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Article: Isolation and long-term culture of gallbladder epithelial cells from wild-type and CF mice

TitleIsolation and long-term culture of gallbladder epithelial cells from wild-type and CF mice
Authors
Keywordscell culture
CFTR
cystic fibrosis
epithelium
gallbladder
murine
Issue Date1997
PublisherSpringer New York LLC. The Journal's web site is located at http://www.sivb.org/pubs_a_index.asp
Citation
In Vitro Cellular And Developmental Biology - Animal, 1997, v. 33 n. 2, p. 104-109 How to Cite?
AbstractMice with targeted disruption of the cftr gene show pathophysiologic changes in the gallbladder, which correlate with hepatobiliary disease seen in cystic fibrosis patients. As gallbladder epithelium secretes mucin, and as this epithelium consists of a relatively homogenous cell type, study of CFTR function in these cells would be beneficial to delineate the complex cellular functions of this protein. The size and anatomic location of the murine gallbladder makes such studies difficult in vivo. Therefore, the need exits for in vitro models of gallbladder epithelium. We describe a method to isolate and culture murine gallbladder epithelium from wild-type and CF mice. Cells were grown in a monolayer on porous inserts over a feeder layer of fibroblasts. These nontransformed cells can be successively passaged and maintain a well-differentiated epithelial cell phenotype as shown by morphologic criteria, characterized by polarized columnar epithelial cells with prominent microvilli and intercellular junctions. Organotypic cultures showed columnar cells simulating in vivo morphology. This culture system should be valuable in delineating cellular processes relating to CFTR in gallbladder epithelium.
Persistent Identifierhttp://hdl.handle.net/10722/92492
ISSN
2015 Impact Factor: 0.971
2015 SCImago Journal Rankings: 0.544
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKuver, Ren_HK
dc.contributor.authorSavard, Cen_HK
dc.contributor.authorNguyen, TDen_HK
dc.contributor.authorOsborne, WRAen_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:47:54Z-
dc.date.available2010-09-17T10:47:54Z-
dc.date.issued1997en_HK
dc.identifier.citationIn Vitro Cellular And Developmental Biology - Animal, 1997, v. 33 n. 2, p. 104-109en_HK
dc.identifier.issn1071-2690en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92492-
dc.description.abstractMice with targeted disruption of the cftr gene show pathophysiologic changes in the gallbladder, which correlate with hepatobiliary disease seen in cystic fibrosis patients. As gallbladder epithelium secretes mucin, and as this epithelium consists of a relatively homogenous cell type, study of CFTR function in these cells would be beneficial to delineate the complex cellular functions of this protein. The size and anatomic location of the murine gallbladder makes such studies difficult in vivo. Therefore, the need exits for in vitro models of gallbladder epithelium. We describe a method to isolate and culture murine gallbladder epithelium from wild-type and CF mice. Cells were grown in a monolayer on porous inserts over a feeder layer of fibroblasts. These nontransformed cells can be successively passaged and maintain a well-differentiated epithelial cell phenotype as shown by morphologic criteria, characterized by polarized columnar epithelial cells with prominent microvilli and intercellular junctions. Organotypic cultures showed columnar cells simulating in vivo morphology. This culture system should be valuable in delineating cellular processes relating to CFTR in gallbladder epithelium.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.sivb.org/pubs_a_index.aspen_HK
dc.relation.ispartofIn Vitro Cellular and Developmental Biology - Animalen_HK
dc.subjectcell cultureen_HK
dc.subjectCFTRen_HK
dc.subjectcystic fibrosisen_HK
dc.subjectepitheliumen_HK
dc.subjectgallbladderen_HK
dc.subjectmurineen_HK
dc.titleIsolation and long-term culture of gallbladder epithelial cells from wild-type and CF miceen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9081217-
dc.identifier.scopuseid_2-s2.0-0030892737en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030892737&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue2en_HK
dc.identifier.spage104en_HK
dc.identifier.epage109en_HK
dc.identifier.isiWOS:A1997WP52900007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKuver, R=6701723533en_HK
dc.identifier.scopusauthoridSavard, C=6701738621en_HK
dc.identifier.scopusauthoridNguyen, TD=35546959700en_HK
dc.identifier.scopusauthoridOsborne, WRA=7005020110en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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