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Article: Effect of vitamins A, C and E on normal and HPV-immortalized human oral epithelial cells in culture

TitleEffect of vitamins A, C and E on normal and HPV-immortalized human oral epithelial cells in culture
Authors
KeywordsAntioxidants
Differentiation
Epithelial proliferation
Oral cancer
Issue Date1999
PublisherInternational Institute of Anticancer Research. The Journal's web site is located at http://ar.iiarjournals.org/
Citation
Anticancer Research, 1999, v. 19 n. 6 B, p. 5469-5474 How to Cite?
AbstractThere is experimental and epidemiological evidence that antioxidant vitamins can inhibit carcinogenesis. Since immortalization by Human Papilloma Virus (HPV) is one possible early step towards carcinogenesis in oral epithelia, we studied the differential effect of vitamins A, C and E on HPV-immortalized oral epithelial cells (IHGK) as compared to the normal counterpart. The dose response was determined by morphology, cell cycle by flow cytometry, and growth curve by cell number. The optimum dose in terms of inhibitory effect vs. toxicity was determined for each vitamin by morphology. Optimum doses were: vitamin A - 1.4 x 10 -5 M, vitamin C - 10 -3 M, and vitamin E - 10 -6 M for both HPV-immortalized and normal cells. Growth curve showed reduction of proliferation by all three vitamins, with vitamins A and E more effective than C for both cell types. Flow cytometry showed that vitamins A and E reduced the percentage of cells at G2 phase of cell cycle and indicated arrest in the S phase. This effect was greatest in the immortalized cells with a 50% and 35% decrease of G2 for vitamins A and E respectively, whereas the normal counterpart showed a 48% decrease for A and a 12% increase for E. By organotypic culture, the morphology was not markedly different between the vitamin-treated and the control cells, except for a slight increase in the keratinization of normal cells with vitamin A. Also noted was a reduction in number of cell layers from five layers or more for controls to only one or two for vitamin E. In conclusion, we have demonstrated that the antioxidant vitamins inhibit proliferation, and show a preferential effect on IHGK cells.
Persistent Identifierhttp://hdl.handle.net/10722/92464
ISSN
2015 Impact Factor: 1.895
2015 SCImago Journal Rankings: 0.815
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMason, Ben_HK
dc.contributor.authorGhanee, Nen_HK
dc.contributor.authorHaigh, WGen_HK
dc.contributor.authorLee, SPen_HK
dc.contributor.authorOda, Den_HK
dc.date.accessioned2010-09-17T10:47:02Z-
dc.date.available2010-09-17T10:47:02Z-
dc.date.issued1999en_HK
dc.identifier.citationAnticancer Research, 1999, v. 19 n. 6 B, p. 5469-5474en_HK
dc.identifier.issn0250-7005en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92464-
dc.description.abstractThere is experimental and epidemiological evidence that antioxidant vitamins can inhibit carcinogenesis. Since immortalization by Human Papilloma Virus (HPV) is one possible early step towards carcinogenesis in oral epithelia, we studied the differential effect of vitamins A, C and E on HPV-immortalized oral epithelial cells (IHGK) as compared to the normal counterpart. The dose response was determined by morphology, cell cycle by flow cytometry, and growth curve by cell number. The optimum dose in terms of inhibitory effect vs. toxicity was determined for each vitamin by morphology. Optimum doses were: vitamin A - 1.4 x 10 -5 M, vitamin C - 10 -3 M, and vitamin E - 10 -6 M for both HPV-immortalized and normal cells. Growth curve showed reduction of proliferation by all three vitamins, with vitamins A and E more effective than C for both cell types. Flow cytometry showed that vitamins A and E reduced the percentage of cells at G2 phase of cell cycle and indicated arrest in the S phase. This effect was greatest in the immortalized cells with a 50% and 35% decrease of G2 for vitamins A and E respectively, whereas the normal counterpart showed a 48% decrease for A and a 12% increase for E. By organotypic culture, the morphology was not markedly different between the vitamin-treated and the control cells, except for a slight increase in the keratinization of normal cells with vitamin A. Also noted was a reduction in number of cell layers from five layers or more for controls to only one or two for vitamin E. In conclusion, we have demonstrated that the antioxidant vitamins inhibit proliferation, and show a preferential effect on IHGK cells.en_HK
dc.languageengen_HK
dc.publisherInternational Institute of Anticancer Research. The Journal's web site is located at http://ar.iiarjournals.org/en_HK
dc.relation.ispartofAnticancer Researchen_HK
dc.subjectAntioxidantsen_HK
dc.subjectDifferentiationen_HK
dc.subjectEpithelial proliferationen_HK
dc.subjectOral canceren_HK
dc.titleEffect of vitamins A, C and E on normal and HPV-immortalized human oral epithelial cells in cultureen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10697579en_HK
dc.identifier.scopuseid_2-s2.0-0033367188en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033367188&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue6 Ben_HK
dc.identifier.spage5469en_HK
dc.identifier.epage5474en_HK
dc.identifier.isiWOS:000085317300087-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridMason, B=7202224065en_HK
dc.identifier.scopusauthoridGhanee, N=6505767196en_HK
dc.identifier.scopusauthoridHaigh, WG=6603814152en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK
dc.identifier.scopusauthoridOda, D=7006186359en_HK

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