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Article: Agreement between breast milk dioxin levels by CALUX bioassay and chemical analysis in a population survey in Hong Kong

TitleAgreement between breast milk dioxin levels by CALUX bioassay and chemical analysis in a population survey in Hong Kong
Authors
KeywordsBioassay
Breast milk
Chemical determination
Dioxins
Hong Kong
Toxic equivalent factor
Issue Date2007
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
Citation
Chemosphere, 2007, v. 69 n. 8, p. 1287-1294 How to Cite?
AbstractChemically-activated luciferase gene expression (CALUX) bioassay and gas chromatography/mass spectrometry (GC/MS) are used to determine dioxin levels in food and humans. Valid measures of the agreement between the two methods would improve interpretation of bioassay results. Paired breast milk samples from 250 mothers, as 11 pooled samples, were analysed by GC/MS for total WHO-TEQ (7 polychlorinated dibenzo-para-dioxins, 10 polychlorinated dibenzofurans and 12 dioxin-like polychlorinated biphenyls) and as individual samples by CALUX. Mean difference between total WHO-TEQ (weighted by TEF system derived in 1997) and mean CALUX-TEQ in each pool was 1.6 pg/g fat (95% CI: 0.7, 2.4), indicating a statistically significant overestimation of CALUX-TEQ compared to WHO-TEQ, probably due to the presence of Ah-receptor agonists. CALUX estimated toxicity of 13 pg/g fat was greater than the WHO-TEQ by 0.9, 3.1 and 0.3 pg/g fat for mothers from Hong Kong, mainland China and overseas territories, respectively. When the 2005 TEF system was applied, a reduction of 14-26% in the WHO-TEQ and a larger but less disperse discrepancy between WHO-TEQ and CALUX-TEQ (3.9 pg/g fat, 95% CI: 3.5, 4.4) were observed. Our study suggested that the mothers' place of residence explained the discrepancy between CALUX-TEQ and WHO-TEQ and should be considered in inter-country comparisons for CALUX-TEQ. For regulatory purposes bioassays for detecting quantitative dioxin contents in any setting must be combined with adequate extraction, clean-up and validation with WHO-TEQs. The larger difference between the two measurements after using the new TEF system warrants further investigation. © 2007 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92612
ISSN
2015 Impact Factor: 3.698
2015 SCImago Journal Rankings: 1.536
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHui, LLen_HK
dc.contributor.authorHedley, AJen_HK
dc.contributor.authorNelson, EASen_HK
dc.contributor.authorMalisch, Ren_HK
dc.contributor.authorWong, TWen_HK
dc.contributor.authorCowling, BJen_HK
dc.date.accessioned2010-09-17T10:51:41Z-
dc.date.available2010-09-17T10:51:41Z-
dc.date.issued2007en_HK
dc.identifier.citationChemosphere, 2007, v. 69 n. 8, p. 1287-1294en_HK
dc.identifier.issn0045-6535en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92612-
dc.description.abstractChemically-activated luciferase gene expression (CALUX) bioassay and gas chromatography/mass spectrometry (GC/MS) are used to determine dioxin levels in food and humans. Valid measures of the agreement between the two methods would improve interpretation of bioassay results. Paired breast milk samples from 250 mothers, as 11 pooled samples, were analysed by GC/MS for total WHO-TEQ (7 polychlorinated dibenzo-para-dioxins, 10 polychlorinated dibenzofurans and 12 dioxin-like polychlorinated biphenyls) and as individual samples by CALUX. Mean difference between total WHO-TEQ (weighted by TEF system derived in 1997) and mean CALUX-TEQ in each pool was 1.6 pg/g fat (95% CI: 0.7, 2.4), indicating a statistically significant overestimation of CALUX-TEQ compared to WHO-TEQ, probably due to the presence of Ah-receptor agonists. CALUX estimated toxicity of 13 pg/g fat was greater than the WHO-TEQ by 0.9, 3.1 and 0.3 pg/g fat for mothers from Hong Kong, mainland China and overseas territories, respectively. When the 2005 TEF system was applied, a reduction of 14-26% in the WHO-TEQ and a larger but less disperse discrepancy between WHO-TEQ and CALUX-TEQ (3.9 pg/g fat, 95% CI: 3.5, 4.4) were observed. Our study suggested that the mothers' place of residence explained the discrepancy between CALUX-TEQ and WHO-TEQ and should be considered in inter-country comparisons for CALUX-TEQ. For regulatory purposes bioassays for detecting quantitative dioxin contents in any setting must be combined with adequate extraction, clean-up and validation with WHO-TEQs. The larger difference between the two measurements after using the new TEF system warrants further investigation. © 2007 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphereen_HK
dc.relation.ispartofChemosphereen_HK
dc.subjectBioassayen_HK
dc.subjectBreast milken_HK
dc.subjectChemical determinationen_HK
dc.subjectDioxinsen_HK
dc.subjectHong Kongen_HK
dc.subjectToxic equivalent factoren_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshDioxins - analysisen_HK
dc.subject.meshEnvironmental Pollutants - analysisen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGas Chromatography-Mass Spectrometryen_HK
dc.subject.meshHong Kongen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLuciferases - analysisen_HK
dc.subject.meshMaternal Exposureen_HK
dc.subject.meshMilk, Human - chemistryen_HK
dc.subject.meshPopulation Surveillance - methodsen_HK
dc.subject.meshPregnancyen_HK
dc.titleAgreement between breast milk dioxin levels by CALUX bioassay and chemical analysis in a population survey in Hong Kongen_HK
dc.typeArticleen_HK
dc.identifier.emailHui, LL: huic@hkucc.hku.hken_HK
dc.identifier.emailHedley, AJ: hrmrajh@hkucc.hku.hken_HK
dc.identifier.emailCowling, BJ: bcowling@hku.hken_HK
dc.identifier.authorityHui, LL=rp01698en_HK
dc.identifier.authorityHedley, AJ=rp00357en_HK
dc.identifier.authorityCowling, BJ=rp01326en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chemosphere.2007.05.038en_HK
dc.identifier.pmid17618674-
dc.identifier.scopuseid_2-s2.0-34748831189en_HK
dc.identifier.hkuros138097-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34748831189&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume69en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1287en_HK
dc.identifier.epage1294en_HK
dc.identifier.isiWOS:000250426500014-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHui, LL=12774460100en_HK
dc.identifier.scopusauthoridHedley, AJ=7102584095en_HK
dc.identifier.scopusauthoridNelson, EAS=7402264387en_HK
dc.identifier.scopusauthoridMalisch, R=6701317539en_HK
dc.identifier.scopusauthoridWong, TW=7403531744en_HK
dc.identifier.scopusauthoridCowling, BJ=8644765500en_HK

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