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Article: Gallbladder epithelial cells that engraft in mouse liver can differentiate into hepatocyte-like cells

TitleGallbladder epithelial cells that engraft in mouse liver can differentiate into hepatocyte-like cells
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2009
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 2009, v. 174 n. 3, p. 842-853 How to Cite?
AbstractWe tested the hypothesis that well-differentiated gallbladder epithelial cells (GBECs) are capable of engrafting and surviving in murine liver and acquire phenotypic characteristics of hepatocytes. GBECs isolated from transgenic mice that constitutively express green fluorescent protein (GFP) were either cultured before transplantation or transplanted immediately following isolation. Recipient mice with severe-combined immunodeficiency underwent retrorsine treatment and either partial hepatectomy before transplantation or carbon tetrachloride treatment following transplantation. From 1 to 4 months following transplantation, the livers of recipient mice contained discrete colonies of GFP+ cells. Most GFP+ cells surrounded vesicles, were epithelial cell-like in morphology, and expressed the biliary epithelial markers cytokeratin 19 and carbonic anhydrase IV. Subpopulations of GFP+ cells resembled hepatocytes morphologically and expressed the hepatocyte-specific markers connexin-32 and hepatic nuclear factor-4α, but not cytokeratin 19 or carbonic anhydrase IV. At 4 months, cells in GFP + colonies were not actively proliferating as determined by proliferating cell nuclear antigen expression. Thus, GBECs are capable of engrafting and surviving in damaged mouse livers, and some can differentiate into cells with hepatocyte-like features. These findings suggest that environmental cues in the recipient liver are sufficient to allow a subpopulation of donor GBECs to differentiate into hepatocyte-like cells in the absence of exogenous transcriptional reprogramming. GBECs might be used as donor cells in a cell transplantation approach for the treatment of liver disease. Copyright © American Society for Investigative Pathology.
Persistent Identifierhttp://hdl.handle.net/10722/92503
ISSN
2015 Impact Factor: 4.206
2015 SCImago Journal Rankings: 2.653
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Institute of Diabetes and Digestive and Kidney DiseasesDK-061157
Department of Veterans Affairs
Funding Information:

Supported by Grant DK-061157 from the National Institute of Diabetes and Digestive and Kidney Diseases and a Merit Review Award from the Department of Veterans Affairs.

References

 

DC FieldValueLanguage
dc.contributor.authorLee, SPen_HK
dc.contributor.authorSavard, CEen_HK
dc.contributor.authorKuver, Ren_HK
dc.date.accessioned2010-09-17T10:48:14Z-
dc.date.available2010-09-17T10:48:14Z-
dc.date.issued2009en_HK
dc.identifier.citationAmerican Journal Of Pathology, 2009, v. 174 n. 3, p. 842-853en_HK
dc.identifier.issn0002-9440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92503-
dc.description.abstractWe tested the hypothesis that well-differentiated gallbladder epithelial cells (GBECs) are capable of engrafting and surviving in murine liver and acquire phenotypic characteristics of hepatocytes. GBECs isolated from transgenic mice that constitutively express green fluorescent protein (GFP) were either cultured before transplantation or transplanted immediately following isolation. Recipient mice with severe-combined immunodeficiency underwent retrorsine treatment and either partial hepatectomy before transplantation or carbon tetrachloride treatment following transplantation. From 1 to 4 months following transplantation, the livers of recipient mice contained discrete colonies of GFP+ cells. Most GFP+ cells surrounded vesicles, were epithelial cell-like in morphology, and expressed the biliary epithelial markers cytokeratin 19 and carbonic anhydrase IV. Subpopulations of GFP+ cells resembled hepatocytes morphologically and expressed the hepatocyte-specific markers connexin-32 and hepatic nuclear factor-4α, but not cytokeratin 19 or carbonic anhydrase IV. At 4 months, cells in GFP + colonies were not actively proliferating as determined by proliferating cell nuclear antigen expression. Thus, GBECs are capable of engrafting and surviving in damaged mouse livers, and some can differentiate into cells with hepatocyte-like features. These findings suggest that environmental cues in the recipient liver are sufficient to allow a subpopulation of donor GBECs to differentiate into hepatocyte-like cells in the absence of exogenous transcriptional reprogramming. GBECs might be used as donor cells in a cell transplantation approach for the treatment of liver disease. Copyright © American Society for Investigative Pathology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_HK
dc.relation.ispartofAmerican Journal of Pathologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleGallbladder epithelial cells that engraft in mouse liver can differentiate into hepatocyte-like cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2353/ajpath.2009.080262en_HK
dc.identifier.pmid19218347-
dc.identifier.pmcidPMC2665745-
dc.identifier.scopuseid_2-s2.0-62549150730en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62549150730&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume174en_HK
dc.identifier.issue3en_HK
dc.identifier.spage842en_HK
dc.identifier.epage853en_HK
dc.identifier.eissn1525-2191-
dc.identifier.isiWOS:000263612600013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK
dc.identifier.scopusauthoridSavard, CE=6701738621en_HK
dc.identifier.scopusauthoridKuver, R=6701723533en_HK

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