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Article: Estimating the size of laterally phase separated cholesterol domains in model membranes with Förster resonance energy transfer: A simulation study

TitleEstimating the size of laterally phase separated cholesterol domains in model membranes with Förster resonance energy transfer: A simulation study
Authors
KeywordsBilayer
Cholesterol
Domains
FRET
Membranes
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/colsurfb
Citation
Colloids And Surfaces B: Biointerfaces, 2004, v. 33 n. 1, p. 57-65 How to Cite?
AbstractIn this work, we use two vertically-coupled square two-dimensional lattices to simulate membrane bilayers containing a uniform size distribution of cholesterol immiscible domains of a predetermined size distribution. We substitute cholesterols and phospholipids with their fluorescent analogs and calculate the efficiency of energy transfer as a function of acceptor concentration for four membrane configurations. The simulated efficiency of energy transfer as a function of acceptor concentration data is then fit with an analytical FRET model to estimate the domain size, in the same manner in which experimental FRET data is analyzed. The fitted model parameters (domain size and donor partition coefficient) are compared to the simulation inputs to test the applicability of the FRET model to estimating the size of laterally phase separated cholesterol domains. We show that the FRET model yields good size estimates for domains that range between 1 and 25nm. We also find that the assumed fluorophore configuration in the FRET model leads to a constant under-prediction of these values. Finally, we demonstrate that when two parameters are open to the fit, the FRET model adequately predicts the donor partition coefficient in addition to the domain size. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92475
ISSN
2015 Impact Factor: 3.902
2015 SCImago Journal Rankings: 1.105
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTroup, GMen_HK
dc.contributor.authorTulenko, TNen_HK
dc.contributor.authorLee, SPen_HK
dc.contributor.authorWrenn, SPen_HK
dc.date.accessioned2010-09-17T10:47:23Z-
dc.date.available2010-09-17T10:47:23Z-
dc.date.issued2004en_HK
dc.identifier.citationColloids And Surfaces B: Biointerfaces, 2004, v. 33 n. 1, p. 57-65en_HK
dc.identifier.issn0927-7765en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92475-
dc.description.abstractIn this work, we use two vertically-coupled square two-dimensional lattices to simulate membrane bilayers containing a uniform size distribution of cholesterol immiscible domains of a predetermined size distribution. We substitute cholesterols and phospholipids with their fluorescent analogs and calculate the efficiency of energy transfer as a function of acceptor concentration for four membrane configurations. The simulated efficiency of energy transfer as a function of acceptor concentration data is then fit with an analytical FRET model to estimate the domain size, in the same manner in which experimental FRET data is analyzed. The fitted model parameters (domain size and donor partition coefficient) are compared to the simulation inputs to test the applicability of the FRET model to estimating the size of laterally phase separated cholesterol domains. We show that the FRET model yields good size estimates for domains that range between 1 and 25nm. We also find that the assumed fluorophore configuration in the FRET model leads to a constant under-prediction of these values. Finally, we demonstrate that when two parameters are open to the fit, the FRET model adequately predicts the donor partition coefficient in addition to the domain size. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/colsurfben_HK
dc.relation.ispartofColloids and Surfaces B: Biointerfacesen_HK
dc.subjectBilayeren_HK
dc.subjectCholesterolen_HK
dc.subjectDomainsen_HK
dc.subjectFRETen_HK
dc.subjectMembranesen_HK
dc.titleEstimating the size of laterally phase separated cholesterol domains in model membranes with Förster resonance energy transfer: A simulation studyen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.colsurfb.2003.08.016en_HK
dc.identifier.scopuseid_2-s2.0-0347359169en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0347359169&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue1en_HK
dc.identifier.spage57en_HK
dc.identifier.epage65en_HK
dc.identifier.isiWOS:000188512400009-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridTroup, GM=7005997903en_HK
dc.identifier.scopusauthoridTulenko, TN=7005077545en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK
dc.identifier.scopusauthoridWrenn, SP=6603940041en_HK

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