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Article: Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis

TitleAnaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis
Authors
KeywordsAnaerobic bacteria
Intrahepatic stone
PCR
Single-strand confromational polymorphism
Issue Date2000
PublisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2000, v. 113 n. 9, p. 858-861 How to Cite?
AbstractObjective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59). The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.
Persistent Identifierhttp://hdl.handle.net/10722/92479
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorLam, Ken_HK
dc.contributor.authorTang, YJen_HK
dc.contributor.authorGumerlock, PHen_HK
dc.contributor.authorLee, DKen_HK
dc.contributor.authorKim, MHen_HK
dc.contributor.authorLee, SPen_HK
dc.contributor.authorSilva Jr, Jen_HK
dc.contributor.authorLeung, JWen_HK
dc.date.accessioned2010-09-17T10:47:30Z-
dc.date.available2010-09-17T10:47:30Z-
dc.date.issued2000en_HK
dc.identifier.citationChinese Medical Journal, 2000, v. 113 n. 9, p. 858-861en_HK
dc.identifier.issn0366-6999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92479-
dc.description.abstractObjective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59). The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.en_HK
dc.languageengen_HK
dc.publisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/en_HK
dc.relation.ispartofChinese Medical Journalen_HK
dc.subjectAnaerobic bacteriaen_HK
dc.subjectIntrahepatic stoneen_HK
dc.subjectPCRen_HK
dc.subjectSingle-strand confromational polymorphismen_HK
dc.titleAnaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilisen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11776087-
dc.identifier.scopuseid_2-s2.0-0034285814en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034285814&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume113en_HK
dc.identifier.issue9en_HK
dc.identifier.spage858en_HK
dc.identifier.epage861en_HK
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridLiu, Y=7410229885en_HK
dc.identifier.scopusauthoridLam, K=55041298800en_HK
dc.identifier.scopusauthoridTang, YJ=36793999000en_HK
dc.identifier.scopusauthoridGumerlock, PH=7006294328en_HK
dc.identifier.scopusauthoridLee, DK=26029189500en_HK
dc.identifier.scopusauthoridKim, MH=26643177500en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK
dc.identifier.scopusauthoridSilva Jr, J=7403023859en_HK
dc.identifier.scopusauthoridLeung, JW=7202180292en_HK

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