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Article: The Effect of N-Methyl-N′-nitro-N-nitrosoguanidine (MNNG) on Cultured Dog Pancreatic Duct Epithelial Cells

TitleThe Effect of N-Methyl-N′-nitro-N-nitrosoguanidine (MNNG) on Cultured Dog Pancreatic Duct Epithelial Cells
Authors
KeywordsCarcinogen
Cell culture
Mucin secretion
Pancreatic epithelial cells
Issue Date1996
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pancreasjournal.com
Citation
Pancreas, 1996, v. 12 n. 2, p. 109-116 How to Cite?
AbstractTo study the morphologic and genetic events associated with the carcinogenic process in the pancreas, we have isolated and cultured a cell line of dog pancreatic duct epithelial cells and treated these cells with a carcinogen. The pancreatic duct epithelial cells were plated onto Vitrogen-coated Transwell inserts suspended above a feeder layer of human gallbladder myofibroblasts. The epithelial cells grew steadily into polarized monolayers, could be passaged repeatedly, and demonstrated the typical morphologic, immunohistochemical, and flow cytometric profile of normal well-differentiated columnar pancreatic epithelial cells. After being treated with 10-5 M N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) for 48 h, the treated cells grew on plastic surfaces. When grown in organotypic culture, the MNNG-treated cells were cuboidal with a multilayered, pseudostratified architecture. Flow cytometry demonstrated aneuploidy and a high percentage of the cells in S phase after reaching confluency, in sharp contrast to untreated cells. Cytogenetic analysis of the MNNG-treated cells revealed frequent chromosomal trisomy and tetrasomy. The secretion of mucin was also different in the MNNG-treated cells versus the untreated cells. The cultured pancreatic epithelial cells may be useful as an assay system to study the genotoxicity of known and potential carcinogens.
Persistent Identifierhttp://hdl.handle.net/10722/92478
ISSN
2015 Impact Factor: 2.738
2015 SCImago Journal Rankings: 1.114
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorOda, Den_HK
dc.contributor.authorSavard, CEen_HK
dc.contributor.authorEng, Len_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:47:29Z-
dc.date.available2010-09-17T10:47:29Z-
dc.date.issued1996en_HK
dc.identifier.citationPancreas, 1996, v. 12 n. 2, p. 109-116en_HK
dc.identifier.issn0885-3177en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92478-
dc.description.abstractTo study the morphologic and genetic events associated with the carcinogenic process in the pancreas, we have isolated and cultured a cell line of dog pancreatic duct epithelial cells and treated these cells with a carcinogen. The pancreatic duct epithelial cells were plated onto Vitrogen-coated Transwell inserts suspended above a feeder layer of human gallbladder myofibroblasts. The epithelial cells grew steadily into polarized monolayers, could be passaged repeatedly, and demonstrated the typical morphologic, immunohistochemical, and flow cytometric profile of normal well-differentiated columnar pancreatic epithelial cells. After being treated with 10-5 M N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) for 48 h, the treated cells grew on plastic surfaces. When grown in organotypic culture, the MNNG-treated cells were cuboidal with a multilayered, pseudostratified architecture. Flow cytometry demonstrated aneuploidy and a high percentage of the cells in S phase after reaching confluency, in sharp contrast to untreated cells. Cytogenetic analysis of the MNNG-treated cells revealed frequent chromosomal trisomy and tetrasomy. The secretion of mucin was also different in the MNNG-treated cells versus the untreated cells. The cultured pancreatic epithelial cells may be useful as an assay system to study the genotoxicity of known and potential carcinogens.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pancreasjournal.comen_HK
dc.relation.ispartofPancreasen_HK
dc.subjectCarcinogenen_HK
dc.subjectCell cultureen_HK
dc.subjectMucin secretionen_HK
dc.subjectPancreatic epithelial cellsen_HK
dc.titleThe Effect of N-Methyl-N′-nitro-N-nitrosoguanidine (MNNG) on Cultured Dog Pancreatic Duct Epithelial Cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8720655-
dc.identifier.scopuseid_2-s2.0-0030070392en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030070392&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue2en_HK
dc.identifier.spage109en_HK
dc.identifier.epage116en_HK
dc.identifier.isiWOS:A1996TQ85000001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridOda, D=7006186359en_HK
dc.identifier.scopusauthoridSavard, CE=6701738621en_HK
dc.identifier.scopusauthoridEng, L=25942882000en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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