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Article: Detection and characterization of laterally phase separated cholesterol domains in model lipid membranes
Title | Detection and characterization of laterally phase separated cholesterol domains in model lipid membranes |
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Authors | |
Keywords | Bilayer Cholesterol DMPC Domains Liquid-ordered |
Issue Date | 2003 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/colsurfb |
Citation | Colloids And Surfaces B: Biointerfaces, 2003, v. 29 n. 2-3, p. 217-231 How to Cite? |
Abstract | We present evidence that laterally phase separated cholesterol domains constitute a new, equilibrium phase in biological membranes. The domains are characterized in multi-lamellar vesicles (MLV) made of cholesterol and dimyristoylphosphatidylcholine (DMPC) but are also shown to exist in biologically relevant, egg lecithin systems containing a mixture of phospholipids. This work utilizes the fluorescent membrane probes 1-acyl-2-[12-[(5-dimethylamino-1-naphthalenesufonyl)amino]dodecanoyl]-sn- glycero-3-phosphocholine (DANSYL), and ergosta-5,7,9(11),22-tetraen-3β-ol (ERGO), which have been shown to be minimally invasive mimics of native membrane lipids. The highlight of the work is a heating-induced alleviation of a DANSYL blue shift at relatively high (but undersaturated) cholesterol loadings, which is reversible through at least three heating and cooling cycles. Comparison of the DANSYL spectral shifts with published DMPC-cholesterol phase diagrams shows unequivocally that the spectral results cannot be explained in terms of previously understood phase behavior. Rather, a lateral phase separation occurs within the vesicle bilayer, giving rise to cholesterol micro-domains. The cholesterol domains appear to coexist with, and should not be confused with, the well-known liquid-order phase that arises because of the cholesterol condensation effect. Additional studies involving ERGO-DANSYL energy transfer show a sequestration of probes within the bilayer, confirming the DANSYL spectral data, and a model that includes domains provides the best description of measured energy transfer efficiencies. Best fits of the energy transfer data, using a mathematical model developed to account for the presence of domains, indicates the domain size to be in the range 10-20 nm. © 2003 Elsevier Science B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/92482 |
ISSN | 2023 Impact Factor: 5.4 2023 SCImago Journal Rankings: 0.910 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Troup, GM | en_HK |
dc.contributor.author | Tulenko, TN | en_HK |
dc.contributor.author | Lee, SP | en_HK |
dc.contributor.author | Wrenn, SP | en_HK |
dc.date.accessioned | 2010-09-17T10:47:36Z | - |
dc.date.available | 2010-09-17T10:47:36Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Colloids And Surfaces B: Biointerfaces, 2003, v. 29 n. 2-3, p. 217-231 | en_HK |
dc.identifier.issn | 0927-7765 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/92482 | - |
dc.description.abstract | We present evidence that laterally phase separated cholesterol domains constitute a new, equilibrium phase in biological membranes. The domains are characterized in multi-lamellar vesicles (MLV) made of cholesterol and dimyristoylphosphatidylcholine (DMPC) but are also shown to exist in biologically relevant, egg lecithin systems containing a mixture of phospholipids. This work utilizes the fluorescent membrane probes 1-acyl-2-[12-[(5-dimethylamino-1-naphthalenesufonyl)amino]dodecanoyl]-sn- glycero-3-phosphocholine (DANSYL), and ergosta-5,7,9(11),22-tetraen-3β-ol (ERGO), which have been shown to be minimally invasive mimics of native membrane lipids. The highlight of the work is a heating-induced alleviation of a DANSYL blue shift at relatively high (but undersaturated) cholesterol loadings, which is reversible through at least three heating and cooling cycles. Comparison of the DANSYL spectral shifts with published DMPC-cholesterol phase diagrams shows unequivocally that the spectral results cannot be explained in terms of previously understood phase behavior. Rather, a lateral phase separation occurs within the vesicle bilayer, giving rise to cholesterol micro-domains. The cholesterol domains appear to coexist with, and should not be confused with, the well-known liquid-order phase that arises because of the cholesterol condensation effect. Additional studies involving ERGO-DANSYL energy transfer show a sequestration of probes within the bilayer, confirming the DANSYL spectral data, and a model that includes domains provides the best description of measured energy transfer efficiencies. Best fits of the energy transfer data, using a mathematical model developed to account for the presence of domains, indicates the domain size to be in the range 10-20 nm. © 2003 Elsevier Science B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/colsurfb | en_HK |
dc.relation.ispartof | Colloids and Surfaces B: Biointerfaces | en_HK |
dc.subject | Bilayer | en_HK |
dc.subject | Cholesterol | en_HK |
dc.subject | DMPC | en_HK |
dc.subject | Domains | en_HK |
dc.subject | Liquid-ordered | en_HK |
dc.title | Detection and characterization of laterally phase separated cholesterol domains in model lipid membranes | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lee, SP: sumlee@hku.hk | en_HK |
dc.identifier.authority | Lee, SP=rp01351 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S0927-7765(03)00020-1 | en_HK |
dc.identifier.scopus | eid_2-s2.0-0038406406 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0038406406&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 29 | en_HK |
dc.identifier.issue | 2-3 | en_HK |
dc.identifier.spage | 217 | en_HK |
dc.identifier.epage | 231 | en_HK |
dc.identifier.isi | WOS:000183004800016 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Troup, GM=7005997903 | en_HK |
dc.identifier.scopusauthorid | Tulenko, TN=7005077545 | en_HK |
dc.identifier.scopusauthorid | Lee, SP=7601417497 | en_HK |
dc.identifier.scopusauthorid | Wrenn, SP=6603940041 | en_HK |
dc.identifier.issnl | 0927-7765 | - |