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Article: Effect of hypoxia on RAW264.7 macrophages apoptosis and signaling

TitleEffect of hypoxia on RAW264.7 macrophages apoptosis and signaling
Authors
KeywordsAKT
Apoptosis
Gene expression
Hypoxia
JNK
NF-κB
RAW264.7 macrophages
Issue Date2007
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicol
Citation
Toxicology, 2007, v. 235 n. 1-2, p. 52-61 How to Cite?
AbstractHypoxia, a decrease in oxygen tension occurring in pathological tissues, has a profound effect on macrophage functions. In this study, we have investigated cellular and molecular responses of murine macrophage-like RAW264.7 cells to low oxygen tension. Our study revealed that hypoxia induced RAW264.7 cells apoptosis and cell cycle arrest at G0/G1 phase. The result of Western blotting showed that the expression of apoptosis related signaling molecules, such as AKT and JNK was activated under hypoxia. The result of electrophoretic mobility shift assay showed that the DNA binding activity of nuclear factor kappa B (NF-κB) was also increased by hypoxic stimulation. Furthermore, gene expression profiles of RAW264.7 macrophages induced by hypoxia showed that hypoxia treatment may alter expression of genes related to apoptosis, survival, cell cycle, metabolism and structural matrix. © 2007 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92680
ISSN
2015 Impact Factor: 3.817
2015 SCImago Journal Rankings: 1.335
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFong, CCen_HK
dc.contributor.authorZhang, Qen_HK
dc.contributor.authorShi, YFen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorFong, WFen_HK
dc.contributor.authorYang, Men_HK
dc.date.accessioned2010-09-17T10:53:59Z-
dc.date.available2010-09-17T10:53:59Z-
dc.date.issued2007en_HK
dc.identifier.citationToxicology, 2007, v. 235 n. 1-2, p. 52-61en_HK
dc.identifier.issn0300-483Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92680-
dc.description.abstractHypoxia, a decrease in oxygen tension occurring in pathological tissues, has a profound effect on macrophage functions. In this study, we have investigated cellular and molecular responses of murine macrophage-like RAW264.7 cells to low oxygen tension. Our study revealed that hypoxia induced RAW264.7 cells apoptosis and cell cycle arrest at G0/G1 phase. The result of Western blotting showed that the expression of apoptosis related signaling molecules, such as AKT and JNK was activated under hypoxia. The result of electrophoretic mobility shift assay showed that the DNA binding activity of nuclear factor kappa B (NF-κB) was also increased by hypoxic stimulation. Furthermore, gene expression profiles of RAW264.7 macrophages induced by hypoxia showed that hypoxia treatment may alter expression of genes related to apoptosis, survival, cell cycle, metabolism and structural matrix. © 2007 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicolen_HK
dc.relation.ispartofToxicologyen_HK
dc.subjectAKTen_HK
dc.subjectApoptosisen_HK
dc.subjectGene expressionen_HK
dc.subjectHypoxiaen_HK
dc.subjectJNKen_HK
dc.subjectNF-κBen_HK
dc.subjectRAW264.7 macrophagesen_HK
dc.titleEffect of hypoxia on RAW264.7 macrophages apoptosis and signalingen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.tox.2007.03.006en_HK
dc.identifier.pmid17416447-
dc.identifier.scopuseid_2-s2.0-34247630026en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247630026&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume235en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage52en_HK
dc.identifier.epage61en_HK
dc.identifier.isiWOS:000247046600005-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridFong, CC=11739503800en_HK
dc.identifier.scopusauthoridZhang, Q=35515964300en_HK
dc.identifier.scopusauthoridShi, YF=7404963405en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridFong, WF=7102816013en_HK
dc.identifier.scopusauthoridYang, M=35204210300en_HK

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