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Article: Calcium binding to biliary mucins is dependent on sodium ion concentration: Relevance to cystic fibrosis

TitleCalcium binding to biliary mucins is dependent on sodium ion concentration: Relevance to cystic fibrosis
Authors
KeywordsBile
Bile ducts
Cystic fibrosis transmembrane conductance regulator
Gallbladder
Gallstones
Mucus
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2004, v. 314 n. 2, p. 330-334 How to Cite?
AbstractBecause hypersecretion of gallbladder (GB) mucus occurs in gallstone formation and because binding of Ca2+ to biliary lipids only accounts for 50% of the total Ca2+ in GB bile, we investigated the binding of Ca2+ to human biliary mucin. Biliary mucin was purified from GB bile and binding to Ca2+ studied. Scatchard plot analysis suggested two binding sites. Removal of sialic acid by neuraminidase resulted in 10% reduction of Ca2+ binding, whereas, sulfatase treatment reduced Ca2+ binding by 30%. Using a hypotonic NaCl solution, Ca 2+ binding to mucin increased curvilinearly with mucin concentration. However, binding decreased with increasing ionic strength of the NaCl solution. We conclude that binding of Ca2+ to mucin is effected mainly through sulfate. Binding to Ca2+ can be displaced by Na+. Ca2+ binding to mucins is enhanced in the setting of low Na + concentrations. This phenomenon has pathophysiologic implications for the formation of thick mucus in cystic fibrosis epithelia. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92531
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKuver, Ren_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:49:03Z-
dc.date.available2010-09-17T10:49:03Z-
dc.date.issued2004en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2004, v. 314 n. 2, p. 330-334en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92531-
dc.description.abstractBecause hypersecretion of gallbladder (GB) mucus occurs in gallstone formation and because binding of Ca2+ to biliary lipids only accounts for 50% of the total Ca2+ in GB bile, we investigated the binding of Ca2+ to human biliary mucin. Biliary mucin was purified from GB bile and binding to Ca2+ studied. Scatchard plot analysis suggested two binding sites. Removal of sialic acid by neuraminidase resulted in 10% reduction of Ca2+ binding, whereas, sulfatase treatment reduced Ca2+ binding by 30%. Using a hypotonic NaCl solution, Ca 2+ binding to mucin increased curvilinearly with mucin concentration. However, binding decreased with increasing ionic strength of the NaCl solution. We conclude that binding of Ca2+ to mucin is effected mainly through sulfate. Binding to Ca2+ can be displaced by Na+. Ca2+ binding to mucins is enhanced in the setting of low Na + concentrations. This phenomenon has pathophysiologic implications for the formation of thick mucus in cystic fibrosis epithelia. © 2003 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectBileen_HK
dc.subjectBile ductsen_HK
dc.subjectCystic fibrosis transmembrane conductance regulatoren_HK
dc.subjectGallbladderen_HK
dc.subjectGallstonesen_HK
dc.subjectMucusen_HK
dc.titleCalcium binding to biliary mucins is dependent on sodium ion concentration: Relevance to cystic fibrosisen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2003.12.088en_HK
dc.identifier.pmid14733909-
dc.identifier.scopuseid_2-s2.0-0345743624en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0345743624&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume314en_HK
dc.identifier.issue2en_HK
dc.identifier.spage330en_HK
dc.identifier.epage334en_HK
dc.identifier.isiWOS:000188612800005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKuver, R=6701723533en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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