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Article: Interactions between sphingomyelin and cholesterol in low density lipoproteins and model membranes

TitleInteractions between sphingomyelin and cholesterol in low density lipoproteins and model membranes
Authors
KeywordsAggregation
Cholesterol
LDL
Rafts
Sphingomyelin
Sphingomyelinase
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jcis
Citation
Journal Of Colloid And Interface Science, 2006, v. 293 n. 1, p. 203-212 How to Cite?
AbstractThis work examines three related, but previously unexplored, aspects of membrane biophysics and colloid science in the context of atherosclerosis. First, we show that sphingomyelinase (SMase)-induced aggregation of low density lipoproteins (LDLs), coupled with LDL exposure to cholesterol esterase (CEase), results in nucleation of cholesterol crystals, long considered the hallmark of atherosclerosis. In particular, this study reveals that the order of enzyme addition does not effect the propensity of LDL to nucleate cholesterol crystals, raising the possibility that nucleation can proceed from either the intra- or extracellular space. Second, we demonstrate that ceramide-rich aggregates of LDL release cholesterol to neighboring vesicles far more rapidly, and to a greater extent, than does native LDL. A likely explanation for this observation is displacement of cholesterol from SM-Chol rafts by "raft-loving" ceramide. Third, we demonstrate that a time-independent Förster resonance energy transfer (FRET) assay, based on dehydroergosterol and dansylated lecithin and used previously to study cholesterol nanodomains, can be used to measure raft sizes (on the order of 10 nm) in model membrane systems. Taken together, these observations point to the possibility of an extracellular nucleation mechanism and underscore the important role that biological colloids play in human disease. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92467
ISSN
2015 Impact Factor: 3.782
2015 SCImago Journal Rankings: 1.126
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuarino, AJen_HK
dc.contributor.authorLee, SPen_HK
dc.contributor.authorWrenn, SPen_HK
dc.date.accessioned2010-09-17T10:47:08Z-
dc.date.available2010-09-17T10:47:08Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Colloid And Interface Science, 2006, v. 293 n. 1, p. 203-212en_HK
dc.identifier.issn0021-9797en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92467-
dc.description.abstractThis work examines three related, but previously unexplored, aspects of membrane biophysics and colloid science in the context of atherosclerosis. First, we show that sphingomyelinase (SMase)-induced aggregation of low density lipoproteins (LDLs), coupled with LDL exposure to cholesterol esterase (CEase), results in nucleation of cholesterol crystals, long considered the hallmark of atherosclerosis. In particular, this study reveals that the order of enzyme addition does not effect the propensity of LDL to nucleate cholesterol crystals, raising the possibility that nucleation can proceed from either the intra- or extracellular space. Second, we demonstrate that ceramide-rich aggregates of LDL release cholesterol to neighboring vesicles far more rapidly, and to a greater extent, than does native LDL. A likely explanation for this observation is displacement of cholesterol from SM-Chol rafts by "raft-loving" ceramide. Third, we demonstrate that a time-independent Förster resonance energy transfer (FRET) assay, based on dehydroergosterol and dansylated lecithin and used previously to study cholesterol nanodomains, can be used to measure raft sizes (on the order of 10 nm) in model membrane systems. Taken together, these observations point to the possibility of an extracellular nucleation mechanism and underscore the important role that biological colloids play in human disease. © 2005 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jcisen_HK
dc.relation.ispartofJournal of Colloid and Interface Scienceen_HK
dc.subjectAggregationen_HK
dc.subjectCholesterolen_HK
dc.subjectLDLen_HK
dc.subjectRaftsen_HK
dc.subjectSphingomyelinen_HK
dc.subjectSphingomyelinaseen_HK
dc.titleInteractions between sphingomyelin and cholesterol in low density lipoproteins and model membranesen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jcis.2005.06.043en_HK
dc.identifier.pmid16019021-
dc.identifier.scopuseid_2-s2.0-27744469697en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27744469697&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume293en_HK
dc.identifier.issue1en_HK
dc.identifier.spage203en_HK
dc.identifier.epage212en_HK
dc.identifier.isiWOS:000233675600023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGuarino, AJ=7103082239en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK
dc.identifier.scopusauthoridWrenn, SP=6603940041en_HK

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