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Article: Effects of lipopolysaccharide on platelet-derived growth factor isoform and receptor expression in cultured rat common bile duct fibroblasts and cholangiocytes

TitleEffects of lipopolysaccharide on platelet-derived growth factor isoform and receptor expression in cultured rat common bile duct fibroblasts and cholangiocytes
Authors
KeywordsCholangitis
Extrahepatic biliary system
Fibrosis
Issue Date2009
PublisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JGH
Citation
Journal Of Gastroenterology And Hepatology, 2009, v. 24 n. 7, p. 1218-1225 How to Cite?
AbstractBackground and Aim: Little is known about the role of platelet-derived growth factor (PDGF) in biliary fibrosis in the setting of bacterial colonization of the biliary tree. We therefore sought to investigate whether exposure to bacterial lipopolysaccharide (LPS) alters PDGF isoform and receptor expression in cultured rat common bile duct fibroblasts (CBDF) and normal rat cholangiocytes (NRC). Methods: Collagen content in cells and media was assessed by colorimetric assay and gel electrophoresis. mRNA levels of PDGF-A and -B, and PDGF-Receptors (PDGF-R) α and β were measured by relative quantitative real-time PCR. Protein levels of PDGF-AA, AB and BB were measured by ELISA, and PDGF-Rα and PDGF-Rβ by Western blot. Results: In CBDF, LPS increased total soluble collagen synthesis and secretion. PDGF-Rα and β mRNA and protein were also increased by LPS treatment in CBDF. Lipopolysaccharide treatment elicited an increase in PDGF-A and -B mRNA levels in CBDF. In NRC, levels of PDGF-A mRNA increased in a dose-dependent fashion following LPS treatment, whereas PDGF-B mRNA showed no response. PDGF-AA secretion was higher by CBDF than by NRC. PDGF-BB levels were also higher in CBDF than in NRC. While PDGF-BB levels did not respond to LPS treatment in CBDF, there was a dose-dependent response of this isoform to LPS in NRC. Intracellular and secreted PDGF-AB increased with LPS treatment in NRC. Conclusions: These results support a model in which chronic bacterial colonization of the biliary tree induces fibrosis through PDGF-dependent mechanisms. © 2007 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/92504
ISSN
2015 Impact Factor: 3.322
2015 SCImago Journal Rankings: 1.190
ISI Accession Number ID
Funding AgencyGrant Number
Merit Review Award from the Department of Veterans Affairs
Wonkwang Institute of Clinical Medicine, Iksan, South Korea
Funding Information:

We thank J. Donald Ostrow for critical reading of the manuscript. This work was supported by a Merit Review Award from the Department of Veterans Affairs. Dr. Tae-Hyeon Kim was supported by the Wonkwang Institute of Clinical Medicine, Iksan, South Korea.

References

 

DC FieldValueLanguage
dc.contributor.authorKim, THen_HK
dc.contributor.authorMoon, JHen_HK
dc.contributor.authorSavard, CEen_HK
dc.contributor.authorKuver, Ren_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:48:15Z-
dc.date.available2010-09-17T10:48:15Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Gastroenterology And Hepatology, 2009, v. 24 n. 7, p. 1218-1225en_HK
dc.identifier.issn0815-9319en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92504-
dc.description.abstractBackground and Aim: Little is known about the role of platelet-derived growth factor (PDGF) in biliary fibrosis in the setting of bacterial colonization of the biliary tree. We therefore sought to investigate whether exposure to bacterial lipopolysaccharide (LPS) alters PDGF isoform and receptor expression in cultured rat common bile duct fibroblasts (CBDF) and normal rat cholangiocytes (NRC). Methods: Collagen content in cells and media was assessed by colorimetric assay and gel electrophoresis. mRNA levels of PDGF-A and -B, and PDGF-Receptors (PDGF-R) α and β were measured by relative quantitative real-time PCR. Protein levels of PDGF-AA, AB and BB were measured by ELISA, and PDGF-Rα and PDGF-Rβ by Western blot. Results: In CBDF, LPS increased total soluble collagen synthesis and secretion. PDGF-Rα and β mRNA and protein were also increased by LPS treatment in CBDF. Lipopolysaccharide treatment elicited an increase in PDGF-A and -B mRNA levels in CBDF. In NRC, levels of PDGF-A mRNA increased in a dose-dependent fashion following LPS treatment, whereas PDGF-B mRNA showed no response. PDGF-AA secretion was higher by CBDF than by NRC. PDGF-BB levels were also higher in CBDF than in NRC. While PDGF-BB levels did not respond to LPS treatment in CBDF, there was a dose-dependent response of this isoform to LPS in NRC. Intracellular and secreted PDGF-AB increased with LPS treatment in NRC. Conclusions: These results support a model in which chronic bacterial colonization of the biliary tree induces fibrosis through PDGF-dependent mechanisms. © 2007 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JGHen_HK
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.subjectCholangitisen_HK
dc.subjectExtrahepatic biliary systemen_HK
dc.subjectFibrosisen_HK
dc.titleEffects of lipopolysaccharide on platelet-derived growth factor isoform and receptor expression in cultured rat common bile duct fibroblasts and cholangiocytesen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2008.05729.xen_HK
dc.identifier.pmid19691150-
dc.identifier.scopuseid_2-s2.0-68749122256en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68749122256&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1218en_HK
dc.identifier.epage1225en_HK
dc.identifier.eissn1440-1746-
dc.identifier.isiWOS:000268456900013-
dc.publisher.placeAustraliaen_HK
dc.identifier.scopusauthoridKim, TH=36062469400en_HK
dc.identifier.scopusauthoridMoon, JH=55183387700en_HK
dc.identifier.scopusauthoridSavard, CE=6701738621en_HK
dc.identifier.scopusauthoridKuver, R=6701723533en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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