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Article: Cultured gill epithelial cells from tilapia (Oreochromis niloticus): A new in vitro assay for toxicants

TitleCultured gill epithelial cells from tilapia (Oreochromis niloticus): A new in vitro assay for toxicants
Authors
KeywordsB[a]P
EROD
Gill epithelia
PCB
Primary cell culture
TCDD
Tilapia
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2005, v. 71 n. 1, p. 61-72 How to Cite?
AbstractA culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained, and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3′,4,4′,5- pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC 50 of EROD activity was 2.77 × 10 -7 M for PCB#126, 1.85 × 10 -7 M for B[a]P and 7.38 × 10 -10 M for TCDD, showing that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple, rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. © 2004 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92672
ISSN
2015 Impact Factor: 3.557
2015 SCImago Journal Rankings: 1.671
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Ben_HK
dc.contributor.authorLiu, Wen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorLam, PKSen_HK
dc.date.accessioned2010-09-17T10:53:44Z-
dc.date.available2010-09-17T10:53:44Z-
dc.date.issued2005en_HK
dc.identifier.citationAquatic Toxicology, 2005, v. 71 n. 1, p. 61-72en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92672-
dc.description.abstractA culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained, and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3′,4,4′,5- pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC 50 of EROD activity was 2.77 × 10 -7 M for PCB#126, 1.85 × 10 -7 M for B[a]P and 7.38 × 10 -10 M for TCDD, showing that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple, rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. © 2004 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectB[a]Pen_HK
dc.subjectERODen_HK
dc.subjectGill epitheliaen_HK
dc.subjectPCBen_HK
dc.subjectPrimary cell cultureen_HK
dc.subjectTCDDen_HK
dc.subjectTilapiaen_HK
dc.titleCultured gill epithelial cells from tilapia (Oreochromis niloticus): A new in vitro assay for toxicantsen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2004.10.013en_HK
dc.identifier.pmid15642632-
dc.identifier.scopuseid_2-s2.0-11844273193en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-11844273193&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume71en_HK
dc.identifier.issue1en_HK
dc.identifier.spage61en_HK
dc.identifier.epage72en_HK
dc.identifier.isiWOS:000226566400006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhou, B=7401906781en_HK
dc.identifier.scopusauthoridLiu, W=26664155200en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK

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