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Article: Effects of bile salts on cholestan-3β,5α,6β-triol-induced apoptosis in dog gallbladder epithelial cells

TitleEffects of bile salts on cholestan-3β,5α,6β-triol-induced apoptosis in dog gallbladder epithelial cells
Authors
KeywordsApoptosis
Bile salt
Biliary tract
Gall bladder
Oxysterol
Issue Date2001
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbalip
Citation
Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids, 2001, v. 1530 n. 2-3, p. 199-208 How to Cite?
AbstractOxysterols are cytotoxic agents. The gallbladder epithelium is exposed to high concentrations of oxysterols, and so elucidating the mechanisms of cytotoxicity in this organ may enhance our understanding of the pathogenesis of biliary tract disorders. We investigated the cytotoxic effects of the oxysterol cholestan-3β,5α,6β-triol (TriolC) on dog gallbladder epithelial cells. Apoptosis was the major form of cytotoxicity, as determined by analysis of nuclear morphologic changes and by multiparameter flow cytometry. Hydrophobic bile salts are known to have cytotoxic effects, whereas hydrophilic bile salts have cytoprotective effects. We therefore examined whether the hydrophobic bile acid taurodeoxycholic acid (TDC) and the hydrophilic bile acid tauroursodeoxycholic acid (TUDC) had modifying effects on oxysterol-induced cytotoxicity. TriolC caused an increase in the number of apoptotic cells from 14 ± 11% (control) to 48 ± 12% of total cells (P < 0.01). After combining TriolC with TDC, cell apoptosis increased to 63 ± 16% (P < 0.05), whereas after addition of TUDC, the number of apoptotic cells decreased to 31 ± 12% (P < 0.05) of total cells. In summary, oxysterols such as TriolC induce apoptosis. Hydrophobic bile salts enhance TriolC-induced apoptosis, whereas hydrophilic bile salts diminish TriolC-induced apoptosis. These results suggest that interactions between oxysterols and bile salts play a role in the pathophysiology of biliary tract disorders. © 2001 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/92522
ISSN
2015 Impact Factor: 4.779
2015 SCImago Journal Rankings: 2.467
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYoshida, Ten_HK
dc.contributor.authorKlinkspoor, JHen_HK
dc.contributor.authorKuver, Ren_HK
dc.contributor.authorPoot, Men_HK
dc.contributor.authorRabinovitch, PSen_HK
dc.contributor.authorWrenn, SPen_HK
dc.contributor.authorKaler, EWen_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:48:47Z-
dc.date.available2010-09-17T10:48:47Z-
dc.date.issued2001en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids, 2001, v. 1530 n. 2-3, p. 199-208en_HK
dc.identifier.issn1388-1981en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92522-
dc.description.abstractOxysterols are cytotoxic agents. The gallbladder epithelium is exposed to high concentrations of oxysterols, and so elucidating the mechanisms of cytotoxicity in this organ may enhance our understanding of the pathogenesis of biliary tract disorders. We investigated the cytotoxic effects of the oxysterol cholestan-3β,5α,6β-triol (TriolC) on dog gallbladder epithelial cells. Apoptosis was the major form of cytotoxicity, as determined by analysis of nuclear morphologic changes and by multiparameter flow cytometry. Hydrophobic bile salts are known to have cytotoxic effects, whereas hydrophilic bile salts have cytoprotective effects. We therefore examined whether the hydrophobic bile acid taurodeoxycholic acid (TDC) and the hydrophilic bile acid tauroursodeoxycholic acid (TUDC) had modifying effects on oxysterol-induced cytotoxicity. TriolC caused an increase in the number of apoptotic cells from 14 ± 11% (control) to 48 ± 12% of total cells (P < 0.01). After combining TriolC with TDC, cell apoptosis increased to 63 ± 16% (P < 0.05), whereas after addition of TUDC, the number of apoptotic cells decreased to 31 ± 12% (P < 0.05) of total cells. In summary, oxysterols such as TriolC induce apoptosis. Hydrophobic bile salts enhance TriolC-induced apoptosis, whereas hydrophilic bile salts diminish TriolC-induced apoptosis. These results suggest that interactions between oxysterols and bile salts play a role in the pathophysiology of biliary tract disorders. © 2001 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbalipen_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipidsen_HK
dc.subjectApoptosisen_HK
dc.subjectBile salten_HK
dc.subjectBiliary tracten_HK
dc.subjectGall bladderen_HK
dc.subjectOxysterolen_HK
dc.titleEffects of bile salts on cholestan-3β,5α,6β-triol-induced apoptosis in dog gallbladder epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1388-1981(00)00183-9en_HK
dc.identifier.pmid11239822en_HK
dc.identifier.scopuseid_2-s2.0-0035952337en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035952337&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1530en_HK
dc.identifier.issue2-3en_HK
dc.identifier.spage199en_HK
dc.identifier.epage208en_HK
dc.identifier.isiWOS:000167372600008-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridYoshida, T=7501317704en_HK
dc.identifier.scopusauthoridKlinkspoor, JH=6602590656en_HK
dc.identifier.scopusauthoridKuver, R=6701723533en_HK
dc.identifier.scopusauthoridPoot, M=7006476750en_HK
dc.identifier.scopusauthoridRabinovitch, PS=7006580417en_HK
dc.identifier.scopusauthoridWrenn, SP=6603940041en_HK
dc.identifier.scopusauthoridKaler, EW=7007157989en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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