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Article: Dog pancreatic duct epithelial cells: Long-term culture and characterization

TitleDog pancreatic duct epithelial cells: Long-term culture and characterization
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1996
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 1996, v. 148 n. 3, p. 977-985 How to Cite?
AbstractEpithelial cells, isolated front a normal dog pancreatic duct, were grown on collagen-coated culture inserts suspended above a feeder layer of myofibroblasts. The cells were examined by transmission electron microscopy, immunohistochemistry, cytogenetics, and flow cytometry. In addition, the constitutive and agonist-stimulated mucin secretion of these cells was studied using a [3H]N-acetyl-D-glucosamine labeling assay, and the stimulation of intracellular cAMP was measured. Cells grown on inserts with a feeder layer developed into confluent monolayers consisting of strictly polarized columnar epithelial cells with prominent microvilli, intercellular junctions, and normal chromosomal characteristics. They could be passaged repeatedly without a detectable alteration in their morphology. The cells could also be grown on organotypic cultures, resulting in further differentiated cells simulating in vivo morphology. Immunohistochemistry demonstrated the presence of carbonic anhydrase II in these cells. Cells treated with vasoactive intestinal peptide, epinephrine, and dibutyryl-cAMP demonstrated a marked increase in mucin secretion compared with controls. In parallel experiments, VIP and epinephrine significantly increased intracellular cAMP. In conclusion we have developed a pancreatic epithelial cell preparation with morphology, cytokinetics, chromosomal, and DNA analyses characteristic of normal cells. Similar to normal columnar epithelial cells, these pancreatic duct cells secreted mucin constitutively and responded to agonist by increasing secretion via a cAMP-mediated pathway. They also contained carbonic anhydrase, which indicates that the cells are capable of secreting bicarbonate.
Persistent Identifierhttp://hdl.handle.net/10722/92498
ISSN
2015 Impact Factor: 4.206
2015 SCImago Journal Rankings: 2.653
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorOda, Den_HK
dc.contributor.authorSavard, CEen_HK
dc.contributor.authorNguyen, TDen_HK
dc.contributor.authorEng, Len_HK
dc.contributor.authorSwenson, ERen_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:48:05Z-
dc.date.available2010-09-17T10:48:05Z-
dc.date.issued1996en_HK
dc.identifier.citationAmerican Journal Of Pathology, 1996, v. 148 n. 3, p. 977-985en_HK
dc.identifier.issn0002-9440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92498-
dc.description.abstractEpithelial cells, isolated front a normal dog pancreatic duct, were grown on collagen-coated culture inserts suspended above a feeder layer of myofibroblasts. The cells were examined by transmission electron microscopy, immunohistochemistry, cytogenetics, and flow cytometry. In addition, the constitutive and agonist-stimulated mucin secretion of these cells was studied using a [3H]N-acetyl-D-glucosamine labeling assay, and the stimulation of intracellular cAMP was measured. Cells grown on inserts with a feeder layer developed into confluent monolayers consisting of strictly polarized columnar epithelial cells with prominent microvilli, intercellular junctions, and normal chromosomal characteristics. They could be passaged repeatedly without a detectable alteration in their morphology. The cells could also be grown on organotypic cultures, resulting in further differentiated cells simulating in vivo morphology. Immunohistochemistry demonstrated the presence of carbonic anhydrase II in these cells. Cells treated with vasoactive intestinal peptide, epinephrine, and dibutyryl-cAMP demonstrated a marked increase in mucin secretion compared with controls. In parallel experiments, VIP and epinephrine significantly increased intracellular cAMP. In conclusion we have developed a pancreatic epithelial cell preparation with morphology, cytokinetics, chromosomal, and DNA analyses characteristic of normal cells. Similar to normal columnar epithelial cells, these pancreatic duct cells secreted mucin constitutively and responded to agonist by increasing secretion via a cAMP-mediated pathway. They also contained carbonic anhydrase, which indicates that the cells are capable of secreting bicarbonate.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_HK
dc.relation.ispartofAmerican Journal of Pathologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleDog pancreatic duct epithelial cells: Long-term culture and characterizationen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8774152-
dc.identifier.scopuseid_2-s2.0-0030092046en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030092046&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume148en_HK
dc.identifier.issue3en_HK
dc.identifier.spage977en_HK
dc.identifier.epage985en_HK
dc.identifier.isiWOS:A1996TY07400031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridOda, D=7006186359en_HK
dc.identifier.scopusauthoridSavard, CE=6701738621en_HK
dc.identifier.scopusauthoridNguyen, TD=35546959700en_HK
dc.identifier.scopusauthoridEng, L=25942882000en_HK
dc.identifier.scopusauthoridSwenson, ER=7102984915en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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