affect steroidogenesis via different mechanisms. Overall, these findings suggest that PCBs and PCB metabolites can affect regulation of adrenocortical steroidogenesis. © 2005 Elsevier Ltd. All rights reserved.
 
ISSN0045-6535
2013 Impact Factor: 3.499
2013 SCImago Journal Rankings: 1.746
 
DOIhttp://dx.doi.org/10.1016/j.chemosphere.2005.08.013
 
ISI Accession Number IDWOS:000237648900008
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorXu, Y
 
dc.contributor.authorYu, RMK
 
dc.contributor.authorZhang, X
 
dc.contributor.authorMurphy, MB
 
dc.contributor.authorGiesy, JP
 
dc.contributor.authorLam, MHW
 
dc.contributor.authorLam, PKS
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorYu, H
 
dc.date.accessioned2010-09-17T10:54:19Z
 
dc.date.available2010-09-17T10:54:19Z
 
dc.date.issued2006
 
dc.description.abstractSome endocrine disrupting chemicals (EDCs) in the environment have been shown to exert their biological effects through interference with steroidogenesis. In this study, the potential effects of four selected polychlorinated biphenyl (PCB) congeners (PCB101, PCB110, PCB126 and PCB149) as well as several of their environmentally-relevant methylsulfonyl-(MeSO2-) PCB metabolites (3′-MeSO2-CB101, 4′-MeSO2-CB101, 4′-MeSO2-CB110, 3′-MeSO2-CB149 and 4′-MeSO2-CB149) on adrenocortical steroidogenesis were evaluated by in vitro bioassay based on the human adrenocortical carcinoma H295R cell line. The PCBs included in the study represented different structures and potential mechanisms of action. Cells were exposed for 48 h to 10 μM of each PCB congener in the presence or absence of 20% (w/w) of their corresponding MeSO2-PCB metabolite(s). After the chemical treatments, changes in mRNA expression of 11 steroidogenic genes (CYP11A, CYP11B1, CYP11B2, CYP17, CYP19, CYP21, 3β-HSD1, 3β-HSD2, 17β-HSD1, StAR and HMGR) were quantified using molecular beacon-based real-time RT-PCR. Genes coding for enzymes involved in the later or final steps of steroid production (CYP11B1, CYP11B2, CYP19, 3β-HSD1, 3β-HSD2 and 17β-HSD1) were up-regulated to various extents by most PCBs. The greatest transcriptional activations (2.8-29.9-fold) were elicited by PCB110 on CYP11B1, CYP11B2, 3β-HSD2 and CYP19, and PCB149 on CYP11B1, 3β-HSD1 and 17β-HSD1. Increased expression of these steroidogenic genes might ultimately lead to a change in hormonal balance through excessive production of steroid hormones including aldosterone, cortisol and estradiol. In addition, co-treatment with 3′- and 4′-MeSO2-PCB149 resulted in a significant decrease in PCB149-induced 3β-HSD1 and 17β-HSD1 expression. This result indicates that some PCB congeners and their MeSO2-metabolites may affect steroidogenesis via different mechanisms. Overall, these findings suggest that PCBs and PCB metabolites can affect regulation of adrenocortical steroidogenesis. © 2005 Elsevier Ltd. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationChemosphere, 2006, v. 63 n. 5, p. 772-784 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.chemosphere.2005.08.013
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.chemosphere.2005.08.013
 
dc.identifier.epage784
 
dc.identifier.isiWOS:000237648900008
 
dc.identifier.issn0045-6535
2013 Impact Factor: 3.499
2013 SCImago Journal Rankings: 1.746
 
dc.identifier.issue5
 
dc.identifier.pmid16216300
 
dc.identifier.scopuseid_2-s2.0-33646066554
 
dc.identifier.spage772
 
dc.identifier.urihttp://hdl.handle.net/10722/92691
 
dc.identifier.volume63
 
dc.languageeng
 
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofChemosphere
 
dc.relation.referencesReferences in Scopus
 
dc.subjectH295R
 
dc.subjectMeSO2-PCBs
 
dc.subjectPolychlorinated biphenyls
 
dc.subjectSteroidogenesis
 
dc.titleEffects of PCBs and MeSO2-PCBs on adrenocortical steroidogenesis in H295R human adrenocortical carcinoma cells
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Xu, Y</contributor.author>
<contributor.author>Yu, RMK</contributor.author>
<contributor.author>Zhang, X</contributor.author>
<contributor.author>Murphy, MB</contributor.author>
<contributor.author>Giesy, JP</contributor.author>
<contributor.author>Lam, MHW</contributor.author>
<contributor.author>Lam, PKS</contributor.author>
<contributor.author>Wu, RSS</contributor.author>
<contributor.author>Yu, H</contributor.author>
<date.accessioned>2010-09-17T10:54:19Z</date.accessioned>
<date.available>2010-09-17T10:54:19Z</date.available>
<date.issued>2006</date.issued>
<identifier.citation>Chemosphere, 2006, v. 63 n. 5, p. 772-784</identifier.citation>
<identifier.issn>0045-6535</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/92691</identifier.uri>
<description.abstract>Some endocrine disrupting chemicals (EDCs) in the environment have been shown to exert their biological effects through interference with steroidogenesis. In this study, the potential effects of four selected polychlorinated biphenyl (PCB) congeners (PCB101, PCB110, PCB126 and PCB149) as well as several of their environmentally-relevant methylsulfonyl-(MeSO2-) PCB metabolites (3&#8242;-MeSO2-CB101, 4&#8242;-MeSO2-CB101, 4&#8242;-MeSO2-CB110, 3&#8242;-MeSO2-CB149 and 4&#8242;-MeSO2-CB149) on adrenocortical steroidogenesis were evaluated by in vitro bioassay based on the human adrenocortical carcinoma H295R cell line. The PCBs included in the study represented different structures and potential mechanisms of action. Cells were exposed for 48 h to 10 &#956;M of each PCB congener in the presence or absence of 20% (w/w) of their corresponding MeSO2-PCB metabolite(s). After the chemical treatments, changes in mRNA expression of 11 steroidogenic genes (CYP11A, CYP11B1, CYP11B2, CYP17, CYP19, CYP21, 3&#946;-HSD1, 3&#946;-HSD2, 17&#946;-HSD1, StAR and HMGR) were quantified using molecular beacon-based real-time RT-PCR. Genes coding for enzymes involved in the later or final steps of steroid production (CYP11B1, CYP11B2, CYP19, 3&#946;-HSD1, 3&#946;-HSD2 and 17&#946;-HSD1) were up-regulated to various extents by most PCBs. The greatest transcriptional activations (2.8-29.9-fold) were elicited by PCB110 on CYP11B1, CYP11B2, 3&#946;-HSD2 and CYP19, and PCB149 on CYP11B1, 3&#946;-HSD1 and 17&#946;-HSD1. Increased expression of these steroidogenic genes might ultimately lead to a change in hormonal balance through excessive production of steroid hormones including aldosterone, cortisol and estradiol. In addition, co-treatment with 3&#8242;- and 4&#8242;-MeSO2-PCB149 resulted in a significant decrease in PCB149-induced 3&#946;-HSD1 and 17&#946;-HSD1 expression. This result indicates that some PCB congeners and their MeSO2-metabolites may affect steroidogenesis via different mechanisms. Overall, these findings suggest that PCBs and PCB metabolites can affect regulation of adrenocortical steroidogenesis. &#169; 2005 Elsevier Ltd. All rights reserved.</description.abstract>
<language>eng</language>
<publisher>Pergamon. The Journal&apos;s web site is located at http://www.elsevier.com/locate/chemosphere</publisher>
<relation.ispartof>Chemosphere</relation.ispartof>
<subject>H295R</subject>
<subject>MeSO2-PCBs</subject>
<subject>Polychlorinated biphenyls</subject>
<subject>Steroidogenesis</subject>
<title>Effects of PCBs and MeSO2-PCBs on adrenocortical steroidogenesis in H295R human adrenocortical carcinoma cells</title>
<type>Article</type>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1016/j.chemosphere.2005.08.013</identifier.doi>
<identifier.pmid>16216300</identifier.pmid>
<identifier.scopus>eid_2-s2.0-33646066554</identifier.scopus>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-33646066554&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>63</identifier.volume>
<identifier.issue>5</identifier.issue>
<identifier.spage>772</identifier.spage>
<identifier.epage>784</identifier.epage>
<identifier.isi>WOS:000237648900008</identifier.isi>
<publisher.place>United Kingdom</publisher.place>
</item>
Author Affiliations
  1. Nanjing University
  2. City University of Hong Kong
  3. National Food Safety and Toxicology Center