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Article: Characterization of two distinct chloride channels in cultured dog pancreatic duct epithelial cells

TitleCharacterization of two distinct chloride channels in cultured dog pancreatic duct epithelial cells
Authors
Keywords4,4'- diisothiocyanostilbene-2,2'-disulfonic acid
5- nitro-2-(3-phenylpropylamino)benzoic acid
cystic fibrosis
cystic fibrosis transmembrane conductance regulator
diphenylamine-2 carboxylate
immunoblot
iodide efflux
pancreatic exocrine secretion
patch-clamp technique
whole cell recording
Issue Date1997
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1997, v. 272 n. 1 35-1, p. G172-G180 How to Cite?
AbstractCl- secretion by pancreatic duct epithelial cells (PDEC) regulates cellular HCO3 secretion, an important component of the exocrine pancreas. In cystic fibrosis, for example, impaired function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel results in decreased pancreatic secretion and secondary pancreatic insufficiency. Studies of ion transport by PDEC have been hindered by the lack of a practical in vitro model. We have successfully cultured nontransformed dog PDEC on Vitrogen- coated permeable membranes overlying a feeder layer of myofibroblasts and report the characterization of Cl- channels in these cells. Cl- conductance, assessed through efflux of 125I from PDEC, was stimulated by agents acting via adenosine 3',5'-cyclic monophosphate (cAMP) or cytosolic Ca2+. The Cl- conductances activated by cAMP and Ca2+ were distinct, since they were differentially inhibited by 4,4'-diisothiocyanostilbene- 2,2'-disulfonic acid and, to a lesser extent, by 5-nitro-2-(3- phenylpropylamino)benzoic acid and diphenylamine-2 carboxylate. Patch-clamp studies confirmed the presence of Cl- channels activated by cAMP and Ca2+, with differential inhibition by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The presence of CFTR Cl- channels in PDEC was confirmed by immunoblotting. These cultured PDEC are an optimal model for studies of pancreatic duct secretion.
Persistent Identifierhttp://hdl.handle.net/10722/92509
ISSN
2015 Impact Factor: 3.297
2015 SCImago Journal Rankings: 1.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNguyen, TDen_HK
dc.contributor.authorKoh, DSen_HK
dc.contributor.authorMoody, MWen_HK
dc.contributor.authorFox, NRen_HK
dc.contributor.authorSavard, CEen_HK
dc.contributor.authorKuver, Ren_HK
dc.contributor.authorHille, Ben_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:48:24Z-
dc.date.available2010-09-17T10:48:24Z-
dc.date.issued1997en_HK
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 1997, v. 272 n. 1 35-1, p. G172-G180en_HK
dc.identifier.issn0193-1857en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92509-
dc.description.abstractCl- secretion by pancreatic duct epithelial cells (PDEC) regulates cellular HCO3 secretion, an important component of the exocrine pancreas. In cystic fibrosis, for example, impaired function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel results in decreased pancreatic secretion and secondary pancreatic insufficiency. Studies of ion transport by PDEC have been hindered by the lack of a practical in vitro model. We have successfully cultured nontransformed dog PDEC on Vitrogen- coated permeable membranes overlying a feeder layer of myofibroblasts and report the characterization of Cl- channels in these cells. Cl- conductance, assessed through efflux of 125I from PDEC, was stimulated by agents acting via adenosine 3',5'-cyclic monophosphate (cAMP) or cytosolic Ca2+. The Cl- conductances activated by cAMP and Ca2+ were distinct, since they were differentially inhibited by 4,4'-diisothiocyanostilbene- 2,2'-disulfonic acid and, to a lesser extent, by 5-nitro-2-(3- phenylpropylamino)benzoic acid and diphenylamine-2 carboxylate. Patch-clamp studies confirmed the presence of Cl- channels activated by cAMP and Ca2+, with differential inhibition by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The presence of CFTR Cl- channels in PDEC was confirmed by immunoblotting. These cultured PDEC are an optimal model for studies of pancreatic duct secretion.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_HK
dc.subject4,4'- diisothiocyanostilbene-2,2'-disulfonic aciden_HK
dc.subject5- nitro-2-(3-phenylpropylamino)benzoic aciden_HK
dc.subjectcystic fibrosisen_HK
dc.subjectcystic fibrosis transmembrane conductance regulatoren_HK
dc.subjectdiphenylamine-2 carboxylateen_HK
dc.subjectimmunobloten_HK
dc.subjectiodide effluxen_HK
dc.subjectpancreatic exocrine secretionen_HK
dc.subjectpatch-clamp techniqueen_HK
dc.subjectwhole cell recordingen_HK
dc.titleCharacterization of two distinct chloride channels in cultured dog pancreatic duct epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9038891en_HK
dc.identifier.scopuseid_2-s2.0-0031035256en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031035256&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume272en_HK
dc.identifier.issue1 35-1en_HK
dc.identifier.spageG172en_HK
dc.identifier.epageG180en_HK
dc.identifier.isiWOS:A1997WG27100024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNguyen, TD=35546959700en_HK
dc.identifier.scopusauthoridKoh, DS=36965287000en_HK
dc.identifier.scopusauthoridMoody, MW=7102854884en_HK
dc.identifier.scopusauthoridFox, NR=7201444384en_HK
dc.identifier.scopusauthoridSavard, CE=6701738621en_HK
dc.identifier.scopusauthoridKuver, R=6701723533en_HK
dc.identifier.scopusauthoridHille, B=7005667943en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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