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Article: Mucous granule exocytosis and CFTR expression in gallbladder epithelium

TitleMucous granule exocytosis and CFTR expression in gallbladder epithelium
Authors
KeywordsCalcium
Chloride channel
Cystic fibrosis
Mucin
Issue Date2000
PublisherOxford University Press. The Journal's web site is located at http://glycob.oxfordjournals.org/
Citation
Glycobiology, 2000, v. 10 n. 2, p. 149-157 How to Cite?
AbstractA mechanistic model of mucous granule exocytosis by columnar epithelial cells must take into account the unique physical-chemical properties of mucin glycoproteins and the resultant mucus gel. In particular, any model must explain the intracellular packaging and the kinetics of release of these large, heavily charged species. We studied mucous granule exocytosis in gallbladder epithelium, a model system for mucus secretion by columnar epithelial cells. Mucous granules released mucus by merocrine exocytosis in mouse gallbladder epithelium when examined by transmission electron microscopy. Spherules of secreted mucus larger than intracellular granules were noted on scanning electron microscopy. Electron probe microanalysis demonstrated increased calcium concentrations within mucous granules. Immunofluorescence microscopic studies revealed intracellular colocalization of mucins and the cystic fibrosis transmembrane conductance regulator (CFTR). Confocal laser immunofluorescence microscopy confirmed colocalization. These observations suggest that calcium in mucous secretory granules provides cationic shielding to keep mucus tightly packed. The data also suggests CFTR chloride channels are present in granule membranes. These observations support a model in which influx of chloride ions into the granule disrupts cationic shielding, leading to rapid swelling, exocytosis and hydration of mucus. Such a model explains the physical-chemical mechanisms involved in mucous granule exocytosis.
Persistent Identifierhttp://hdl.handle.net/10722/92525
ISSN
2015 Impact Factor: 3.283
2015 SCImago Journal Rankings: 1.692
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKuver, Ren_HK
dc.contributor.authorKlinkspoor, JHen_HK
dc.contributor.authorOsborne, WRAen_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:48:53Z-
dc.date.available2010-09-17T10:48:53Z-
dc.date.issued2000en_HK
dc.identifier.citationGlycobiology, 2000, v. 10 n. 2, p. 149-157en_HK
dc.identifier.issn0959-6658en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92525-
dc.description.abstractA mechanistic model of mucous granule exocytosis by columnar epithelial cells must take into account the unique physical-chemical properties of mucin glycoproteins and the resultant mucus gel. In particular, any model must explain the intracellular packaging and the kinetics of release of these large, heavily charged species. We studied mucous granule exocytosis in gallbladder epithelium, a model system for mucus secretion by columnar epithelial cells. Mucous granules released mucus by merocrine exocytosis in mouse gallbladder epithelium when examined by transmission electron microscopy. Spherules of secreted mucus larger than intracellular granules were noted on scanning electron microscopy. Electron probe microanalysis demonstrated increased calcium concentrations within mucous granules. Immunofluorescence microscopic studies revealed intracellular colocalization of mucins and the cystic fibrosis transmembrane conductance regulator (CFTR). Confocal laser immunofluorescence microscopy confirmed colocalization. These observations suggest that calcium in mucous secretory granules provides cationic shielding to keep mucus tightly packed. The data also suggests CFTR chloride channels are present in granule membranes. These observations support a model in which influx of chloride ions into the granule disrupts cationic shielding, leading to rapid swelling, exocytosis and hydration of mucus. Such a model explains the physical-chemical mechanisms involved in mucous granule exocytosis.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://glycob.oxfordjournals.org/en_HK
dc.relation.ispartofGlycobiologyen_HK
dc.subjectCalciumen_HK
dc.subjectChloride channelen_HK
dc.subjectCystic fibrosisen_HK
dc.subjectMucinen_HK
dc.titleMucous granule exocytosis and CFTR expression in gallbladder epitheliumen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10642606-
dc.identifier.scopuseid_2-s2.0-0034027661en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034027661&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issue2en_HK
dc.identifier.spage149en_HK
dc.identifier.epage157en_HK
dc.identifier.isiWOS:000085143700004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKuver, R=6701723533en_HK
dc.identifier.scopusauthoridKlinkspoor, JH=6602590656en_HK
dc.identifier.scopusauthoridOsborne, WRA=7005020110en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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