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Article: Whole-mount in situ TUNEL method revealed ectopic pattern of apoptosis in cadmium treated naupliar larvae of barnacle (Balanus amphitrite Darwin)

TitleWhole-mount in situ TUNEL method revealed ectopic pattern of apoptosis in cadmium treated naupliar larvae of barnacle (Balanus amphitrite Darwin)
Authors
KeywordsApoptosis
Barnacle larvae
Cadmium
Swimming behaviour
TUNEL
Issue Date2004
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
Citation
Chemosphere, 2004, v. 55 n. 10, p. 1387-1394 How to Cite?
AbstractThe effect of cadmium on stage II naupliar larvae of barnacle (Balanus amphitrite Darwin) was investigated. Barnacle larvae were exposed to 0-15 μM CdCl2 for 24 h. Apoptotic cells were stained by the terminal deoxyribonucleotidyl transferase mediated dUTP nick end labelling (TUNEL) method. Incidence of apoptosis, as measured by numbers of animals with ectopic pattern of apoptosis as well as numbers of apoptotic cells per animal, was assessed using confocal microscopy. An increase in incidence of apoptosis was observed in the experimental animals with an increase in cadmium concentration. Mortality increased, and motility decreased, when barnacle larvae were exposed to an increasing concentration of cadmium. The relationship between the occurrence of apoptosis and swimming behaviour was investigated in larvae exposed to 10 μM CdCl2. Significant increases in apoptosis were detected in the non-motile and dead nauplii. This study suggested that whole-mount in situ TUNEL method may be used to study increased occurrence of apoptotic cells in crustacean larvae. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92684
ISSN
2015 Impact Factor: 3.698
2015 SCImago Journal Rankings: 1.536
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheng, SHen_HK
dc.contributor.authorChan, KWen_HK
dc.contributor.authorChan, PKen_HK
dc.contributor.authorSo, CHen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWu, RSSen_HK
dc.date.accessioned2010-09-17T10:54:06Z-
dc.date.available2010-09-17T10:54:06Z-
dc.date.issued2004en_HK
dc.identifier.citationChemosphere, 2004, v. 55 n. 10, p. 1387-1394en_HK
dc.identifier.issn0045-6535en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92684-
dc.description.abstractThe effect of cadmium on stage II naupliar larvae of barnacle (Balanus amphitrite Darwin) was investigated. Barnacle larvae were exposed to 0-15 μM CdCl2 for 24 h. Apoptotic cells were stained by the terminal deoxyribonucleotidyl transferase mediated dUTP nick end labelling (TUNEL) method. Incidence of apoptosis, as measured by numbers of animals with ectopic pattern of apoptosis as well as numbers of apoptotic cells per animal, was assessed using confocal microscopy. An increase in incidence of apoptosis was observed in the experimental animals with an increase in cadmium concentration. Mortality increased, and motility decreased, when barnacle larvae were exposed to an increasing concentration of cadmium. The relationship between the occurrence of apoptosis and swimming behaviour was investigated in larvae exposed to 10 μM CdCl2. Significant increases in apoptosis were detected in the non-motile and dead nauplii. This study suggested that whole-mount in situ TUNEL method may be used to study increased occurrence of apoptotic cells in crustacean larvae. © 2004 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphereen_HK
dc.relation.ispartofChemosphereen_HK
dc.subjectApoptosisen_HK
dc.subjectBarnacle larvaeen_HK
dc.subjectCadmiumen_HK
dc.subjectSwimming behaviouren_HK
dc.subjectTUNELen_HK
dc.titleWhole-mount in situ TUNEL method revealed ectopic pattern of apoptosis in cadmium treated naupliar larvae of barnacle (Balanus amphitrite Darwin)en_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chemosphere.2004.01.011en_HK
dc.identifier.pmid15081781-
dc.identifier.scopuseid_2-s2.0-1842636925en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1842636925&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume55en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1387en_HK
dc.identifier.epage1394en_HK
dc.identifier.isiWOS:000221501700011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridCheng, SH=7404684691en_HK
dc.identifier.scopusauthoridChan, KW=29267471000en_HK
dc.identifier.scopusauthoridChan, PK=7403498052en_HK
dc.identifier.scopusauthoridSo, CH=36979763900en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK

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