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Article: Bidirectional transport of cholesterol between gallbladder epithelial cells and model bile

TitleBidirectional transport of cholesterol between gallbladder epithelial cells and model bile
Authors
Keywordsbile salt cytotoxicity
cholesterol absorption
cholesterol synthesis
Issue Date1996
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1996, v. 271 n. 3 34-3, p. G410-G414 How to Cite?
AbstractLipids in hepatic bile may be modified by the gallbladder epithelium. The purpose of this study was to demonstrate a bidirectional exchange of cholesterol between biliary lipid carriers and gallbladder epithelial cells and to determine the factors regulating this cholesterol transfer. (Gallbladder epithelial cells were cultured to confluent monolayers, their membranes were labeled with endogenously synthesized [14C]cholesterol, and the cells were incubated with model bile introduced into the apical membrane compartment. Similarly, model bile with different lipid composition containing [3H]cholesterol was incubated with the unlabeled monolayers. We found that cholesterol in the apical membrane bilayer of the epithelial cells exchanged readily with that in bile, but only in the presence of bile salts. The rate of exchange is dependent on the concentration and species of bile salts. The net gain of cholesterol (absorption) or net loss of cholesterol (cytotoxicity) exhibited by the epithelial cells was regulated by the thermodynamic stability of cholesterol and the detergent effect of mixed micelles in bile. It is also possible that the physicochemical composition of lipids in bile may modify the cellular function of the gallbladder epithelium.
Persistent Identifierhttp://hdl.handle.net/10722/92526
ISSN
2015 Impact Factor: 3.297
2015 SCImago Journal Rankings: 1.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHayashi, Aen_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:48:55Z-
dc.date.available2010-09-17T10:48:55Z-
dc.date.issued1996en_HK
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 1996, v. 271 n. 3 34-3, p. G410-G414en_HK
dc.identifier.issn0193-1857en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92526-
dc.description.abstractLipids in hepatic bile may be modified by the gallbladder epithelium. The purpose of this study was to demonstrate a bidirectional exchange of cholesterol between biliary lipid carriers and gallbladder epithelial cells and to determine the factors regulating this cholesterol transfer. (Gallbladder epithelial cells were cultured to confluent monolayers, their membranes were labeled with endogenously synthesized [14C]cholesterol, and the cells were incubated with model bile introduced into the apical membrane compartment. Similarly, model bile with different lipid composition containing [3H]cholesterol was incubated with the unlabeled monolayers. We found that cholesterol in the apical membrane bilayer of the epithelial cells exchanged readily with that in bile, but only in the presence of bile salts. The rate of exchange is dependent on the concentration and species of bile salts. The net gain of cholesterol (absorption) or net loss of cholesterol (cytotoxicity) exhibited by the epithelial cells was regulated by the thermodynamic stability of cholesterol and the detergent effect of mixed micelles in bile. It is also possible that the physicochemical composition of lipids in bile may modify the cellular function of the gallbladder epithelium.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_HK
dc.subjectbile salt cytotoxicityen_HK
dc.subjectcholesterol absorptionen_HK
dc.subjectcholesterol synthesisen_HK
dc.titleBidirectional transport of cholesterol between gallbladder epithelial cells and model bileen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8843763-
dc.identifier.scopuseid_2-s2.0-0029739067en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029739067&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume271en_HK
dc.identifier.issue3 34-3en_HK
dc.identifier.spageG410en_HK
dc.identifier.epageG414en_HK
dc.identifier.isiWOS:A1996VH51100004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHayashi, A=14022812400en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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