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Article: Mucin secretion by the human colon cell line LS174T is regulated by bile salts

TitleMucin secretion by the human colon cell line LS174T is regulated by bile salts
Authors
KeywordsBile salt
Colon
Mucin
Obstructive jaundice
Protein kinase C
Issue Date1999
PublisherOxford University Press. The Journal's web site is located at http://glycob.oxfordjournals.org/
Citation
Glycobiology, 1999, v. 9 n. 1, p. 13-19 How to Cite?
AbstractWe recently reported that bile salts play a role in the regulation of mucin secretion by cultured dog gallbladder epithelial cells. In this study we have examined whether bile salts also influence mucin secretion by the human epithelial colon cell line LS174T. Solutions of bile salts were applied to monolayers of LS174T cells. Mucin secretion was quantified by measuring the secretion of [3H]GlcNAc labeled glycoproteins. Both unconjugated bile salts as well as taurine conjugated bile salts stimulated mucin secretion by the colon cells in a dose-dependent fashion. Hydrophobic bile salts were more potent stimulators than hydrophilic bile salts. Free (unconjugated) bile salts were more stimulatory compared with their taurine conjugated counterparts. Stimulation of mucin secretion by LS174T cells was found to occur at much lower bile salt concentrations than in the experiments with the dog gallbladder epithelial cells. The protein kinase C activators PMA and PDB had no stimulatory effect on mucin secretion. We conclude that mucin secretion by the human colon epithelial cell line LS174T is regulated by bile salts. We suggest that regulation of mucin secretion by bile salts might be a common mechanism, by which different epithelia protect themselves against the detergent action of bile salts, to which they are exposed throughout the gastrointestinal tract.
Persistent Identifierhttp://hdl.handle.net/10722/92468
ISSN
2015 Impact Factor: 3.283
2015 SCImago Journal Rankings: 1.692
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKlinkspoor, JHen_HK
dc.contributor.authorMok, KSen_HK
dc.contributor.authorVan Klinken, BJWen_HK
dc.contributor.authorTytgat, GNJen_HK
dc.contributor.authorLee, SPen_HK
dc.contributor.authorGroen, AKen_HK
dc.date.accessioned2010-09-17T10:47:09Z-
dc.date.available2010-09-17T10:47:09Z-
dc.date.issued1999en_HK
dc.identifier.citationGlycobiology, 1999, v. 9 n. 1, p. 13-19en_HK
dc.identifier.issn0959-6658en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92468-
dc.description.abstractWe recently reported that bile salts play a role in the regulation of mucin secretion by cultured dog gallbladder epithelial cells. In this study we have examined whether bile salts also influence mucin secretion by the human epithelial colon cell line LS174T. Solutions of bile salts were applied to monolayers of LS174T cells. Mucin secretion was quantified by measuring the secretion of [3H]GlcNAc labeled glycoproteins. Both unconjugated bile salts as well as taurine conjugated bile salts stimulated mucin secretion by the colon cells in a dose-dependent fashion. Hydrophobic bile salts were more potent stimulators than hydrophilic bile salts. Free (unconjugated) bile salts were more stimulatory compared with their taurine conjugated counterparts. Stimulation of mucin secretion by LS174T cells was found to occur at much lower bile salt concentrations than in the experiments with the dog gallbladder epithelial cells. The protein kinase C activators PMA and PDB had no stimulatory effect on mucin secretion. We conclude that mucin secretion by the human colon epithelial cell line LS174T is regulated by bile salts. We suggest that regulation of mucin secretion by bile salts might be a common mechanism, by which different epithelia protect themselves against the detergent action of bile salts, to which they are exposed throughout the gastrointestinal tract.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://glycob.oxfordjournals.org/en_HK
dc.relation.ispartofGlycobiologyen_HK
dc.subjectBile salten_HK
dc.subjectColonen_HK
dc.subjectMucinen_HK
dc.subjectObstructive jaundiceen_HK
dc.subjectProtein kinase Cen_HK
dc.titleMucin secretion by the human colon cell line LS174T is regulated by bile saltsen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/glycob/9.1.13en_HK
dc.identifier.pmid9884402-
dc.identifier.scopuseid_2-s2.0-0032891886en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032891886&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue1en_HK
dc.identifier.spage13en_HK
dc.identifier.epage19en_HK
dc.identifier.isiWOS:000077881400003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKlinkspoor, JH=6602590656en_HK
dc.identifier.scopusauthoridMok, KS=7103141173en_HK
dc.identifier.scopusauthoridVan Klinken, BJW=6602574356en_HK
dc.identifier.scopusauthoridTytgat, GNJ=35966168300en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK
dc.identifier.scopusauthoridGroen, AK=35242574800en_HK

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