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Article: Secretory effects of ATP on nontransformed dog pancreatic duct epithelial cells

TitleSecretory effects of ATP on nontransformed dog pancreatic duct epithelial cells
Authors
KeywordsAdenosine 5'-triphosphate
Chloride channels
Cystic fibrosis
Mucin
Potassium channels
Short-circuit current
Issue Date1998
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1998, v. 275 n. 1 38-1, p. G104-G11 How to Cite?
AbstractExtracellular triphosphate nucleotides, such as ATP, may regulate various cellular functions through specific cell surface receptors. We examine in this report the different secretory effects of ATP and analogs on nontransformed dog pancreatic duct epithelial cells (PDEC). We observed that 1) ATP, UTP, adenosine 5'-O-(3-thiotriphosphate), and, to a lesser extent, β,γ-methylene-ATP, but not adenosine, stimulated 125I- efflux from PDEC, suggesting a primary role for P(2Y2) receptors, 2) ATP-stimulated 125I- efflux was inhibited by 5-nitro-2-(3-phenylpropylamino)benzoic acid, diphenylamine-2-carboxylate, and DIDS, suggesting mediation through Ca 2+- activated Cl - channels, 3) ATP stimulated an 86Rb + efflux sensitive to BaCl 2 and charybdotoxin, thus likely occurring through Ca 2+-activated K + channels, 4) serosal or luminal addition of UTP activated apical Cl - conductance and basolateral K + conductance when nystatin-permeabilized PDEC were studied in an Ussing chamber, suggesting the expression of P(2Y2) receptors on both sides of the cell, 5) ATP stimulated mucin secretion, and 6) ATP increases intracellular Ca 2+ concentration ([Ca 2+](i)). In conclusion, ATP and UTP interact with P(2Y2) receptors on nontransformed PDEC to increase [Ca 2+](i), stimulate mucin secretion, and activate ion conductances; these findings have implications for pancreatic exocrine function in both health and disease, such as cystic fibrosis.
Persistent Identifierhttp://hdl.handle.net/10722/92484
ISSN
2015 Impact Factor: 3.297
2015 SCImago Journal Rankings: 1.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNguyen, TDen_HK
dc.contributor.authorMoody, MWen_HK
dc.contributor.authorSavard, CEen_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:47:40Z-
dc.date.available2010-09-17T10:47:40Z-
dc.date.issued1998en_HK
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 1998, v. 275 n. 1 38-1, p. G104-G11en_HK
dc.identifier.issn0193-1857en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92484-
dc.description.abstractExtracellular triphosphate nucleotides, such as ATP, may regulate various cellular functions through specific cell surface receptors. We examine in this report the different secretory effects of ATP and analogs on nontransformed dog pancreatic duct epithelial cells (PDEC). We observed that 1) ATP, UTP, adenosine 5'-O-(3-thiotriphosphate), and, to a lesser extent, β,γ-methylene-ATP, but not adenosine, stimulated 125I- efflux from PDEC, suggesting a primary role for P(2Y2) receptors, 2) ATP-stimulated 125I- efflux was inhibited by 5-nitro-2-(3-phenylpropylamino)benzoic acid, diphenylamine-2-carboxylate, and DIDS, suggesting mediation through Ca 2+- activated Cl - channels, 3) ATP stimulated an 86Rb + efflux sensitive to BaCl 2 and charybdotoxin, thus likely occurring through Ca 2+-activated K + channels, 4) serosal or luminal addition of UTP activated apical Cl - conductance and basolateral K + conductance when nystatin-permeabilized PDEC were studied in an Ussing chamber, suggesting the expression of P(2Y2) receptors on both sides of the cell, 5) ATP stimulated mucin secretion, and 6) ATP increases intracellular Ca 2+ concentration ([Ca 2+](i)). In conclusion, ATP and UTP interact with P(2Y2) receptors on nontransformed PDEC to increase [Ca 2+](i), stimulate mucin secretion, and activate ion conductances; these findings have implications for pancreatic exocrine function in both health and disease, such as cystic fibrosis.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_HK
dc.subjectAdenosine 5'-triphosphateen_HK
dc.subjectChloride channelsen_HK
dc.subjectCystic fibrosisen_HK
dc.subjectMucinen_HK
dc.subjectPotassium channelsen_HK
dc.subjectShort-circuit currenten_HK
dc.titleSecretory effects of ATP on nontransformed dog pancreatic duct epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9655690-
dc.identifier.scopuseid_2-s2.0-0031857928en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031857928&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume275en_HK
dc.identifier.issue1 38-1en_HK
dc.identifier.spageG104en_HK
dc.identifier.epageG11en_HK
dc.identifier.isiWOS:000074725200014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNguyen, TD=35546959700en_HK
dc.identifier.scopusauthoridMoody, MW=7102854884en_HK
dc.identifier.scopusauthoridSavard, CE=6701738621en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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