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Article: Expression of cytokine and chemokine mRNA and secretion of tumor necrosis factor-α by gallbladder epithelial cells: Response to bacterial lipopolysaccharides

TitleExpression of cytokine and chemokine mRNA and secretion of tumor necrosis factor-α by gallbladder epithelial cells: Response to bacterial lipopolysaccharides
Authors
KeywordsMolecular Sequence Numbers
Issue Date2002
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcgastroenterol/
Citation
Bmc Gastroenterology, 2002, v. 2 How to Cite?
AbstractBackground: In addition to immune cells, many other cell types are known to produce cytokines. Cultured normal mouse gallbladder epithelial cells, used as a model system for gallbladder epithelium, were examined for their ability to express the mRNA of various cytokines and chemokines in response to bacterial lipopolysaccharide. The synthesis and secretion of the tumor necrosis factor-α (TNF-α) protein by these cells was also measured. Results: Untreated mouse gallbladder cells expressed mRNA for TNF-α,RANTES, and macrophage inflammatory protein-2 (MIP-2). Upon treatment with lipopolysaccharide, these cells now produced mRNA for Interleukin-1β (IL-1β), IL-6, monocyte chemoattractant protein-1 (MCP-1), and showed increased expression of TNF-α and MIP-2 mRNA. Untreated mouse gallbladder cells did not synthesize TNF-α protein; however, they did synthesize and secrete TNF-α upon treatment with lipopolysaccharide. Methods: Cells were treated with lipopolysaccharides from 3 strains of bacteria. Qualitative and semi-quantitative RT-PCR, using cytokine or chemokine-specific primers, was used to measure mRNA levels of TNFα, IL-1β, IL-6, IL-10, KC, RANTES, MCP-1, and MIP-2. TNF-α protein was measured by immunoassays. Conclusion: This research demonstrates that gallbladder epithelial cells in response to lipopolysaccharide exposure can alter their cytokine and chemokine RNA expression pattern and can synthesize and secrete TNFα protein. This suggests a mechanism whereby gallbladder epithelial cells in vivo may mediate gallbladder secretory function, inflammation and diseases in an autocrine/paracrine fashion by producing and secreting cytokines and/or chemokines during sepsis. © 2002 Savard et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/92476
ISSN
2015 Impact Factor: 2.101
2015 SCImago Journal Rankings: 0.999
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorSavard, CEen_HK
dc.contributor.authorBlinman, TAen_HK
dc.contributor.authorChoi, HSen_HK
dc.contributor.authorLee, SKen_HK
dc.contributor.authorPandol, SJen_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:47:24Z-
dc.date.available2010-09-17T10:47:24Z-
dc.date.issued2002en_HK
dc.identifier.citationBmc Gastroenterology, 2002, v. 2en_HK
dc.identifier.issn1471-230Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92476-
dc.description.abstractBackground: In addition to immune cells, many other cell types are known to produce cytokines. Cultured normal mouse gallbladder epithelial cells, used as a model system for gallbladder epithelium, were examined for their ability to express the mRNA of various cytokines and chemokines in response to bacterial lipopolysaccharide. The synthesis and secretion of the tumor necrosis factor-α (TNF-α) protein by these cells was also measured. Results: Untreated mouse gallbladder cells expressed mRNA for TNF-α,RANTES, and macrophage inflammatory protein-2 (MIP-2). Upon treatment with lipopolysaccharide, these cells now produced mRNA for Interleukin-1β (IL-1β), IL-6, monocyte chemoattractant protein-1 (MCP-1), and showed increased expression of TNF-α and MIP-2 mRNA. Untreated mouse gallbladder cells did not synthesize TNF-α protein; however, they did synthesize and secrete TNF-α upon treatment with lipopolysaccharide. Methods: Cells were treated with lipopolysaccharides from 3 strains of bacteria. Qualitative and semi-quantitative RT-PCR, using cytokine or chemokine-specific primers, was used to measure mRNA levels of TNFα, IL-1β, IL-6, IL-10, KC, RANTES, MCP-1, and MIP-2. TNF-α protein was measured by immunoassays. Conclusion: This research demonstrates that gallbladder epithelial cells in response to lipopolysaccharide exposure can alter their cytokine and chemokine RNA expression pattern and can synthesize and secrete TNFα protein. This suggests a mechanism whereby gallbladder epithelial cells in vivo may mediate gallbladder secretory function, inflammation and diseases in an autocrine/paracrine fashion by producing and secreting cytokines and/or chemokines during sepsis. © 2002 Savard et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcgastroenterol/en_HK
dc.relation.ispartofBMC Gastroenterologyen_HK
dc.subjectMolecular Sequence Numbersen_HK
dc.titleExpression of cytokine and chemokine mRNA and secretion of tumor necrosis factor-α by gallbladder epithelial cells: Response to bacterial lipopolysaccharidesen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1471-230X-2-23en_HK
dc.identifier.pmid12377103-
dc.identifier.pmcidPMC130965-
dc.identifier.scopuseid_2-s2.0-18744401105en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18744401105&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume2en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSavard, CE=6701738621en_HK
dc.identifier.scopusauthoridBlinman, TA=6602329840en_HK
dc.identifier.scopusauthoridChoi, HS=34876536500en_HK
dc.identifier.scopusauthoridLee, SK=7601392865en_HK
dc.identifier.scopusauthoridPandol, SJ=35465340400en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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